BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2763.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 681 0.0 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 133 2e-32 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 129 3e-31 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 114 9e-27 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 94 2e-20 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 681 bits (1758), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/349 (94%), Positives = 338/349 (96%) Query: 1 MQYRQLGRTDAKVSLITLGTMTWGEQNTEAQAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 MQYR LGRTD KVSLITLGTMTWGEQN+EA AHAQLDYAVERGINLIDVAEMYPVPPKAE Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHIRGGINDLDRKGLTEALHGS 120 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRH+RGGIN+LDRKGLTEALHGS Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVADETTVPIEETLKVLSEFVKAGKIRYIG 180 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHV DE+TVPIEETL VLSEFVKAGKIR IG Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEVNLAEFAHREQVGLLAYSPLA 240 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFE+NLAEFAHREQVGLLAYSPLA Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 241 FGMLSGKYLDGARPAGGRLTLFERFSRYTNPQAEGATAQYVALARQHGLDPAQMALAYVN 300 FGMLSGKYLDGARPAGGRLTLFERFSRYTN QAE ATA+YVALARQHGLDPAQMALAYVN Sbjct: 241 FGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVN 300 Query: 301 SRPFLTSNIIGATTLEQLRSNIDSAQLQLSEEVLAGIEAIHRKQPNPAP 349 SRPFLTSNIIGATTL QLRSNIDSA+LQL E+VLA IEAIHR+QPNPAP Sbjct: 301 SRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHRRQPNPAP 349 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 133 bits (334), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 119/353 (33%), Positives = 171/353 (48%), Gaps = 64/353 (18%) Query: 4 RQLGRTDAKVSLITLGTMTWGE---QNTEAQAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 R+LG VS I LG M + Q +A++ A +D A+E GINL+D A+MY Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMY------- 70 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHIRGGINDLDRKG--LTEALH 118 E LG +K G+RD+ IATK G R P +P G +D + +A+ Sbjct: 71 GPYTNEELLGRAIK--GRRDKFFIATKF-GIRRDPSDP-----GARGVDGSPAYIRQAVE 122 Query: 119 GSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVADETTVPIEETLKVLSEFVKAGKIRY 178 GSL+RL ++ +DLY YQH D T PIE T+ VL++ V+AGKIRY Sbjct: 123 GSLKRLGVETIDLY----------------YQHRIDPAT-PIEVTMGVLADLVQAGKIRY 165 Query: 179 IGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEVNLAEFAHREQVGLLAYSP 238 IGLS +A A RA H + + +Q+ Y+L R E + +G +AYSP Sbjct: 166 IGLSEASA---ATLERA---HRVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSP 219 Query: 239 LAFGMLSG---KYLDGARPAGGRLTLFERFSRYTNPQAEGAT-------AQYVA-LARQH 287 L G L+G ++ D L E R +NP+ +G A+ V +A QH Sbjct: 220 LGRGFLTGAIQRFED----------LAEDDFRRSNPRFQGENFARNLQLAEKVKEMAGQH 269 Query: 288 GLDPAQMALAYVNSRPFLTSNIIGATTLEQLRSNIDSAQLQLSEEVLAGIEAI 340 G P+Q+ALA+V ++ I G L N S ++L+ E L ++AI Sbjct: 270 GCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQALDAI 322 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 98/348 (28%), Positives = 171/348 (49%), Gaps = 46/348 (13%) Query: 1 MQYRQLGRTDAKVSLITLGTMTWGEQNTEAQAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 M YR+LG+++ KVS + LG+M +G+Q ++ + A E G+N ID A++Y Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVY------- 53 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKC-TGPARKPHNPRHIRGGINDLDRKGLTEALHG 119 T+G +E +G L +G+R + ++A+K + KP+ ++ R + Sbjct: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNE--------SNYSRSWIVRETEN 103 Query: 120 SLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVADETTVPIEETLKVLSEFVKAGKIRYI 