BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2745.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 669 0.0 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 281 5e-77 gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 113 2e-26 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 77 2e-15 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 669 bits (1726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/408 (93%), Positives = 393/408 (96%), Gaps = 2/408 (0%) Query: 1 MSDSTTQNNPPAKANGNGNGKRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVG 60 MSDSTTQ P +AN NGNGKR RQLILLT+VL+++A ACFLYWFLHARFFEETDDAYVG Sbjct: 1 MSDSTTQQQP--QANNNGNGKRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVG 58 Query: 61 GNVVQISAQVGGTVVAVKADDTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQL 120 GNVVQISAQVGGTVVAVKADDTQ+VK GQ LVALDAADT+LALDQAQAALAQAVRQTRQL Sbjct: 59 GNVVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQL 118 Query: 121 FLNNDTLAANVAAADANLARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQAR 180 FLNNDTLAANVAAAD+NLARAREDLQRRQAGLSSGAVSQEDVSHARDA+KSA AALDQAR Sbjct: 119 FLNNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQAR 178 Query: 181 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA 240 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA Sbjct: 179 AAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIA 238 Query: 241 AGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG 300 AGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG Sbjct: 239 AGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG 298 Query: 301 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRTLEAVP 360 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGR LEAVP Sbjct: 299 AFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVP 358 Query: 361 TNYQTNVYNDLDKQADAIVERIISDNASGLPQARKGKASAAPVMVPHT 408 TNYQTNVY+DL KQADAIV+RIISDNASGLPQA K KA+AAPV VPHT Sbjct: 359 TNYQTNVYDDLGKQADAIVDRIISDNASGLPQAHKSKAAAAPVAVPHT 406 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 281 bits (720), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 158/359 (44%), Positives = 216/359 (60%), Gaps = 28/359 (7%) Query: 21 KRTRQLILLTVVLVIVAAACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKAD 80 KR + + V+ I A YW+L + ETD+AY + ++ + G V AV Sbjct: 31 KRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTATEIATVTPAINGIVAAVDVV 90 Query: 81 DTQLVKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAA------- 133 DTQ VK G LV +D AD +LA+DQA A L + R+ + F N+ LAA V A Sbjct: 91 DTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQKR 150 Query: 134 -------ADANLARAREDLQRRQAGLSSGAVSQEDVSHARDALKSATAALDQARAAAAAN 186 A A+L RA DLQRR+A SG+VS E++S+AR AL +A A L A AA + Sbjct: 151 ASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 210 Query: 187 RA--------------LTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRS 232 RA LT +T+V ++P V+ A + A L+ R + APV G +++R Sbjct: 211 RANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKLDLERTVLRAPVDGVIARRQ 270 Query: 233 VQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVI 292 VQVGQR+ +G L+++VPL+Q+ +DANFKE QL +RIGQPV + AD+YG SV+Y G V Sbjct: 271 VQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVT 330 Query: 293 GFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHG 351 G S GTG AF+++PAQNATGNWIKVVQR+PVRI+LDP+++ PL +GLS +D G Sbjct: 331 GLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSVGLSMVVEIDTRG 389 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 113 bits (283), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 63/140 (45%), Positives = 86/140 (61%), Gaps = 6/140 (4%) Query: 211 AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRI 270 A +N I A V G + + V+VGQ ++ G +M +VP + ++ ANFKE+Q+ +RI Sbjct: 236 ASVNLGYTEIRASVDGRIGDKQVRVGQFVSPGTRMMTVVPT-RFYVSANFKETQIGIMRI 294 Query: 271 GQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALD-- 328 GQP + D V++ G V S GTG FSLLP QNATGN+ K+VQRVPVR+A+D Sbjct: 295 GQPAIIKVDAL-PGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPVRVAIDAT 353 Query: 329 PEQVRAHPLRIGLSTTATVD 348 PE+ RA GLS T V+ Sbjct: 354 PEETRA--FVAGLSVTVEVN 371 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 99/340 (29%), Positives = 153/340 (45%), Gaps = 43/340 (12%) Query: 27 ILLTVVLVIVAAACFLYWFLHARFFEE--TDDAYVGGNVVQISAQVGGTVVAVKADDTQL 84 +L+T + ++A L W L + T D V VV I+ V G VVA+ D QL Sbjct: 9 VLITTAIFLLA--ILLAWSLWQHYMHSPWTRDGRVRAEVVNIAPDVSGQVVALPVRDNQL 66 Query: 85 VKLGQPLVALDAADTKLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAADANLARARED 144 VK G L+ +D A LAL QA+AA V A A L R + Sbjct: 67 VKKGDLLMEIDPARYTLALQQAEAA---------------------VTARKAELDVRRAE 105 Query: 145 LQRRQAGLSSGAVSQEDVSHARDALKSATAALDQA--RAAAAANRALTDHTSVTEHPNVL 202 ++ R+A L+ + DAL + A + A + A+ + + Sbjct: 106 VEARRADLNMRRAQARRRADV-DALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQY 164 Query: 203 QAATAVRN-AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFK 261 QAA + R+ A LN R + +PV G+++ +V+ G AAG +A++ W+ A F+ Sbjct: 165 QAALSQRDTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYFE 224 Query: 262 ESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG-----AFSLLPAQNATGNWIK 316 E++L + IG I + G+S+ +G V + G + L N T NW++ Sbjct: 225 ETKLPLLSIGDRAR-IQLINGASM--EGHVDSIARGIYDRDNPQSRELTADVNPTFNWVR 281 Query: 317 VVQRVPVRIALD--PEQVRAHPLRIGLSTTATVDIHGDGR 354 + QR+PVRI LD PE V L GL+ T ++ G G+ Sbjct: 282 LAQRIPVRIQLDKVPENVL---LASGLTCTVVLE-PGSGK 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 950,210 Number of extensions: 35452 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 7 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)