BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2755.p (710 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 532 e-152 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 236 7e-63 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 224 3e-59 gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspiri... 197 3e-51 gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 186 6e-48 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 532 bits (1371), Expect = e-152, Method: Compositional matrix adjust. Identities = 267/499 (53%), Positives = 343/499 (68%), Gaps = 11/499 (2%) Query: 9 EKLLSDYQHARILRLTFPHNDAPPSALLVNKLDASEGLSRDFEFTVDLLADNATLELKDL 68 +KL QH RIL+L F ++DAP + LL+N+L+ASE LSRD+ +T++LL+D+A + L + Sbjct: 9 DKLTRQRQHNRILQLFFINDDAPDAELLINRLEASEALSRDYVYTLELLSDDAHIPLDAM 68 Query: 69 QGKLLCVELVRQDGSLRYFTGYVFSFRFVRTDGDIAFYEARLGPWLKYLSLRTNCMLFHG 128 GKLL V L R DG+ R+FTGYV F F+R+DG IAFYEARL PWL +L+ R N +FH Sbjct: 69 MGKLLRVALTRADGTQRHFTGYVERFAFLRSDGGIAFYEARLVPWLAFLASRHNNRIFHH 128 Query: 129 VSLYDQTRSIFQHYSALPEWEFR-RHGEDLPV-TEACQFNESDHNYLSRRWEAAGWHYAY 186 +SL + +IF+ Y W+ + RHG+ PV TE QF+ESD N L RRWE AGWHY Y Sbjct: 129 LSLEGLSAAIFRAYPMHARWDCQLRHGD--PVRTEMFQFDESDSNLLHRRWEDAGWHYHY 186 Query: 187 AHMATGHKLILSDDSTLADPIDGV-EIAFQRHAGSQEEDGIHEWSGERHIVPASVTLTSF 245 H A GH+L LSDDST A PIDG ++ FQRH G+ EEDG+ +WS R P+S L+S+ Sbjct: 187 EHQADGHQLRLSDDSTYAKPIDGTGQVPFQRHGGAIEEDGMAQWSAVRQFQPSSTRLSSY 246 Query: 246 DFKSPWPKTVDVPTRNNQGLILRKEAYEYTGAYGFKDRQDGDHLATQRMEEFEHAGKFFQ 305 DFK+ P+ V+VPT N QG +L+ E YEY GAYGF +R DGD + RMEEFE A + F+ Sbjct: 247 DFKAARPQEVEVPTLNKQGAVLQVEHYEYAGAYGFANRDDGDRQSRLRMEEFEAAAQVFE 306 Query: 306 AAGNNRNVVPGRWFRLTGHFDVGS-----QQEREFLIIEAHHSASNNYHVNNGTAPHYEN 360 GN R + PGR FRLTGHF + ++ E LII HSA+NNY ++ T P Y N Sbjct: 307 GTGNCRFLQPGRTFRLTGHFSGSAASHDHDRDPELLIISVEHSATNNYLQDSETPPFYAN 366 Query: 361 QIKCIRKVIPWRPGRGFNSVEPKIYGLQTATVVGPKGEEIHTDEYGRVKVQFHWDREGEL 420 +++C+RK IPWRPGR + S + G+QTATVVGP GE +H DEYGRVKVQFHWD+ G Sbjct: 367 RVRCVRKRIPWRPGRSYYSQPTALLGIQTATVVGPAGENLHVDEYGRVKVQFHWDQIGRN 426 Query: 421 DERSSAWLRVATAWAGSNFGATFIPRIGCEVIVQFLDGNPDRGLITGMVPNAANMPAWEL 480 DERSSAWLRVA++W+G G +PRIG VIVQ+L G+PDR +ITG V N NMP W L Sbjct: 427 DERSSAWLRVASSWSGGEQGLVSVPRIGQLVIVQWLGGHPDRPIITGSVVNQLNMPPWTL 486 Query: 481 