BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2855.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 651 0.0 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 145 6e-36 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 108 7e-25 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 651 bits (1680), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/447 (75%), Positives = 383/447 (85%) Query: 1 MSLHHRSQAWMALLLRYRRRFLQAWRDRHLQPEGFFNQQEAEFLPAALSLQEAPDSPSLR 60 MSL HR QAW L RY R F AWR R FFN+ EAEFLPAALSLQEAPDS SLR Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTARLLVAMVLLALAWSLIGQIDIVVSANGKVIPSARTKTIASVEVASVRAILVREGQFV 120 WTARLLV MV+ AL WSL+G+IDIVVSANGKVIPSARTKTIASV+V++VRAILV EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 QAGQVLLELDASSSDAERDKAADAVAQARLQAARAAAMMQAVQQLRAPELQDVADVSLAQ 180 +AG+VL+ELD+SSSDAERDKAADAVAQARLQAARA AMM+A+Q LR PEL V V+ AQ Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 WESVRRQLQGQYQDFRARLDRFDDEIVRYTKALRLASQRATDYQELMADHTVSRHAWLEK 240 WE+VR QL QYQDFRARL R DDEI RY AL+LA+QRA+DYQ L+ADHTVSRHAWLEK Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARVDLQGQLTDARNQRAALLAQTQKEAHDSRLEAEKIIEAAQKDQRRAQERSKLLHLR 300 EQARVDLQGQL DARNQRA L+AQT+K+AHDSR+EA+K IEA+Q+DQRRA E SKLL L Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLNVHTIGGVVPAAQPLMQIVPEDGEIEVEAWLDNKDVGFVEVGQEVEVKVD 360 APV+GTVQQL VHTIGGVVPAAQPLMQIVP+D +IEVEA L+NKDVGFVE+GQ EVK+D Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AFDYTKYGTVAGRVVQVSRDAIQDEKKGLVYSTRILLSRHTLGVDGRLLPLTAGLAVNVG 420 AFDYTKYGT+ RVV +SRDAIQDEKKGL+YS RI+L + +L VDGR + L+AG+A NV Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IRTGSRRVIEYALSPLLRHQKEALHER 447 I+TG+RRVIEYALSPL+RHQKEALHER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 145 bits (366), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 114/397 (28%), Positives = 196/397 (49%), Gaps = 29/397 (7%) Query: 58 SLRWTARLLVAMVLLALAWSLIGQIDIVVSANGKVIPSARTKTIASVEVASVRAILVREG 117 S +W RL +A VL+ L W+ + QID V A G+VI +RT+++ + + V+ ILV+EG Sbjct: 12 SAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEG 71 Query: 118 QFVQAGQVLLELDASSSDAERDKAADAVAQARLQAARAAAMMQAVQQLRAPELQDVADVS 177 + V+ GQ+L+ L+ + + VA R+ AR A + P+L D Sbjct: 72 EAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLD----- 126 Query: 178 LAQWESVRRQLQGQYQDFRARLDRFDDEIVRYTKALRLASQRATDYQELMADHTVSRHAW 237 + + Q + R D ++ L LA+Q + L V R Sbjct: 127 ------YKEYIANQKDLYVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180 Query: 238 LEKEQARVDLQGQLTDARNQRAALLAQTQKEAHDSRLEAEKIIEAAQKDQRRAQERSKLL 297 L ++ D++ Q+ + RN K ++ ++ K+ E + + ++R+++L Sbjct: 181 LRLQRNVADIRAQINNKRN----------KYFQEALVDMTKVQEDLNTQREQLEDRTQVL 230 Query: 298 ---HLRAPVSGTVQQLNVHTIGGVVPAAQPLMQIVPEDGEIEVEAWLDNKDVGFVEVGQE 354 L+AP G V+ + V TIGGVV + +M+I+P D I VEA + D +V+VGQ Sbjct: 231 EHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAYVKVGQA 289 Query: 355 VEVKVDAFDYTKYGTVAGRVVQVSRDAIQDE-KKGLVYSTRILLSRHTLGVDGR---LLP 410 VK+DA+DY+ YGT+ G VV VS D + DE ++G + R+ ++ GR + Sbjct: 290 ANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKADAIQ 349 Query: 411 LTAGLAVNVGIRTGSRRVIEYALSPLLRHQKEALHER 447 + G+ V I+ +R V+ + P+ + +E++ ER Sbjct: 350 VKPGMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 108 bits (271), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 109/402 (27%), Positives = 184/402 (45%), Gaps = 35/402 (8%) Query: 76 WSLIGQIDIVVSANGKVIPSARTKTIASVEVASVRAILVREGQFVQAGQVLLELDASSSD 135 WS+ ID+VV +G+++ S + I +E V AILVREG V+ GQ L+ + ++ Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 136 AERDKAADAVAQARLQAARAAAMMQAVQQLRAPELQDVADVS--------------LAQW 181 A+ + + R + AR +A L+ P+ + D + L Q Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQSEQAAFAARSSKLMQE 177 Query: 182 ESVRRQLQGQYQDFRARLDRFDDEIVRYTKALRLASQRATDYQELMADHTVSRHAWLEKE 241 SV R+ Q Q A L L LA ++ +++ + S+ ++ Sbjct: 178 MSVLREQSAQRQ---AELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAA 234 Query: 242 QARVDLQGQL--TDARNQR-AALLAQTQKEAHDS----RLEAEKIIEAAQKDQRRAQERS 294 D Q +L T+A R A +A+ Q + ++ R +A + AA+ + R QE Sbjct: 235 SRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEI 294 Query: 295 KLLH-------LRAPVSGTVQQLNVHTIGGVVPAAQPLMQIVPEDGEIEVEAWLDNKDVG 347 K + ++AP+ G V ++ ++TIGGVV P++++ P D ++ +EA + D Sbjct: 295 KSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRA 354 Query: 348 FVEVGQEVEVKVDAFDYTKYGTVAGRVVQVSRDAIQDE---KKGLVYSTRILLSRHTLGV 404 + G VKV A+DY YG + G V ++S D + DE + Y +I + R + Sbjct: 355 ELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDEL 414 Query: 405 DGRLLPLTAGLAVNVGIRTGSRRVIEYALSPLLRHQKEALHE 446 +L L G+ + I G R V +Y +SPL R + AL E Sbjct: 415 QQKLA-LMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,199,319 Number of extensions: 43912 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 5 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)