BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2835.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 759 0.0 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 275 6e-75 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 241 1e-64 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 157 1e-39 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 142 3e-35 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 759 bits (1961), Expect = 0.0, Method: Compositional matrix adjust. Identities = 368/382 (96%), Positives = 375/382 (98%) Query: 3 MLLSPEHEMIRDAMRQFAQERLLPFAADWDKNHTFPAEALKELAALGAMGMCVPEQWGGA 62 MLLSPEHEMIRDAMR FAQERLLPFAADWD+NHTFPAEALKELA LGAMGMCVPEQWGGA Sbjct: 1 MLLSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGA 60 Query: 63 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPAQKEQWLKPLARGEMLGC 122 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSP QKEQWLKPLARGEMLGC Sbjct: 61 GMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGC 120 Query: 123 FCLTEPHTGSDAAAIRTRAERDGEYFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS 182 FCLTEPHTGSDAAAIRTRAERDG+ FILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS Sbjct: 121 FCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGIS 180 Query: 183 CFLVPRDTAGFVVGRSEEKMGQHASDTVQIILENCRVPATALLGKEGDGYRIALSNLEAG 242 CFLVP DT GFVVGRSEEKMGQHASDTVQI+L+NCRVPATALLGKEG+GYRIALSNLEAG Sbjct: 181 CFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAG 240 Query: 243 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIDHQAVNFRLADMNTLLDAARLMVWRA 302 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPII+HQAVNFRLADMNTLLDAARLMVWRA Sbjct: 241 RIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRA 300 Query: 303 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 362 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY Sbjct: 301 AQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIY 360 Query: 363 EGANDIQRLVIGRAIASDQVHS 384 EGANDIQRLVIGRAIASDQ H+ Sbjct: 361 EGANDIQRLVIGRAIASDQAHA 382 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 275 bits (702), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 150/378 (39%), Positives = 226/378 (59%), Gaps = 3/378 (0%) Query: 1 MDMLLSPEHEMIRDAMRQFAQERLLPFAADWDKNHTFPAEALKELAALGAMGMCVPEQWG 60 MD L+ + + + R FA + PFAA WD+ FP + + + LG G+ PE G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 61 GAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPAQKEQWLKPLARGEML 120 G G+ + + EE+A +T+ +++ N ++ I +G P+ K QW + LA G + Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIA-SWGQPSLKAQWCEQLAAGRKI 119 Query: 121 GCFCLTEPHTGSDAAAIRTRAERDGEYFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKG 180 G +CLTEP +GSDAA+++T A + +++LNG+K FI+ V +V A T G +G Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-GEGARG 178 Query: 181 ISCFLVPRDTAGFVVGRSEEKMGQHASDTVQIILENCRVPATALLGKEGDGYRIALSNLE 240 +S +VP + G G+ E KMG ++ T I +N +VPA LLG+EG G+ A+ L+ Sbjct: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 Query: 241 AGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIDHQAVNFRLADMNTLLDAARLMVW 300 GRI IA SVG A+AA +AA +Y ++R+ FG PI D QA+ F+LADM T L AAR MV Sbjct: 239 GGRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVR 298 Query: 301 RAAQLKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRIC 359 AA D G P +MAK A++ ++ ++A+Q+HGG GY ++P+ER +RDVR+ Sbjct: 299 LAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVH 358 Query: 360 QIYEGANDIQRLVIGRAI 377 QI EG N+I R++I R + Sbjct: 359 QILEGTNEIMRVIISRKL 376 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 122/377 (32%), Positives = 223/377 (59%), Gaps = 6/377 (1%) Query: 12 IRDAMRQFAQERLLPFAADWDKNHTFPAEALKELAALGAMGMCVPEQWGGAGMDYMSLVL 71 +R+A+ FA + P AA+ D++ FP + K+L LG +G+ V E++GGAG+ Y++ ++ Sbjct: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 Query: 72 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPAQKEQWLKPLARGEMLGCFCLTEPHTG 131 A+EEI+ + ++L ++ G+ QK ++L L G+ +G ++EP+ G Sbjct: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAG 137 