BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2702.p (1396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311969|ref|YP_003776061.1| cobalamin biosynthesis protein ... 2310 0.0 >gi|300311969|ref|YP_003776061.1| cobalamin biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 1397 Score = 2310 bits (5987), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1192/1409 (84%), Positives = 1259/1409 (89%), Gaps = 25/1409 (1%) Query: 1 MSTLRRRPAMSDALALARIVLLTHAPTDLAMLAAARGQLPVDFPPVAGYDLQGLQAHTDE 60 MS RR AMS+ LARI LLTHAPTDLAMLA AR QLP DFP VAGYDLQ LQA + Sbjct: 1 MSIPNRRAAMSE--PLARIALLTHAPTDLAMLATARAQLPADFPAVAGYDLQRLQAADQD 58 Query: 61 DGQPLHTLAALLRTELAGAQVIVLRILGRLGAVSGFPELLRLARAQGRQLIVLSGTGEPD 120 +LAALLR ELA AQVIVLR+LGRLGAV GFPE+LR+ RA+GRQLIVLSGTGEPD Sbjct: 59 QASQWPSLAALLRGELAAAQVIVLRVLGRLGAVQGFPEMLRVVRAEGRQLIVLSGTGEPD 118 Query: 121 AELAAASTAPADVQQQALAYFNGGGSVNLAQLLRCLSDRLLLSGYGYEPAQALPEHGLYH 180 ELAAASTAPADVQQQALAYFNGGGSVNLAQ+LRCLSDR+LLSGYGYEPAQALPEHGLYH Sbjct: 119 PELAAASTAPADVQQQALAYFNGGGSVNLAQMLRCLSDRMLLSGYGYEPAQALPEHGLYH 178 Query: 181 PDLEQGAGVADWLALRAQAPSGKPAVGIIFYRAHWLSGNTGFIDALVAALEARGMDVLPV 240 PDLEQ AG+ADWLALRAQAP+GKPAVGIIFYRAHWLSGNT FIDALVAALEARG+DVLPV Sbjct: 179 PDLEQDAGIADWLALRAQAPAGKPAVGIIFYRAHWLSGNTAFIDALVAALEARGLDVLPV 238 Query: 241 FTSSLRVSAAGAQALPPALAYFATGQVDLILNTTSFAMGEITSGGVTPAGWSVSVLEQLD 300 FTSSLRVSA G QALPPAL YF GQVDLI+NTTSFAMGEIT+GGVTPAGWSVSVLEQLD Sbjct: 239 FTSSLRVSAGGGQALPPALQYFGAGQVDLIINTTSFAMGEITAGGVTPAGWSVSVLEQLD 298 Query: 301 IPVLQAITSGMSQAQWQHSTRGMNPLDAAMNVVLPEFDGRIIGVPLSFKQSTPTGAVHYQ 360 +PVLQAITSGM+QAQWQHSTRGMNPLDAAMNVVLPEFDGRIIGVPLSFK++T +GAV YQ Sbjct: 299 LPVLQAITSGMTQAQWQHSTRGMNPLDAAMNVVLPEFDGRIIGVPLSFKETTDSGAVQYQ 358 Query: 361 PLADRVRRIAGLTQRLVRLRRTPNAQKRIAFIFTNSNSKAAQIGNAVGLDAPESLMAVLR 420 PL DRVRRIAGL QRLVRLR TPNA KRIAFIFTNS+SKA+QIGNAVGLDAPESLMAVLR Sbjct: 359 PLPDRVRRIAGLAQRLVRLRSTPNANKRIAFIFTNSSSKASQIGNAVGLDAPESLMAVLR 418 Query: 421 ALAAEGYAIDELPASGTALIHELVDRCSYDDILLTEDQLRRAAARIPAQRYAQWFAALPP 