BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2746.p (524 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 970 0.0 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 417 e-117 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 216 5e-57 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 142 8e-35 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 126 4e-30 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 970 bits (2507), Expect = 0.0, Method: Compositional matrix adjust. Identities = 500/512 (97%), Positives = 508/512 (99%) Query: 1 MANSPTPPKPYTPPPPLSGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 MA SPTP + YTPPPPL+GANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLGARV 120 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILL ARV Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 Query: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI Sbjct: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 Query: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA Sbjct: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 Query: 241 SPTIVTLGLIALVAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 SPTIVTLG+IAL+AFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI Sbjct: 241 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 Query: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL Sbjct: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 Query: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA Sbjct: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 Query: 421 VGTSISTTAWNNRTILHHAQLTEHATPYSPWLQESVNNISSTLGLNAEQTAGMLERTITF 480 VGTSISTTAWNNR+I+HHAQLTEHATPYSPWLQE+VNN+SSTLGLNAEQTAGMLERTITF Sbjct: 421 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 480 Query: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK 512 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK Sbjct: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSK 512 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 417 bits (1072), Expect = e-117, Method: Compositional matrix adjust. Identities = 225/511 (44%), Positives = 318/511 (62%), Gaps = 14/511 (2%) Query: 17 LSGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISI 76 LSG LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+VITS+A A AI++ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 77 PLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSL 136 PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+ RVLQGL GP++PLSQ+L Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 137 LLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL 196 LL +PK K+ A+ LW MT +VAP+AGP LGG + D Y+WP+IF+IN+PV LI + Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 197 AIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFA 256 + + ET KLPID++GL L+V WVAA Q+MLD+GK+ DWFAS IV L + A+V F Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 257 YFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDS 316 F++WEL +P+VDLR+F R F I + +G FFG++V+ P WLQ Y+GY + S Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 317 GLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILP 376 G +A GI AV+ P++ + +DARK+ + +LR+ + + P Sbjct: 326 GCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWP 385 Query: 377 TLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTIL 436 LQGI +FFVPLT L LS + E +AAGL SF R GA+ TSI T+W N Sbjct: 386 LFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 Query: 437 HHAQLTEHATPYSPWLQESVNNISSTL--GLNAEQTAGMLERTITFQAAMLGINDIFYVS 494 HA+L S + + I + + G++AEQ L +T+ QA ML N IF ++ Sbjct: 446 FHAEL-------SGLVDRGGDAIHTMMASGMSAEQATASLAQTVQNQAVMLSTNHIFMMA 498 Query: 495 AVIFIVIIPLIWII-KPSKGGAGAAEASAAH 524 AV F + +W+ KP++ A+ SAAH Sbjct: 499 AVSFSLAALAVWLAPKPTR----VADTSAAH 525 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 216 bits (549), Expect = 5e-57, Method: Compositional matrix adjust. Identities = 146/485 (30%), Positives = 248/485 (51%), Gaps = 8/485 (1%) Query: 30 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 89 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + LG Sbjct: 23 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 82 Query: 90 RLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 149 L +T+ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 83 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 142 Query: 150 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL---AIYRSRETPT 206 A++G TA++ PV GP +GGW+T++ +W + F+IN+PVG AA +TL AI R Sbjct: 143 NAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVG--AALITLLLVAIPYERPKFE 200 Query: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELNND 266 D G+A L + ++L++G+ WF+SP I L +++ F + ++ Sbjct: 201 QLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRAR 260 Query: 267 HPVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 325 HPV+ LRL R F G + +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 261 HPVIQLRLLRDRQF-GSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGV 319 Query: 326 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTA 385 +++MP + LVD R F+ A ++ + + + + L++G+ T Sbjct: 320 PMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLMRGVGTV 379 Query: 386 MFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRTILHHAQLTEHA 445 + + L + +PP + A+GL S R G++ + + R LH +L E Sbjct: 380 LAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESL 439 Query: 446 TPYSPWLQESVNNISSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAVIFIVIIPLI 505 S +Q S+ LG + ++ TI QA + D+F++ V + + PL+ Sbjct: 440 NANSDLVQSSMTAQGHLLG-GQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLV 498 Query: 506 WIIKP 510 ++P Sbjct: 499 LFLRP 503 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 142 bits (357), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 124/413 (30%), Positives = 194/413 (46%), Gaps = 23/413 (5%) Query: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 V+ FM LD++I N A+P ++ +LG V V+ ++ A P +GWL R G Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 R++ ++LLF L S LC +A +LP+LL ARV+QGL ++P+ + +L A P ++ Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK 208 ALA + + PV GP LGGW+ +S +W WIF IN+PVG++ G L + ++ Sbjct: 178 ALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL-YFLPDDSLAGT 236 Query: 209 LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELNNDH- 267 D +G LL + AF + LD D + T ++ L + A W + H Sbjct: 237 PRFDLLGCVLLSACMVAFSLALDVPFD----SHRTGWSIALFVVAALTVLGYWRHASVHP 292 Query: 268 -PVVDLRLFGRRNFA---GGTIAISIG-YGVFFGNLVILPQWLQQYLGYRSIDSGLSTAP 322 P+ L LFG NF+ G + IG V F ++ LQ LGY + +G+ P Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLL----LQVRLGYSPLHAGMVLLP 348 Query: 323 IGIFAVI----LMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTL 378 I I + PL+ RF D + + +G AI+ + + + V L Sbjct: 349 AAIAGTIAKPWIAPLVKRF--GYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIF 406 Query: 379 LQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 431 G +M F + + L GL + L S V++ +G +I T N Sbjct: 407 --GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 126 bits (317), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 114/421 (27%), Positives = 199/421 (47%), Gaps = 29/421 (6%) Query: 38 LDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVL 97 +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R G++ +F +++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 98 LFVLASWLCGLAPTLPILLGARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTA 157 +F+L S LCG++ TLP + AR LQG+ MVP+ + ++L + K AL+ + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 158 VVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK----LPID- 212 + PV GP LGG+IT + W WIF+INIP+ ++ G+ LA +R P K P+D Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLA---TRFIPDLKAEQVAPLDL 175 Query: 213 ------KIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGLIALVAFAYFVVWELNN- 265 +G +LL+ +A L G+ + T V GL+ L A+ VW Sbjct: 176 KGFLLSALGCSLLMMGLATAGRHLVSGE-----VALTCVLAGLVFLAAY----VWHARRA 226 Query: 266 DHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNL-VILPQWLQQYLGYRSIDSGLSTAPIG 324 P++DL L G S Y V G + +LP LQ GY SG+ T Sbjct: 227 SAPLIDLALLKIPTLRAGVFGGSF-YRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASA 285 Query: 325 IFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPT 384 A+ + + RFL R+V + + W + + Q + +++ L+ G Sbjct: 286 AGAMFMKTIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFR 345 Query: 385 AMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN-NRTILHHAQLTE 443 ++ F L + + + R+ A ++S + +G ++ A + I HA L Sbjct: 346 SLQFTSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVA 405 Query: 444 H 444 Sbjct: 406 Q 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,524,419 Number of extensions: 66596 Number of successful extensions: 412 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 399 Number of HSP's successfully gapped: 16 Length of query: 524 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 434 Effective length of database: 1,193,720 Effective search space: 518074480 Effective search space used: 518074480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)