BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2801.p (374 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 701 0.0 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 219 4e-58 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 133 2e-32 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 87 3e-18 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 86 4e-18 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 701 bits (1810), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/374 (93%), Positives = 363/374 (97%) Query: 1 MKIHAAVLREMTEAHPFAVSQPLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRP 60 MKI AAVLREM++AHPFAVSQPLKIE+LDLDPPGPGEVLV+MKAAGLCHSDLSVITGVRP Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 61 RPVPMALGHEASAEVVQVGSGVTDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 RPVPMALGHEASAEVV+VGSGV DLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN Sbjct: 61 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 Query: 121 TVGQLLSGQRRLHDGSGHVHHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAVLT 180 TVG+LLSGQRRL D + VHHHLGVSAFADHAVVS RSCVKIEADIDPV+AALFGCAVLT Sbjct: 121 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT 180 Query: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGAT 240 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKL +AR+LGAT Sbjct: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGAT 240 Query: 241 ATVNARDPDVVEKVKALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPNQNW 300 ATVNARDPD VEKV+ALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAP Q+W Sbjct: 241 ATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHW 300 Query: 301 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINRGFD 360 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGL+QAGKLPVD+LMG+RLALADINRGFD Sbjct: 301 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFD 360 Query: 361 RLHSGEGLRDLIVF 374 RLH GEGLRDLIVF Sbjct: 361 RLHGGEGLRDLIVF 374 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 219 bits (557), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 134/377 (35%), Positives = 200/377 (53%), Gaps = 12/377 (3%) Query: 1 MKIHAAVLREMTEAHPFAVSQPLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRP 60 MK AAV + +PL IE+++L P GEVLV++KA G+CH+D ++G P Sbjct: 1 MKTRAAV--------AWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP 52 Query: 61 RPV-PMALGHEASAEVVQVGSGVTDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 + P LGHE + VV VG GV L+ D V+ ++ P C C C+ + LC+ AT Sbjct: 53 EGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRAT 112 Query: 120 NTVGQLLSGQRRLHDGSGHVHHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAVL 179 G + R + H++G S F+++ VV + KI +D + GC V Sbjct: 113 QGRGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVT 172 Query: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGA 239 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ ++ +AR G Sbjct: 173 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGM 232 Query: 240 TATVNARD-PDVVEKVKALTRGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPN 297 T +N +D P+VV+ + +T GG DY+FE G+ M + +G G ++ G+ A Sbjct: 233 TDFINPKDVPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAG 292 Query: 298 QNWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINR 357 Q + + LV R KGS G D+P+ + Y GKL +D L+ +L L IN Sbjct: 293 QEISTRPFQLVT-GRVWKGSAFGGARGRTDVPKIVDWYMEGKLNIDDLITHKLPLERINE 351 Query: 358 GFDRLHSGEGLRDLIVF 374 GFD + SGE +R ++ F Sbjct: 352 GFDLMKSGESIRSVVEF 368 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 114/368 (30%), Positives = 179/368 (48%), Gaps = 45/368 (12%) Query: 3 IHAAVLREMTEAHPFAVSQPLKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVITG---VR 59 + AAV+RE +PL IEQ+ + P PG++LVK +A+G+CH+DL G V+ Sbjct: 5 MKAAVVREF--------GKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVK 56 