BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2851.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 626 e-180 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 140 2e-34 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 110 2e-25 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 626 bits (1615), Expect = e-180, Method: Compositional matrix adjust. Identities = 315/447 (70%), Positives = 383/447 (85%) Query: 1 MSLRHRLQASHALWRRYYSVFRHFWTIRRSLKNDFFNQDEAQFLPAALALQEAPDSKSLR 60 MSLRHR QA LWRRY VF H W R ++DFFN+DEA+FLPAAL+LQEAPDS+SLR Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTGRALMLIVVFALLWSIFGRMDIIVNATGKVIPSARTKTIASVDVSSVKALHVREGQAV 120 WT R L+ +V+FALLWS+ GR+DI+V+A GKVIPSARTKTIASVDVS+V+A+ V EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 RAGDVLIELDSSSSDAEHDKASDTVAQARLQAARASALILAVQNRRAPNLPAIDGVERYQ 180 +AG+VLIELDSSSSDAE DKA+D VAQARLQAARA A++ A+QN R P L + GV + Q Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 WEAVSHQLDGQYQDFMARLRRMDEEIARYGAALPLASQRALDYQALVADQTVSRHAWLER 240 WEAV QL QYQDF ARL R+D+EI+RYGAAL LA+QRA DYQ LVAD TVSRHAWLE+ Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARIDLKGQLAEAISQRASLIAQTLKEAHDARIEATRVIESAQQDQRRAREHSRLLKLI 300 EQAR+DL+GQL +A +QRA+L+AQT K+AHD+RIEA + IE++QQDQRRA EHS+LLKL+ Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLNVHTVGGVVAAAQPLMQIVPEDTEVEVEAFLENRDVGFLQVGQEARVKID 360 APV+GTVQQL VHT+GGVV AAQPLMQIVP+D+++EVEA LEN+DVGF+++GQ A VKID Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AFDYTKYGTLAARVRHVSRDAIPEEKRGLLYSTKIVLDKNTLVVDGKSLPVLAGMSVSVE 420 AFDYTKYGTL ARV H+SRDAI +EK+GLLYS +IVLD+++L+VDG+ + + AGM+ +VE Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IKTGTRRVIEYVLSPLMRHQREALNER 447 IKTG RRVIEY LSPL+RHQ+EAL+ER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 140 bits (354), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 117/399 (29%), Positives = 206/399 (51%), Gaps = 33/399 (8%) Query: 58 SLRWTGRALMLIVVFALLWSIFGRMDIIVNATGKVIPSARTKTIASVDVSSVKALHVREG 117 S +W R M V+ LLW+ ++D + A G+VI +RT+++ + D VK + V+EG Sbjct: 12 SAQWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEG 71 Query: 118 QAVRAGDVLIELDSSSSDAEHDKASDTVAQARLQAARASALILAVQNRRAPNLPAIDGVE 177 +AV+ G +L+ L+ + + VA R+ AR A + P+L +D Sbjct: 72 EAVKQGQLLMVLEQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDL--LD--- 126 Query: 178 RYQWEAVSHQLDGQYQDFMARLRRMDEEIARYGAALPLASQRALDYQALVADQTVSRHAW 237 Y+ E +++Q D ++ R +D+++A L LA+Q ++L V R Sbjct: 127 -YK-EYIANQKDL----YVKRKILIDQDLASLQDMLGLANQELEMNKSLERTGDVGRADV 180 Query: 238 LEREQARIDLKGQLAEAISQRASLIAQTLKEAHDARIEATRVIESAQQDQRRAREHSRLL 297 L ++ D++ Q+ + K +A ++ T+V E + + + +++L Sbjct: 181 LRLQRNVADIRAQINNKRN----------KYFQEALVDMTKVQEDLNTQREQLEDRTQVL 230 Query: 298 ---KLIAPVSGTVQQLNVHTVGGVVAAAQPLMQIVPEDTEVEVEAFLENRDVGFLQVGQE 354 +L AP G V+ + V T+GGVV + +M+I+P D + VEA + D +++VGQ Sbjct: 231 EHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAYVKVGQA 289 Query: 355 ARVKIDAFDYTKYGTLAARVRHVSRDAIPEEKR-GLL--YSTKIVLDKNTLVVDGK---S 408 A VK+DA+DY+ YGTL V +VS D + +E R G + Y I L++ V G+ + Sbjct: 290 ANVKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEE--AVFKGRKADA 347 Query: 409 LPVLAGMSVSVEIKTGTRRVIEYVLSPLMRHQREALNER 447 + V GM+ +VEIK TR V+ ++ P+ + +E++ ER Sbjct: 348 IQVKPGMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 109/400 (27%), Positives = 189/400 (47%), Gaps = 31/400 (7%) Query: 76 WSIFGRMDIIVNATGKVIPSARTKTIASVDVSSVKALHVREGQAVRAGDVLIELDSSSSD 135 WS+F +D++V +G+++ S + I ++ V A+ VREG AV+ G LI + ++ Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 136 AEHDKASDTVAQARLQAARASALILAVQNRRAPNL-----PAIDGVERYQWEAVSHQLDG 190 A+ + + R + AR SA + + P+ PA+ E+ + A S +L Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDPAVQS-EQAAFAARSSKLMQ 176 Query: 191 QY----QDFMARLRRMDEEIARY---GAALPLAS-QRALDYQALVAD--------QTVSR 234 + + R + E I+R + L LA Q L + LV + Q SR Sbjct: 177 EMSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAASQLEDIQAASR 236 Query: 235 ----HAWLEREQARID-LKGQLAEAISQRASLIAQTLKEAHDARIEATRVIESAQQDQRR 289 + L A I L +AEA +++ ++ +A A + Q++ + Sbjct: 237 VQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAETELARLQEEIKS 296 Query: 290 AREHSRLLKLIAPVSGTVQQLNVHTVGGVVAAAQPLMQIVPEDTEVEVEAFLENRDVGFL 349 + ++ AP+ G V ++ ++T+GGVV P++++ P D ++ +EA + D L Sbjct: 297 RNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARISPTDRAEL 356 Query: 350 QVGQEARVKIDAFDYTKYGTLAARVRHVSRDAIPEE--KRG-LLYSTKIVLDKNTLVVDG 406 G ARVK+ A+DY YG + V +S D +P+E RG Y KI + + + Sbjct: 357 VTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVKRGHDELQQ 416 Query: 407 KSLPVLAGMSVSVEIKTGTRRVIEYVLSPLMRHQREALNE 446 K L ++ GM+ S +I G R V +Y++SPL R + AL E Sbjct: 417 K-LALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.131 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,268,758 Number of extensions: 48601 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 5 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)