BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2803.p (396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine... 706 0.0 gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine... 168 7e-43 gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine... 68 1e-12 >gi|300312108|ref|YP_003776200.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 706 bits (1821), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/399 (88%), Positives = 370/399 (92%), Gaps = 3/399 (0%) Query: 1 MSHATGPLAGITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYDARVDGLSS 60 MS + PL GITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYD+RV+GLSS Sbjct: 1 MSPSHRPLEGITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYDSRVNGLSS 60 Query: 61 HFVWTNRSKESLTLNLKDEAGKRIIDQLLQQADVLVQNLAPGAAAALGLSAQVLHERHPR 120 HFVWTNRSKESLTLNLKDE G+RIIDQLLQQADVLVQNLAPGAAA LGLSAQ LH RHPR Sbjct: 61 HFVWTNRSKESLTLNLKDEEGRRIIDQLLQQADVLVQNLAPGAAAGLGLSAQALHARHPR 120 Query: 121 LIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTRDDVAKAGCSIADIAAGMYAYSN 180 LIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGT DDV KAGCSIADIAAGMYAYSN Sbjct: 121 LIVCDISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTPDDVVKAGCSIADIAAGMYAYSN 180 Query: 181 ILAALIQRGRDGRG---SHIDLSMLEALAEWMSFPLYYAYQGASAPQRSGAEHATIYPYG 237 ILAALI+RGR+GR SHIDLSMLE+LAEWMSFPLYYAYQGASAP RSGAEHATIYPYG Sbjct: 181 ILAALIKRGREGREGRGSHIDLSMLESLAEWMSFPLYYAYQGASAPLRSGAEHATIYPYG 240 Query: 238 PFTAGDGKTVMLGLQNEREWKVFCEVVLEDAALASDARFSSNALRHEHRETLRQLILTMF 297 PF+AGDGKTVMLGLQNEREWKVFCE VL+DAALASDARFSSNA RHEHR+ LRQLIL +F Sbjct: 241 PFSAGDGKTVMLGLQNEREWKVFCEQVLQDAALASDARFSSNARRHEHRDVLRQLILDVF 300 Query: 298 ADMTAEQVVARLDQAQIANARVNTMHDLWQHPQLAARQRWTEVDTPVGKVAAMLPPGAND 357 A ++AEQV ARLD+AQIANARVNTM +LWQHPQLAARQRWTEV TPVGKVAA+LPPGAND Sbjct: 301 AGLSAEQVAARLDEAQIANARVNTMQELWQHPQLAARQRWTEVATPVGKVAALLPPGAND 360 Query: 358 RYDYRMEAIPALGQHTEAILGQLGYDAAGITALRERGAV 396 YDYRMEAIPALGQHTE+IL LGY+A I ALRERGAV Sbjct: 361 SYDYRMEAIPALGQHTESILSALGYEAGQIAALRERGAV 399 >gi|300312971|ref|YP_003777063.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 168 bits (426), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 128/412 (31%), Positives = 199/412 (48%), Gaps = 26/412 (6%) Query: 6 GPLAGITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYDARVDGLSSHFVWT 65 GPLAGI V+ L IA PFC+R LA+ GA VIK+E PG GD R + DG S + Sbjct: 9 GPLAGIKVLELGTLIAGPFCSRMLAEFGADVIKVEAPGNGDPLRQWRVLKDGTSLWWSVQ 68 Query: 66 NRSKESLTLNLKDEAGKRIIDQLLQQADVLVQNLAPGAAAALGLSAQVLHERHPRLIVCD 125 R+K+S+TLN+KDE + I +L +ADV+++N PG L + L + +P I+ Sbjct: 69 ARNKKSITLNMKDERAQEIARKLALEADVIIENYRPGVLEKWKLGYEDLKQLNPATIMVR 128 Query: 126 ISGYGEDGPYRDKKAYDLLIQSEAGFLSVTGTRD-DVAKAGCSIADIAAGMYAYSNILAA 184 +SGYG+ GP RD + + +S G V+G D + G SI D A ++ + A Sbjct: 129 LSGYGQTGPLRDLPGFGAIGESMGGIRYVSGHPDRPPVRIGISIGDSIAALHGVIGAMMA 188 Query: 185 LIQR-----------GRD---GRGSHIDLSMLEALAEWM-SFPLYYAYQGASAPQRSGAE 229 L R G D G+G +D+++ EA+ M S + + G +R+G Sbjct: 189 LRHRDVTGGRWNGKQGADCVAGQGQMVDVALYEAVFNMMESMVPEFDFAGV-VRERTGGA 247 Query: 230 HATIYPYGPFTAGDGKTVMLGLQNEREWK-VFCEVVLEDAALASDARFSSNALRHEHRET 288 I P +T G+G +++ + +K + + ED +A+D + N R E Sbjct: 248 LPGIVPSNTYTTGEGMNIVIAGNGDAIFKRLMSAIGRED--MANDPGLARNDGRVPRTEE 305 Query: 289 LRQLILTMFADMTAEQVVARLDQAQIANARVNTMHDLWQHPQLAARQRWTEVD----TPV 344 + I + T E +A L A + ++ ++ D+ Q AR + + TPV Sbjct: 306 IDAAIQQWCSTQTIETALATLQAADVPVGKIYSVKDMMNDAQFLARDMFEQHQFADGTPV 365 Query: 345 GKVAAMLPPGANDRYDYRMEAIPALGQHTEAILGQLGYDAAGITALRERGAV 396 K+ + P + + P LG H++ IL LGYD A I LR++G V Sbjct: 366 -KLPGITPKMSETPGQTKWLG-PELGAHSDEILQSLGYDEAHIKMLRDQGVV 415 >gi|300310600|ref|YP_003774692.1| acyl-CoA transferases/carnitine dehydratase [Herbaspirillum seropedicae SmR1] Length = 469 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 49/145 (33%), Positives = 65/145 (44%), Gaps = 12/145 (8%) Query: 7 PLAGITVISLEHAIAAPFCTRQLADLGARVIKIERPGVGDFARGYDARVDGLSSHFVWTN 66 PL GI V+ L +A P TR LA LGA V++I+ Y V Sbjct: 207 PLRGIRVLDLTRILAGPVATRFLAGLGAEVLRID---------PYGWEEPNTVPEVVLGK 257 Query: 67 RSKESLTLNLKDEAGKRIIDQLLQQADVLVQNLAPGAAAALGLSAQVLHERHPRLIVCDI 126 R LNLKD A + +L+ AD+LV A A LGL A E +P LI + Sbjct: 258 RCAR---LNLKDPADLDTLRRLMGHADILVHGYRSDALAKLGLDAAQRRELNPALIDVSL 314 Query: 127 SGYGEDGPYRDKKAYDLLIQSEAGF 151 YG G ++ ++ +D LIQ G Sbjct: 315 DAYGWSGAWQCRRGFDSLIQMSTGI 339 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,232,955 Number of extensions: 50756 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 3 Length of query: 396 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 309 Effective length of database: 1,207,925 Effective search space: 373248825 Effective search space used: 373248825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)