Query: 60 PRPVPMALGHEASAEVVQVGSGVTDLRAGDLVVLVFV-PSCGHCLPCMEGRPALCEPGAA 118 P P P GHE + V VG+GV ++ GD V + ++ +CG C PC G LC A Sbjct: 57 PTP-PFIPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLC---AE 112 Query: 119 TNTVGQLLSGQRRLHDGSGHVHHHLGVSAFADHAVVSSRSCVKIEADIDPVEAALFGCAV 178 G ++G +FA++ + + + ++D AA CA Sbjct: 113 QQNTGYSVNG------------------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAG 154 Query: 179 LTGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALG 238 +T V + EV+ G + G+GG+G A+ A A G V+A D+H+ KL +A+ LG Sbjct: 155 VT-VYKGLKETEVRPGEWVVISGIGGLGHMAVQYAKAMGMH-VVAADIHEDKLALAKKLG 212 Query: 239 ATATVNARDPDVVEKVKALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPNQ 298 A TV+ R+ + V +V+ + GG A A S +AME A+ R G GLP Sbjct: 213 ADLTVDGRNHNAVAEVQRII-GGAHGALVTAVSPKAMEQAFGFLRARGTMALVGLPP--G 269 Query: 299 NWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINRG 358 + +L + V + TV+GS +G+ +D+ + GK+ + L +IN Sbjct: 270 DISLPVFNTVLKRITVRGSIVGT---RQDLEESLVFAAEGKVAAHFTWDK---LDNINAI 323 Query: 359 FDRLHSGE 366 F R+ G+ Sbjct: 324 FARMEEGK 331 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 102/357 (28%), Positives = 147/357 (41%), Gaps = 48/357 (13%) Query: 37 EVLVKMKAAGLCHSDLSVITGVRP-RPVPMALGHEASAEVVQVGSGVTDLRAGDLVVLVF 95 +V++K+ A +C SDL + G P LGHE VV+ G + L+ GD VV+ F Sbjct: 27 DVILKVTATAICGSDLHLYRGKIPATKAGDILGHEFMGVVVEAGPAIKRLKKGDRVVVPF 86 Query: 96 VPSCGHCLPCMEGRPALCEPGAATNTVGQLLSGQRRLHDGS---GHVHHHLGVSAFADHA 152 V +CG C C + A CE TN L + + G+ G+ H + G+ Sbjct: 87 VIACGQCFFCDQHLYAACE---TTNPDRGALMNAKGIRSGAAMFGYSHLYGGMPG----- 138 Query: 153 VVSSRSCVKI-EADIDPV--------EAALFGCAVLTGVGAAVNTAEVKAGTTAAVLGLG 203 V++ +AD+ P+ E LF +L AV A V+ G++ A+ G G Sbjct: 139 --GQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQAVKNAGVREGSSVAIYGAG 196 Query: 204 GVGLCAMLGALASGAREVIAVDLHDSKLEVARALGATATVNARDPDVVEKVKALTRG-GV 262 VG A A GA + VD H +L A+ VN D E + A T G GV Sbjct: 197 PVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVNFDQVDPAEYIIANTAGRGV 256 Query: 263 D-----YAFEMAGSV---------------QAMEAAYRMTRRGGMTVTAGLPAPNQNWAL 302 D FE GSV QA+ RRGG+ G+ A + L Sbjct: 257 DGVIDAVGFEAKGSVLETAMTTLKLEGSSGQALRHCIASVRRGGVISVPGVYAGFIHGFL 316 Query: 303 QQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLPVDKLMGQRLALADINRGF 359 + + TVK G +P + + G+L D ++ RL L D RG+ Sbjct: 317 FG-DIFEKGVTVK---TGQTHVQHYMPELLASIEEGRLRPDAIITHRLPLEDAARGY 369 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 81/276 (29%), Positives = 120/276 (43%), Gaps = 24/276 (8%) Query: 23 LKIEQLDLDPPGPGEVLVKMKAAGLCHSDLSVI----TGVRPRPVPMALGHEASAEVVQV 78 L+I+ LD P ++ VK+KA G+C SDL GV PM LGHE S ++ +V Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 79 GSGVTDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNTVGQLLSGQRRLHDGSGH 138 G+ VTD+ G + + CG C C EG+ C R + + Sbjct: 73 GTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCL--------------DMRFYGSAMR 118 Query: 139 VHHHLGVSAFADHAVVSSRSCVKIEAD-IDPVEAALFGCAVLTGVGAAVNTAEVKAGTTA 197 H G AF ++ RS + AD + EAA+ L+ AV A G Sbjct: 119 TPHVQG--AFRQEIIIE-RSQAHVVADHVSDGEAAM--AEPLSVALHAVRRAGPLVGKRV 173 Query: 198 AVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLEVARALGATATVNARDPDVVEKVKAL 257 V G G +G ++ A +GA EV+ D+ L+ A +GA + +N + K A Sbjct: 174 LVTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAA 233 Query: 258 TRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGL 293 +G D FE +G+ +A+ A R + V GL Sbjct: 234 DKGTFDVLFEASGNERALVGALEALRPQAIIVQVGL 269 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,068,669 Number of extensions: 42950 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 7 Length of query: 374 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 287 Effective length of database: 1,207,925 Effective search space: 346674475 Effective search space used: 346674475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)