PANKTQSGILTRS-TPKGG 498 P+ SG+ +R P+ G Sbjct: 487 PSQSALSGMRSRELAPRTG 505 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 236 bits (601), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 169/548 (30%), Positives = 274/548 (50%), Gaps = 55/548 (10%) Query: 16 QHARILRLTFPHNDAPPSALLVNKLDASEGLSRDFEFTVDLLADNATLELKDLQGKLLCV 75 Q RIL+L + P LL ++ SE L R +++T+DL++ +ELK L + + + Sbjct: 30 QEKRILKL---DSVLGPDVLLPQRMVGSERLGRGYDYTIDLVSTRDDIELKKLIAQPVTL 86 Query: 76 ELVRQDGS-LRYFTGYVFSFRFVRTDGDIAFYEARLGPWLKYLSLRTNCMLFHGVSLYDQ 134 L +QDGS GYV + R + +DG +A ++ + PWL +L R + ++ D Sbjct: 87 WL-QQDGSDYAPLHGYVHTARRLGSDGQLASFQIGMAPWLHFLKYRKDARIWQDSKADDI 145 Query: 135 TRSIF-QHYSALPEWEFRRHGEDLPVTEACQFNESDHNYLSRRWEAAGWHYAYAHM---A 190 +F QH A + F LP + Q+ E+D +++ R E GW YAY Sbjct: 146 LADVFDQHAYARGNYRFEIVTPALPRSYCTQY-ETDWHFVQRLMEEEGW-YAYHEQKPDG 203 Query: 191 TGHKLILSDDSTLADPIDGVEIAFQRHAGSQEE-DGIHEWSGERHIVPASVTLTSFDFKS 249 +GH L+++D++ A P+ ++AF AG+Q+E + I WS R + + + D+KS Sbjct: 204 SGHVLVITDNTDQAKPLAPEQLAFH-GAGTQDEVNRILHWSASRGLASTRLATRTDDYKS 262 Query: 250 P--WPKTVDVPTRNNQGLILRKEAYEYTGAYGFKDRQDGDHLATQRMEEFEHAGKFFQAA 307 P +T + + L E YEY+G Y + + DGD A R+E++E + K F Sbjct: 263 PGDQKQTQERVLPEHGALPGELEVYEYSGPYSYAKQDDGDRQARLRVEQWESSMKRFMGV 322 Query: 308 GNNRNVVPGRWFRLTGH--FDVGSQQEREFLII--------------------------- 338 R + GRWF LT H S+Q+ +F++I Sbjct: 323 SGARTLAAGRWFTLTDHPAHRGDSKQDSQFMLIAVEWFIENNLPLSGTARDFPGSLKPQL 382 Query: 339 ---EAHHSASNNYHVNNGTAPHYENQIKCIRKVIPWRPGRGFNSVEPKIYGLQTATVVGP 395 +A SN+ N H N+ + R+ +P+R F+ +P ++ QTA VVGP Sbjct: 383 DAFKASMGRSNDDAGGNARTGHCFNRFEVQRRKLPYR--SPFDHTKPVMHP-QTAVVVGP 439 Query: 396 KGEEIHTDEYGRVKVQFHWDREGELDERSSAWLRVATAWAGSNFGATFIPRIGCEVIVQF 455 +GEE+ TD+ RVKVQF WDR DE++S W+RV+ AG N+G +PR+G E+I+ F Sbjct: 440 QGEEVFTDKLNRVKVQFRWDRINPGDEKASCWVRVSYPNAGENWGEVKVPRVGQELIISF 499 Query: 456 LDGNPDRGLITGMVPNAANMPAWELPANKTQSGILTRSTPKGGYHNANALRFEDMKGQEQ 515 L+G+PDR + TG + N P W + SG+ ++ G+ N L +D Q + Sbjct: 500 LNGDPDRPVATGRLFNQEQPPQWH--TDGRLSGLKSKEFGGNGF---NQLVMDDTTSQNR 554 Query: 516 LWLHAERN 523 + L++ ++ Sbjct: 555 VHLYSSQS 562 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 224 bits (570), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 200/749 (26%), Positives = 334/749 (44%), Gaps = 75/749 (10%) Query: 