BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2748.p (328 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 617 e-178 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 112 6e-26 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 101 1e-22 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 89 3e-19 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 82 4e-17 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 617 bits (1591), Expect = e-178, Method: Compositional matrix adjust. Identities = 302/329 (91%), Positives = 313/329 (95%), Gaps = 1/329 (0%) Query: 1 MKSRRFGRTGAEVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 MKSRRFGRTGA+VSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 61 SEQLIAQVLKERGGPRPFVATKAGRRLSPHVASGYQ-RDNLTAFVERSLQNLEVDCLDLV 119 SEQLIAQVLKERGG RPFVATKAGRRLSPHVASGY R L+AF++RSL+NL VDCLDLV Sbjct: 61 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLV 120 Query: 120 QLHCPPTEVYYQQEVFGYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 179 QLHCPPTEVYYQQEVFG++D LVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF Sbjct: 121 QLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 180 Query: 180 RQRPISLFLHEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET 239 RQRPISLFL EAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET Sbjct: 181 RQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET 240 Query: 240 FSGVPYEVALAAVEKIRTLVPPGVSMAQFALRWILMESGVSTVIPGARNVQQAQSNSAAS 299 FSGVPYEVALAAVEKIR LVP GV MAQFALRWILMESG+STVIPGARNV QAQSNSAAS Sbjct: 241 FSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS 300 Query: 300 DLPPLAQEVMQALRELYLHDIAPHVHQRW 328 LPP+ EVMQALR +YLHDIAP+VHQRW Sbjct: 301 ALPPITAEVMQALRAIYLHDIAPYVHQRW 329 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 112 bits (279), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 92/323 (28%), Positives = 149/323 (46%), Gaps = 25/323 (7%) Query: 3 SRRFGRTGAEVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSE 62 +RR G G VS IG G + + ++ A + AL+ G++ +DTAD+YG +E Sbjct: 17 TRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNE 76 Query: 63 QLIAQVLKERGGPRPFVATKAGRRLSPHVASGYQRDNLTAF----VERSLQNLEVDCLDL 118 +L+ + +K R + F+ATK G R P D A+ VE SL+ L V+ +DL Sbjct: 77 ELLGRAIKGRRD-KFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDL 135 Query: 119 VQLH----CPPTEVYYQQEVFGYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQI 174 H P EV G + +LV AGKIR+ G+S +A V ++Q Sbjct: 136 YYQHRIDPATPIEV-----TMGVLADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQS 190 Query: 175 IFNIFRQRPISLFLHEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAF 234 ++++ + P + L + I +A PL G LTG + A DD R+ N Sbjct: 191 EYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSN------ 244 Query: 235 DVGETFSGVPYEVALAAVEKIRTLVPP-GVSMAQFALRWILMESGVSTVIPGARNVQQAQ 293 F G + L EK++ + G + +Q AL W++ + IPG + + + Sbjct: 245 ---PRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLE 301 Query: 294 SNSAASDLPPLAQEVMQALRELY 316 N+ + + LA E +QAL ++ Sbjct: 302 DNAGSVGV-KLAPEDLQALDAIF 323 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 92/317 (29%), Positives = 148/317 (46%), Gaps = 24/317 (7%) Query: 1 MKSRRFGRTGAEVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 M RR G++ +VS + G+ G Q++ + +A + GV+FIDTADVY G+ Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLQESGRIVASAREHGVNFIDTADVYTKGK 57 Query: 61 SEQLIAQVLKERGGPRP--FVATKAGRRLS--PHVASGYQRDNLTAFVERSLQNLEVDCL 116 SE+++ ++L G R +A+K G +S P+ S Y R + E L L+ D L Sbjct: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPN-ESNYSRSWIVRETENILTRLDTDYL 