179 L RL D++D+ +H + DE +EE ++ L + ++ GKIR Sbjct: 104 ILTRLDTDYLDILYMH--------------RDYQDEN---LEEAVQALGDLIRVGKIRGF 146 Query: 180 GLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEVNLAEFAHREQVGLLAYSPL 239 G+SN W +A+ +R E++ + + Q YNLLNR EV + +G++ YSP+ Sbjct: 147 GVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPI 206 Query: 240 AFGMLSGKYLDGARPA-------GGRLTLFERFSRYTNPQAEGATAQYVALARQHGLDPA 292 A G+L+GKY G +PA G + + F + A+ A G+ Sbjct: 207 ARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFREESLLIAQRLQQHCEA----RGVKLG 262 Query: 293 QMALAYVNSRPFLTSNIIGATTLEQLRSNIDSAQLQLSEEVLAGIEAI 340 Q A A+V + ++S I G TL Q+ D+A+L++S E A ++++ Sbjct: 263 QFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISAEEEALVDSL 310 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 114 bits (286), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 108/353 (30%), Positives = 164/353 (46%), Gaps = 55/353 (15%) Query: 1 MQYRQLGRTDAKVSLITLGTM-TWGEQNTEAQAHAQLDYAVERGINLIDVAEMYPVPPKA 59 M YR+ GR+ + I+LG +G + A L A +RG+ D+A Y P Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPP--- 70 Query: 60 ETQGLTERYLGTWLKKS--GKRDQLLIATKC-----TGPARKPHNPRHIRGGINDLDRKG 112 G E G W+ K RD+L+I++K GP P R K Sbjct: 71 --AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGAR-----------KH 117 Query: 113 LTEALHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVADETTVPIEETLKVLSEFVK 172 L + SL+R+ LD+VD++ Y H D T P+EET+ L+ + Sbjct: 118 LIASCEQSLKRMGLDYVDIF----------------YSHRVDADT-PLEETMGALAHLHR 160 Query: 173 AGKIRYIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEVNLAEFAHREQVG 232 GK Y+G+S+ + + + G+ ++ N Y++LNR+ E L VG Sbjct: 161 QGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPN-YSMLNRAIEGGLLPALEELGVG 219 Query: 233 LLAYSPLAFGMLSGKYLDGARPAGGRLTLFERFSRYTNPQ---AEGATAQYVAL---ARQ 286 + +SPLA G+L+ KYL G P G R+T PQ +E A+ AL A + Sbjct: 220 CIGFSPLAQGLLTSKYLGGV-PEGARVTRAPSL-----PQRMLSEDNLARIRALNEIASK 273 Query: 287 HGLDPAQMALAYVNSRPFLTSNIIGATTLEQLRSNIDSA-QLQLSEEVLAGIE 338 G AQMA+A+V +TS +IGA T+EQL ++D+ +L ++E LA I+ Sbjct: 274 RGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAVNKLDFTQEELASID 326 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 107/362 (29%), Positives = 167/362 (46%), Gaps = 54/362 (14%) Query: 1 MQYRQLGRTDAKVSLITLGTM----TWGEQNTEAQAHAQLDYAVERGINLIDVAEMYPVP 56 M+ R+ GRT A+VS I G +WG+ + + A A L A++ G++ ID A++Y Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 Query: 57 PKAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHIRGGINDLDRKGLTEA 116 G +E+ + LK+ G ++ +ATK A + +P H+ G ND R L+ Sbjct: 57 ----GDGRSEQLIAQVLKERGG-ERPFVATK----AGRRLSP-HVASGYND--RAQLSAF 104 Query: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVADETTVPIEETLKVLSEFVKAGKI 176 + SL+ L +D +DL QLH P V YQ +E L V AGKI Sbjct: 105 IDRSLKNLGVDCLDLVQLHCPPTEVY------YQ----------QEVFGHLDALVAAGKI 148 Query: 177 RYIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEVNLAEFAHREQVGLLAY 236 R+ G+S E V + L+A E ++ + I N + R + L E A R + ++A Sbjct: 149 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPISLFLQE-AQRRDIAVIAR 202 Query: 237 SPLAFGMLSGKYLDGARPA----------GGRLTLFERFSRYTNPQAEGATAQYVALARQ 286 PLA G+L+GK A G + E FS A A + AL Q Sbjct: 203 VPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ 262 Query: 287 HGLDPAQMALAYVNSRPFLTSNIIGATTLEQLRSNIDSAQL-QLSEEVLAGIEAIHRKQP 345 G+ AQ AL ++ +++ I GA + Q +SN ++ L ++ EV+ + AI+ Sbjct: 263 -GVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALPPITAEVMQALRAIYLHDI 321 Query: 346 NP 347 P Sbjct: 322 AP 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,099,862 Number of extensions: 45457 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 6 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)