16 QHARILRLTFPHNDAPPSALLVNKLDASEGLSRDFEFTVDLLADNATLELKDLQGKLLCV 75 QH R+L+L + P LL +L A+E L+ +E T+DLLA + + L+ L + + + Sbjct: 18 QHRRLLQLD---SSLGPDILLPQRLVATERLNDGYEVTIDLLATHTGIALEQLIAQPVTL 74 Query: 76 ELVRQDGSLRYFTGYVFSFRFVRTDGDIAFYEARLGPWLKYLSLRTNCMLFHGVSLYDQT 135 + + DG GYV + + + +DG++ F + L PWL +L R + ++ D Sbjct: 75 WIRQHDGDTLPLHGYVHTAKRLGSDGELDFCQLSLSPWLHFLRFRKDARIWQDKRTEDIL 134 Query: 136 RSIF-QHYSALPEWEFRRHGEDLPVTEACQFNESDHNYLSRRWEAAGW--HYAYAHMATG 192 +F H A + F+ P Q+ E+D +++ R E GW H+ +G Sbjct: 135 ADVFNNHPQARGHYRFQLERPYTPRPYCTQY-ETDWHFVQRLMEEEGWFAHHEQREDGSG 193 Query: 193 HKLILSDDSTLADPIDGVEIAFQRHAGSQEEDGIHEWSGERHIVPASVTLTSFDFKSP-W 251 H L+++D++ + IAF A E D I WS R + S D+KSP Sbjct: 194 HVLVITDNTWNLPGLPQQGIAFHGAAPRDELDKILHWSASRTLSSKGWRGRSDDYKSPGM 253 Query: 252 PKTVDVPTRNNQG-LILRKEAYEYTGAYGFKDRQDGDHLATQRMEEFEHAGKFFQAAGNN 310 K + G L + E YEYTGAY F+ ++ G+ A ++E++E + + + A Sbjct: 254 KKEAEEQVMPEYGQLPAQLEMYEYTGAYSFRLQEQGNRQADLQVEQWESSMQRYSAVSGV 313 Query: 311 RNVVPGRWFRLTGHFDVGSQ--QEREFLIIEAHHSASNNY-------------------- 348 RN+ GRWFRL H ++ +ER+F+I+ NN Sbjct: 314 RNLACGRWFRLEEHPQHRAEPDRERQFVILAIDWFVENNLPLSHQALDFPGSLKGALMAF 373 Query: 349 -----------HVNNGTAPHYENQIKCIRKVIPWRPGRGFNSVEPKIYGLQTATVVGPKG 397 +N H N+ + R+ +P+RP R + QTA VVGP Sbjct: 374 KESIRRERQVAETDNEHTGHCFNRFEVQRRKLPFRPARAHAR---PVMLPQTAIVVGPAS 430 Query: 398 EEIHTDEYGRVKVQFHWDREGELDERSSAWLRVATAWAGSNFGATFIPRIGCEVIVQFLD 457 EE++TD R+KVQF WDR +E +S W+RV+ AG +GA +PRIG EVIV FL+ Sbjct: 431 EEVYTDHLDRIKVQFRWDRINPGNEAASCWVRVSYPNAGQYWGAIQVPRIGQEVIVSFLN 490 Query: 458 GNPDRGLITGMVPNAANMPAWELPANKTQSGILTRSTPKGGYHNANALRFEDMKGQEQLW 517 G+PDR ++TG + NA P W N SG+ ++ G+ N + +D Q ++ Sbjct: 491 GDPDRPVVTGRLFNAEQRPQWH--TNGRLSGVKSKEFGGTGF---NQMVMDDTPEQNRIH 545 Query: 518 L-----HAERNQLTEVEHDEDKWVGHDRRK------SIDHDETSHIGHDRTETVDNNETI 566 L HA+ N V G++RR ++ D I + + Sbjct: 546 LYSTNTHAQLNLGHLVSQ-----TGNERRSFFGSGFALSTDAYGAIVTHKGLYISTYGRP 600 Query: 567 TVHNNRTETVDNNETISIGSNRTKTIGKNRKDQIGKNWSTHVARMKTETIGMAYMQNVGM 626 + + + + G+N ++T+ + + + A A ++ Sbjct: 601 GAQGTQLDAREATGQLKSGANLSRTLSETAAKAGAEPLAGQEALRDFIDATQADYEDPSQ 660 Query: 627 GRMENVGLAYNLNV---GTVMATMVGLNQMTKVGKTISITAGEELSISVGKASLRMTADG 683 + A L G + T G++ T G I++++G + SI+VGK+ L A+ Sbjct: 661 AQANRFQQAILLAASPDGIGLTTPKGVH--THAGGEITLSSGADTSIAVGKSLLASVAE- 