BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2823.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312118|ref|YP_003776210.1| hypothetical protein Hsero_2809... 209 1e-55 >gi|300312118|ref|YP_003776210.1| hypothetical protein Hsero_2809 [Herbaspirillum seropedicae SmR1] Length = 132 Score = 209 bits (533), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 96/132 (72%), Positives = 115/132 (87%) Query: 1 MDIATPALQSLRIEMHFDEMPLSSGTGFIARRRNGMYLITNRHNVTGRDQHTGACLSPQS 60 M++A P+LQS RIEM+F ++ LSSGT FI RR + ++L +N HNVTGRDQH GACLS + Sbjct: 1 MNVAPPSLQSSRIEMYFGDILLSSGTSFITRRGSKLFLTSNSHNVTGRDQHAGACLSARE 60 Query: 61 GIPNNVVIRYYKADSPGEFVSYREPLLANDEPLWFEHPELGTTADFVALKVTNAPGAIIH 120 GIPNNVVIRY KAD+PGEF+SY+EP+LANDEPLWF+HP+LG TADFVALK+TN+PGAIIH Sbjct: 61 GIPNNVVIRYNKADNPGEFLSYQEPILANDEPLWFKHPKLGKTADFVALKLTNSPGAIIH 120 Query: 121 PIDPASVGVPIK 132 PIDP SVGVP K Sbjct: 121 PIDPVSVGVPTK 132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 933,271 Number of extensions: 44750 Number of successful extensions: 118 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 1 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)