113 Query: 117 DLVQLHCPPTEVYYQQEVFGYMDELVAAGKIRHYGVSV---EKVEEALKAIEYPNVK--- 170 D++ +H + ++ V + +L+ GKIR +GVS ++ E ++ E NV Sbjct: 114 DILYMHRDYQDENLEEAVQA-LGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 Query: 171 SVQIIFNIFRQRPISLFLHEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRH 230 Q +N+ ++P L + V+ P+A G+LTGK A D R Sbjct: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQD-----TRA 227 Query: 231 GEAFDVGETFSGVPYEVALAAVEKIRTLVPPGVSMAQFALRWILMESGVSTVIPGARNVQ 290 G D + E L A + GV + QFA W+L S +S+VI G R + Sbjct: 228 GRG-DKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLA 286 Query: 291 QAQSNSAASDLPPLAQE 307 Q + A++L A+E Sbjct: 287 QMEDYYDAAELKISAEE 303 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 89.4 bits (220), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 101/355 (28%), Positives = 160/355 (45%), Gaps = 53/355 (14%) Query: 1 MKSRRFGRTGAEVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 M+ R GRT +VS I G +WG+ +++ DA A L A++ G++ ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTM----TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVP 56 Query: 57 ----GDGRSEQLIAQVLKERGG-PRPFVATKA-GRRLSPHVA-------SGYQRDNLTAF 103 G +E+ + LK+ G + +ATK G PH + R LT Sbjct: 57 PKAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEA 116 Query: 104 VERSLQNLEVDCLDLVQLHCPPTEVY------YQ----------QEVFGYMDELVAAGKI 147 + SL+ L++D +DL QLH P V YQ +E + E V AGKI Sbjct: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKI 176 Query: 148 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPISLFLHE-AQRRDIAVIAR 201 R G+S E V + L+A E ++ + I N + R + L E A R + ++A Sbjct: 177 RTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAY 236 Query: 202 VPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRTLVPP 261 PLA G+L+GK A F R + + Y VALA Sbjct: 237 SPLAFGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEY-VALARQH-------- 287 Query: 262 GVSMAQFALRWILMESGVSTVIPGARNVQQAQSNSAASDLPPLAQEVMQALRELY 316 G+ AQ AL ++ +++ I GA + Q +SN +++L L ++V+ A+ ++ Sbjct: 288 GLDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAEL-QLGEDVLAAIEAIH 341 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 85/297 (28%), Positives = 133/297 (44%), Gaps = 55/297 (18%) Query: 8 RTGAEVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQ 67 R G V +G G W +G S +A+ AL A LD G+S IDTA++Y +G +E+++A+ Sbjct: 10 RNGQSVPILGQGTWNMGESPAQANAE--VRALQAGLDLGMSLIDTAEMYAEGGAERIVAK 67 Query: 68 VLKERGGPRPFVATKAGRRLSPHVASGYQRDNLTAFVERSLQNLEVDCLDLVQLHCPPTE 127 + R V+ ++ PH AS + A E SL+ L D +DL LH Sbjct: 68 AIAGRRDQVYLVS-----KVLPHNAS---KRGTIAACEASLKRLGTDRIDLYLLHW--RG 117 Query: 128 VYYQQEVFGYMDELVAAGKIRHYGVSVEKVEEALKAIEYPNVKSV---QIIFNIFRQRPI 184 + M+ LV+ GKI +GVS ++ +E P+ + Q+++N+ R+ Sbjct: 118 AHPLAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIE 177 Query: 185 SLFLHEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVP 244 L +AQ+ IAV+A P+ G + +H P Sbjct: 178 YDLLPQAQQAHIAVMAYSPIEQG------------------RILKH-------------P 206 Query: 245 YEVALAAVEKIRTLVPPGVSMAQFALRWILMESGVSTVIPGARNVQQAQSNSAASDL 301 ALA + V+ AQ AL W+L + GV IP A +V Q+N A+ D+ Sbjct: 207 ALTALAQAHR--------VTPAQIALAWVLRQPGV-VAIPKAADVAHVQANRASLDI 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 970,510 Number of extensions: 36653 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 6 Length of query: 328 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 242 Effective length of database: 1,212,660 Effective search space: 293463720 Effective search space used: 293463720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)