480 AL+A+GYAID LP +GTALIHELVDRCSYDDILLTEDQLRRAAARIPA RYAQWFAALP Sbjct: 419 ALSADGYAIDALPQTGTALIHELVDRCSYDDILLTEDQLRRAAARIPAARYAQWFAALPT 478 Query: 481 ALQARMQRQWGAAPGQAYVHDQHLVIAGLDLGNAFVVLQPPRGYGMDPDAIYHQPDLPPT 540 +LQ +MQRQWGAAPGQAYVHDQHLVIAGLDLGNAFV LQPPRGYGMDPDAIYHQPDLPPT Sbjct: 479 SLQEKMQRQWGAAPGQAYVHDQHLVIAGLDLGNAFVALQPPRGYGMDPDAIYHQPDLPPT 538 Query: 541 HHYYALYRWLDEEWGADAIVHVGKHGTLEWLPGKGVGLSEECFPDALLGDVPLFYPFIIN 600 HHYYALYRWLDEEWGA AIVHVGKHGTLEWLPGKGVGLS+ECFPDALLGD+PLFYPFIIN Sbjct: 539 HHYYALYRWLDEEWGAHAIVHVGKHGTLEWLPGKGVGLSDECFPDALLGDMPLFYPFIIN 598 Query: 601 DPGEGSQAKRRAHAVVVDHLTPPMTTADSYGALAQLTQLVDEYYQVEVLDPSKLPLLQQQ 660 DPGEGSQAKRRAHAVVVDHLTPPMTTADSYGALAQLTQLVDEYYQVEVLDPSKLPLLQQQ Sbjct: 599 DPGEGSQAKRRAHAVVVDHLTPPMTTADSYGALAQLTQLVDEYYQVEVLDPSKLPLLQQQ 658 Query: 661 IWELVRQTNLDTDLQFKLL-------------HHDHDHDHDHGHHHHGHHHHHDHGAHDH 707 IWELVRQTNLDTDLQ+KLL H H H H H HHH H AH Sbjct: 659 IWELVRQTNLDTDLQYKLLHHDHDHDHDHDHDHGHDHDHHHHHHGHAHHHHDHGDHAHHD 718 Query: 708 AHHDHAHDADQLPHALTELDGAGVAHLIEDLDGYLCELGAAQIRDGLHILGRTPDSQQMP 767 HDHAH+ +LPHALTELDGAGVAHLIEDLDGYLCELGAAQIRDGLHILGRTPD QMP Sbjct: 719 HDHDHAHEEGELPHALTELDGAGVAHLIEDLDGYLCELGAAQIRDGLHILGRTPDQAQMP 778 Query: 768 DMLVALTRLPNQDLPGLQEELAKLFGLSLDMLLEHKGRRLNIDDTLARVAGRAIVTRADA 827 DMLVALTRLPNQDLPGLQEE+A+LFGLSLDMLLEHKGRRLNIDD LAR A R I TRADA Sbjct: 779 DMLVALTRLPNQDLPGLQEEVARLFGLSLDMLLEHKGRRLNIDDALARCADRPIATRADA 838 Query: 828 LEAIDALCLALMQALQQHGFAVTAIDDVLQSVLGSLQNTPAEQQPRLQPALAKGPGSVLG 887 LEAIDALCL LMQALQ+H FA TAID VL SV +L+ EQ+PRLQPA+++ SVLG Sbjct: 839 LEAIDALCLRLMQALQEHDFAPTAIDRVLGSVFAALEQASPEQRPRLQPAMSQAATSVLG 898 Query: 888 AMRSLSRPAAAPAAAPAAAPAAAPVKRTAAQIQTKTSDTGALRRVLVFTCEQLVPRLLRT 947 MR+++ A A A+ + +DTGA+RRVL F CE+LVPRL RT Sbjct: 899 GMRAMA----------RPAAPAPRRAAPASAPSRRAADTGAVRRVLAFACEELVPRLHRT 948 Query: 948 EEEISNLLHGLRGGYIPAGPSGSPTRGMAHILPTGRNFYSVDPRSVPSQSAWRVGQQLAL 1007 EEI NLL+GLRGGYIPAGPSGSPTRGMAHILPTGRNFYSVDPRSVPSQSAWRVGQQLA Sbjct: 949 