BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2666.p (508 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 229 3e-61 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 70 3e-13 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 67 5e-12 gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 59 1e-09 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 229 bits (585), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 161/493 (32%), Positives = 242/493 (49%), Gaps = 13/493 (2%) Query: 7 ILAIDAGTSGTRAAVVTANGQVSCLHHLPLPIHSPQPGVVEQDAALIWQHTLAVCRATLQ 66 +LA+D GTS +R+ + +G + P PQPG VE D +W L + L Sbjct: 4 LLALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLA 63 Query: 67 QARQKRLQVRAIGLCNQRASAVLWDTVSGQPLAPALVWQDSRHAAQLAKLAVHWERRLRP 126 Q+ K ++ A+G+ NQR + V+W+ +G+P+ A+VWQD R A L ER L Sbjct: 64 QSGIKASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALR---ERGLGE 120 Query: 127 RLGRPLG-PRSPYLWAAHL---LRSNPAVAQAWRQRRLGFGSIDTWLLWQLSAGRLLATS 182 + + G PY L L + + L FG++D+WL+W L+ GRL + Sbjct: 121 AIQKKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTVDSWLVWNLTGGRLHVSD 180 Query: 183 ATNATACNAYCLDEHAYALDWLQALDFPLELLPPLYQDADDYGMSETSVLGAALPIRACA 242 +NA+ + + A+ + LQ L P +LP + A YG + LGAA+ I A Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEIDAQHLGAAVKIGGIA 240 Query: 243 GDQLAGAIGLGCLAAGQALCVHGTGSFINLLVGTRAPLATGISEQTGSVVVTARRQQGVS 302 GDQ + G C+ G A +GTG F+ L G + + T + V ++Q Sbjct: 241 GDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQKQ---- 296 Query: 303 HFALESFCATTGAVLTWACQRQRWFPHARALLSLAASVPHAGGVSFVPALAGIQAPQRHS 362 +ALE GAV+ W + + + LA SV +GGV FVP+ G+ AP + Sbjct: 297 -YALEGSVFIGGAVVQWLRDGLKAIKTSTDVEGLACSVEDSGGVVFVPSFTGLGAPYWNP 355 Query: 363 GARAALQGLSLASSRAEIAHALVEGIAQAVAACVHANAEVAGLPVTQLMSGGGLSASDLL 422 A+ A+ GLS S+ A IA A +E IA A ++A A P+T+L GG +A++LL Sbjct: 356 SAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGGAAANNLL 415 Query: 423 LQLQADLTGVPTYRTHQAAHASLRGIAYLAGSQGLLWDDLPQACARTRLRAVFEPRLDAA 482 LQ QADL G+P R Q + G AYLAG ++ DL + ++ R+ FEP + Sbjct: 416 LQFQADLLGIPVIRP-QVTETTALGAAYLAGIATGVYRDLSELSSQWRMEHTFEPSIGRD 474 Query: 483 ARTARRAPWQARV 495 AR W+A + Sbjct: 475 QAQARIHAWEAAI 487 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 70.5 bits (171), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 105/451 (23%), Positives = 181/451 (40%), Gaps = 56/451 (12%) Query: 7 ILAIDAGTSGTRAAVVTANGQVSCLHHLPLPIHSPQPGVVEQDAALIWQHTLAVCRATLQ 66 +L +D GT+ T++ V T +G+V H P+ +PG+ EQD I LA ++ Sbjct: 10 MLGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVK 69 Query: 67 QARQKRLQVRAIGLCNQRASAVLWDTVSGQPLAPALVWQDSRHAAQLAKLA-VHWERRLR 125 A+ ++ + S + D + L+ ++ W D R + ++ H + Sbjct: 70 AAKAAPQEIALLSFSAAMHSVIALDA-ENRLLSNSITWGDIRASVWAERIKHEHDGNAIY 128 Query: 126 PRLGRPLGPRSP---YLWAAHLLRSNPAVAQAWRQRRLGFGSIDTWLLWQLSAGRLLATS 182 R G P+ P SP +W H P V R F I +LL+QL ++ S Sbjct: 129 RRTGTPVHPMSPLCKLMWMRH---EKPDVF----HRAARFVGIKEYLLYQLFGQWVVDHS 181 Query: 183 ATNATAC---NAYCLDEHAYALDWLQALDFPLEL-----LPPLYQDADDYGMSETSVLGA 234 +AT D+ A AL ++ P + LP L + M++ L Sbjct: 182 IASATGMFNLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSE-----MAQRLGLSV 236 Query: 235 ALPIRACAGDQLAGAIGLGCLAAGQALCVHGTGSFINLLVGTRAPLATGISE----QTGS 290 P A D + +G+ + G + + +GT + T I E G Sbjct: 237 DTPFIIGANDGVLSNLGVNAIEIGH----------VAVTIGTSGAMRTVIDEPRTDPQGR 286 Query: 291 VVVTARRQQGVSHFALESFCATTGAVLTWACQRQRWF-----------PHARALLSLAAS 339 + A ++ H+ + G + W P+ AL +A Sbjct: 287 LFCYALTEK---HWVVGGPVNNGGNIFRWVRDELATAEAAAAREEGVDPY-EALTRIAEK 342 Query: 340 V-PHAGGVSFVPALAGIQAPQRHSGARAALQGLSLASSRAEIAHALVEGIAQAVAACVHA 398 V P A G+ F P +AG +AP ++ R + GL+L + + A +EG+ + + + A Sbjct: 343 VRPGAEGLLFHPFMAGERAPLWNADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPA 402 Query: 399 NAEVAGLPVTQLMSGGGLSASDLLLQLQADL 429 E+ G P ++M+ GG + S L Q+ AD+ Sbjct: 403 LEELVG-PTKRMMATGGFARSALWRQMMADI 432 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 66.6 bits (161), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 121/468 (25%), Positives = 172/468 (36%), Gaps = 85/468 (18%) Query: 6 AILAIDAGTSGTRAAVVTANGQVSCLHHLPLPIHSPQPGVVEQDAALIWQHTLAVCRATL 65 + L ID GTS + + + V L + +P P EQ W TL A Sbjct: 2 SYLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALR 61 Query: 66 QQARQKRLQVRAIGLCNQRASAVLWDTVSGQPLAPALVWQDSRHAAQLAKLAVHWERRLR 125 QA Q +R IGL Q A L D +G L PA++W D R A+ +L L Sbjct: 62 TQAPQAFQALRGIGLSGQMHGATLLDR-NGNVLRPAILWNDMRAHAECVELEA-----LV 115 Query: 126 PRLGRPLGPRS-PYLWAAHLL---RSNPAVAQAWRQRRLGFGSIDTWLLWQLSAGRLLAT 181 P G R+ P A LL + PAV +A ID LL + G L Sbjct: 116 PDAVAITGNRAMPGFTAPKLLWLSKYEPAVYRA----------IDKVLLPKDFLGWKLTG 165 Query: 182 SATNATACNAYCLDEHAYALDW----LQALDFPLELLPPLYQDADDYGMSETSV-----L 232 + + A L DW L A +P L + + G + + Sbjct: 166 EFVSEMSDAAGTLWLDVARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGI 225 Query: 233 GAALPIRACAGDQLAGAIGLGCLAAGQALCVHGTGSFINLLVGTRAPLATGISEQTGSVV 292 + + AGD A A+G+G + AG A G+ + AP A Sbjct: 226 SGPVVVAGGAGDNAASAVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNA----------- 274 Query: 293 VTARRQQGVSHFALESFCATTGAVLTWACQRQRWFPHARALLSLAASVPHAGGVS----- 347 QQGV +FC C +W +LS AAS+ GV+ Sbjct: 275 -----QQGV-----HAFCH---------CLPGQWH-QMSVILSAAASLHWLSGVTARPVA 314 Query: 348 -------------------FVPALAGIQAPQRHSGARAALQGLSLASSRAEIAHALVEGI 388 F+P L G + P + A+ L G++ A A +A+A++EG+ Sbjct: 315 ELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAHESAHLAYAVMEGV 374 Query: 389 AQAVAACVHANAEVAGLPVTQLMSGGGLSASDLLLQLQADLTGVPTYR 436 A A+A +A + AG + GG S S L A G+P R Sbjct: 375 AFAMADG-YAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRR 421 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 42/110 (38%), Positives = 55/110 (50%), Gaps = 2/110 (1%) Query: 8 LAIDAGTSGTRAAVVTANGQVSCLHHLPLPIHSPQPGVVEQDAALIWQHTL-AVCRATLQ 66 L ID GTS +A V+ A G + L L + P P EQ A WQ T V + Q Sbjct: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLRTQ 62 Query: 67 QARQKRLQVRAIGLCNQRASAVLWDTVSGQPLAPALVWQDSRHAAQLAKL 116 ++ +RAIGL Q AVL D + L PA++W DSR A + A+L Sbjct: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAEL 111 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,407,371 Number of extensions: 51297 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 4 Length of query: 508 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 419 Effective length of database: 1,198,455 Effective search space: 502152645 Effective search space used: 502152645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)