Query: 132 SDAAAIRTRAERDGEYFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPRDTA 191 SD +++ RA++ G+ ++LNG+K +IT+G A V +V+A TD AG +G++ FLV + Sbjct: 138 SDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGMTAFLVEKGYK 197 Query: 192 GFVVGRSEEKMGQHASDTVQIILENCRVPATALLGKEGDGYRIALSNLEAGRIGIAAQSV 251 GF V + +K+G S T +++ ++C VP +LG G G + +S L+ R ++ + Sbjct: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPL 257 Query: 252 GMARAAFEAAVSYARQRESFGVPIIDHQAVNFRLADMNTLLDAARLMVWRAAQLKDQG-- 309 G+ +A + V Y R+ FG I + Q + +LADM + + A + V+ Q D+ Sbjct: 258 GIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADS 317 Query: 310 ----RPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGA 365 R K+A+ A ++++E A +A +AIQ GG GY +++PV R++RD ++ +I G Sbjct: 318 ADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLWRDAKLYEIGAGT 377 Query: 366 NDIQRLVIGRAIASDQV 382 ++I+R++IGR + +D + Sbjct: 378 SEIRRMLIGRELFADTL 394 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 157 bits (398), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 121/373 (32%), Positives = 194/373 (52%), Gaps = 31/373 (8%) Query: 6 SPEHEMIRDAMRQFAQERLLPFAADWDKNHTFPAEALKELAALGAMGMCVPEQWGGAGMD 65 S H +RDA+R + + D+ +P + + L G + +P+++GG+G+ Sbjct: 7 SDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLG 66 Query: 66 YMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPAQKEQWLKPLARGE 118 + +EEI G NS AC G ++GSP QKE++L +A GE Sbjct: 67 LTEASVIMEEINRTGG---------NSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGE 117 Query: 119 M-LGCFCLTEPHTGSDAAAIRTRAERDGEYFILNGTKQFITSGKHAGVAIVFAVTDR--- 174 + L +TEP TG+D I+T A + G+ +++NG K +I+ +H+ + I+ A T Sbjct: 118 LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQ 177 Query: 175 -SAGKKGISCFLVPRDTAGFVVGRSEE-----KMGQHASDTVQIILENCRVPATALLGKE 228 S +G+S FLV A +G E M H +T ++ +N +PA L+G+E Sbjct: 178 VSRKSEGMSIFLVDLKEA---IGHGMEVRPILNMVNH--ETNELFFDNLEIPAENLIGEE 232 Query: 229 GDGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIDHQAVNFRLADM 288 G G+R L L A R IAA+ +G + A YAR+R F PI +Q V F LA+ Sbjct: 233 GRGFRYILDGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEA 292 Query: 289 NTLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFP 348 ++AA LM ++A L D + C +A+MAK A++AA + A+ +Q HGG G+ ++ Sbjct: 293 YIEVEAANLMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYD 352 Query: 349 VERIYRDVRICQI 361 VER +R+ R+ Q+ Sbjct: 353 VERKFRETRLYQV 365 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 142 bits (359), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 119/350 (34%), Positives = 167/350 (47%), Gaps = 42/350 (12%) Query: 54 CVPEQWGGAGMDYMSLVLALEEI--AAGDGATSTIVSVQNSLACGITEKYGSPAQKEQWL 111 C PE GG G+ ++ L AL E+ AA G T + + AC +GS A + ++L Sbjct: 97 CAPEH-GGQGLPHV-LDCALYEMLSAANHGWTMYPGLLHGAYAC--LAAHGSAALQARYL 152 Query: 112 KPLARGEMLGCFCLTEPHTGSDAAAIRTRA--ERDGEYFILNGTKQFITSGKH----AGV 165 +A GE L CLTE GSD +RTRA + DG Y L+G K FI+ G+H V Sbjct: 153 DKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYR-LSGDKIFISGGEHDLTPNIV 211 Query: 166 AIVFA-VTDRSAGKKGISCFLVPR-----DTAGFVVGRSEEKMGQHASDTVQIILENCRV 219 +V A + D AG KG+S FL PR G E KMG H S T + E + Sbjct: 212 HLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQ- 270 Query: 220 PATALLGKEGDGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVP----- 274 L+G+ G G + A R+ + AQ +G+A AA++ A +YAR+R P Sbjct: 271 --AWLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGN 328 Query: 275 --IIDHQAVNFRLADMNTLLDAARLMVWRAAQLKDQGRPCLKEA-------------SMA 319 I+ H AV L + ++ ARL+ A L D + A + Sbjct: 329 DLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVI 388 Query: 320 KMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGANDIQ 369 K F ++ + AS A+Q+ GG GY + +E+ RD RI IYEG N+IQ Sbjct: 389 KAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQ 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,140,213 Number of extensions: 46454 Number of successful extensions: 195 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 13 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)