SEEIGNLLNGLRGGYIPAGPSGSPTRGMAHILPTGRNFYSVDPRSVPSQSAWRVGQQLAR 1008 Query: 1008 EVLQRHHRESGDYPESVAISIWGTSAMRTHGDDVAQILALMGARPIWREGNRQVAGVELV 1067 EVLQRHHRE+G YPESVAISIWGTSAMRTHGDDVAQIL LMGARP+WR GNRQVAGVELV Sbjct: 1009 EVLQRHHRETGHYPESVAISIWGTSAMRTHGDDVAQILWLMGARPLWRAGNRQVAGVELV 1068 Query: 1068 PLAQLQRPRIDVTTRISGFFRDAFPQLIELIDDAVQLAIAADEPEHMNFVRKHYLRDLQA 1127 PL +LQRPRIDVTTRISGFFRDAFPQLIELIDDAVQLAIAADEPE MNFVRKHYLRDL Sbjct: 1069 PLEELQRPRIDVTTRISGFFRDAFPQLIELIDDAVQLAIAADEPETMNFVRKHYLRDLAQ 1128 Query: 1128 AVTSGMGQAEAAAQAAYRVFGAKPGSYGAGILPLIQEKNWQDQADFAEAYINWGGYAYGR 1187 + +G+GQAEAAAQA+YRVFGAKPGSYGAGILPLIQEKNWQDQADFAEAYINWGG+AYGR Sbjct: 1129 GMGAGLGQAEAAAQASYRVFGAKPGSYGAGILPLIQEKNWQDQADFAEAYINWGGFAYGR 1188 Query: 1188 KVQGADQREAFRQRLSGVQVALHNQDNREHDIFDSDDYLQYHGGMIATIRALSGQQPRHY 1247 KVQGADQR+AFRQRL+GVQVALHNQDN EHDIFDSDDYLQYHGGMIATIRALSGQQPRHY Sbjct: 1189 KVQGADQRDAFRQRLAGVQVALHNQDNHEHDIFDSDDYLQYHGGMIATIRALSGQQPRHY 1248 Query: 1248 FGDSHDPQRAAVRDLKEETLRVFRARVVNPKWLASIRRHGYKGGLELTATVDYLFGYDAT 1307 FGDSHDPQRAAVRDLKEETLRVFRARVVNPKWLASI+RHGYKGGLEL+ATVDYLFGYDAT Sbjct: 1249 FGDSHDPQRAAVRDLKEETLRVFRARVVNPKWLASIQRHGYKGGLELSATVDYLFGYDAT 1308 Query: 1308 AQVLEDWMYQQLAETYGFDPEMRAFLEQANPWAQNAIAERLLEAASRGMWQTPPAQTIQQ 1367 AQVL+DWMYQQLAETYGFDP MR FLE+ANPWAQNAIAERLLEAASRGMWQ P AQT+++ Sbjct: 1309 AQVLDDWMYQQLAETYGFDPAMRQFLEEANPWAQNAIAERLLEAASRGMWQAPDAQTLER 1368 Query: 1368 LRTLYLHSEALLEARGETVRGSEHTKDMQ 1396 LR LYLHSE LLEARGETVRGSEHTKD+Q Sbjct: 1369 LRQLYLHSETLLEARGETVRGSEHTKDLQ 1397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 4,514,095 Number of extensions: 202106 Number of successful extensions: 668 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 667 Number of HSP's successfully gapped: 1 Length of query: 1396 Length of database: 1,619,870 Length adjustment: 97 Effective length of query: 1299 Effective length of database: 1,160,575 Effective search space: 1507586925 Effective search space used: 1507586925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 137 (57.4 bits)