717 Query: 684 KIFINGSEIGIE---ASGPMQLSGKDVDI 709 KI + + GI+ A G +++ + D+ Sbjct: 718 KISLFAFKAGIKLFSAKGKVEVQAQSDDL 746 >gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 966 Score = 197 bits (501), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 164/572 (28%), Positives = 250/572 (43%), Gaps = 72/572 (12%) Query: 16 QHARILRLTF-PHNDAPPSALLVNKLDASEGLSRDFEFTVDLLADNATLELKDLQGKLLC 74 Q+ R +RL + P +LL+ ++D E L + + ++ + L L L G+ L Sbjct: 19 QNLRPIRLRWGPQEQRFEQSLLLQRVDIQETLLEGMQVQLTCVSTDPDLPLHTLLGQPLG 78 Query: 75 VELVRQDGSLRYFTGYVFSFRFVRTDGDIAFYEARLGPWLKYLSLRTNCMLFHGVSLYDQ 134 VE+V G L + G + DG + Y+ + L + R N +F +S+ Sbjct: 79 VEMVTDRGRLHHTNGLITDACAGHADGALRVYQLTMRDALSIMEGRVNTRIFRRLSVPSI 138 Query: 135 TRSIF----QHYSALPE-WEFRRHGED---LPVTEAC-QFNESDHNYLSR--RWEAAGWH 183 + Q S L ++F G D P E QFNESD +++ R R E W+ Sbjct: 139 LEILLSEWRQRSSTLAAAFKFDLDGLDDTRYPAREISHQFNESDAHFIRRLCRREGIAWY 198 Query: 184 YAYAHMATG-----------HKLILSDDSTLADPIDGVEIAFQRHAGSQEEDGIHEWSGE 232 A A H L+ D L + + R ++E D I WS + Sbjct: 199 VASGQQADETAIATHADIPIHTLVFCDQPALLPRAAAGTVRYHRDGATEERDAISAWSRQ 258 Query: 233 RHIVPASVTLTSFDFKSPWPKTVDVPTRNNQGL-------ILRKEAYEYTGAYGFKDRQD 285 R +VP SV S+D+K+ + + +QG ILR E + + D Sbjct: 259 RSLVPGSVWRRSWDYKAVRAEEASSHSIVDQGDAGYKLANILRDGLIE--APHAADSQND 316 Query: 286 GDHLATQRMEEFEHAGKFFQAAGNNRNVVPGRWFRLTGHFDVGSQQ--EREFLIIEAHHS 343 LA RM E + F A+G R++ G WF L GH D+ Q+ +REFL+ Sbjct: 317 HLRLADTRMLAHEARSESFDASGTVRDLAVGHWFLLDGHPDMRGQKPAQREFLVTSLQQQ 376 Query: 344 ASNNYH-------------------VNNGTAPHYENQIKCIRKVIPWRPGRGFNSVEPKI 384 A NN+ ++ Y NQ+ C R+ P P P + Sbjct: 377 AENNFPTVLQDAIRRLAPASPWKNLAHSAADLRYRNQLTCRRRTAPLAPHYDPRLHLPPV 436 Query: 385 YGLQTATVVGPKGEEIHTDEYGRVKVQF-------HWDREG---ELDERSSAWLRVATAW 434 + + TA VVGP EE++ DE GR+KVQ H +G E+ SA++RV++AW Sbjct: 437 HPI-TALVVGPSKEEVYCDELGRIKVQLQGLHPEDHAHAQGAGASGHEQDSAFVRVSSAW 495 Query: 435 AGSNFGATFIPRIGCEVIVQFLDGNPDRGLITGMVPNAANMPA-----WELPANKTQSGI 489 AG+ +G +PR+G EV++ FL G+PD+ + G++ N N+PA LP N+ SGI Sbjct: 496 AGAGYGHDAVPRVGMEVLIDFLGGDPDKMFVAGVLHNGINLPARFSHSGSLPGNRFLSGI 555 Query: 490 LTRSTPKGGYHNANALRFEDMKGQEQLWLHAE 521 T+ Y N LR +D GQ L +E Sbjct: 556 KTKEIGGARY---NQLRLDDSPGQISTQLASE 584 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 186 bits (472), Expect = 6e-48, Method: Compositional matrix adjust. Identities = 145/539 (26%), Positives = 237/539 (43%), Gaps = 77/539 (14%) Query: 16 QHARILRLTFPHNDAPPSALLVNKLDASEGLSRDFEFTVDLLADNATLELKDLQGKLLCV 75 Q+AR++ +T + P L V + + E ++ +F F +D L+ + L+LK G + + Sbjct: 23 QNARLITMTTAQSAGLPETLAVERFEGEESVNDNFRFDIDALSISTDLDLKQFIGAEISL 82 Query: 76 ELVRQDGSLRYFTGYVFSFRFVRTDGDIAFYEARLGPWLKYLSLRTNCMLFHGVSLYDQT 135 L++ DG R + GY ++ DG +A Y RL +L +L R + LF +++ D Sbjct: 83 RLLQADGGTRAWHGYCTQASWLGADGGLARYRLRLESFLAFLDRRHDSFLFQDMTVTDIA 142 Query: 136 RSIFQHYSALPEWEFRRH-GEDLPVTEAC-QFNESDHNYLSRRWEAAGWHYAYAHMA--- 190 ++ + Y P+ F + L + C Q+ E+D+ +L R + G ++ + H Sbjct: 143 AAVLKEY---PQANFSLEVTQTLAKRDICTQYRETDYAFLRRILASEGLNFRFEHEQDDA 199 Query: 191 ----------TGHKLILSDDSTLADPIDGVE--IAFQRHAGSQEEDGIHEWSGERHIVPA 238 HKL++ D + A + G + I F R A + D I +++ R + P Sbjct: 200 TQQHDQSQPHARHKLVIFDRNAKAPALPGGDEYIRFHRVAAMEASDAITDFAAVRSVRPN 259 Query: 239 SVTLTSFDFKSPWPKTVDVPTRN-----NQGLILRKEAYEYTGAYGFKDRQDGDHLATQR 293 V L S+ P+ + P+ + G + Y+ TG F D+ A Sbjct: 260 GVALASWH-----PEKLSSPSHEEGSTLDAGELPSLPVYDGTGQQDFADQDAAAAHAQLM 314 Query: 294 MEEFEHAGKFFQAAGNNRNVVPGRWFRLTGHFDVGSQQEREFLIIEAHHSASNNYHVN-- 351 ++ E K F+ AG R + G F L H + + E F ++ H+A NN N Sbjct: 315 LQALEMQNKRFEGAGAARQLAAGARFTLLQH-ERYPEGENAFKVVSVRHAARNNVSANIT 373 Query: 352 -----------NGTAPH----------YENQIKCIRKVIPWRPGRGFNSVEPKIYGLQTA 390 G+A Y N IR +P P ++P G QTA Sbjct: 374 RILAPAAGALFGGSAAAERDDDLQAGTYRNSFTAIRDSVPMVPAAIAERLKPTARGTQTA 433 Query: 391 TVVGPKGEEIHTDEYGRVKVQFHWDR-----------EGELDER---------SSAWLRV 430 V G + TD RVKVQFHW R G LD++ S W+RV Sbjct: 434 LVTGAADAAVTTDRNHRVKVQFHWQRGKSPNPGGLRETGNLDDKDGNANGNDSSGTWIRV 493 Query: 431 ATAWAGSNFGATFIPRIGCEVIVQFLDGNPDRGLITGMVPNAANMPAWELPANKTQSGI 489 A A +G N+G+ FIPR+G EV+V F++G+ DR ++ + N ++ P + A+ SGI Sbjct: 494 AEAQSGPNWGSQFIPRVGSEVLVDFIEGDIDRPVVVAQLYNGSDAPPFPAGAD---SGI 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,315,911 Number of extensions: 100385 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 12 Length of query: 710 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 618 Effective length of database: 1,184,250 Effective search space: 731866500 Effective search space used: 731866500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)