BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2665.p (505 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 67 4e-12 gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 61 3e-10 gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 57 4e-09 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 66.6 bits (161), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 80/281 (28%), Positives = 121/281 (43%), Gaps = 30/281 (10%) Query: 53 LLLGGLFLEALSHSALSVGL--PAMIGQLGLGPPEVAWLASSAAASALLANPLGGWLADR 110 ++ GL A + LS+G P + GL P+ + LL + G LADR Sbjct: 25 FVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADR 84 Query: 111 WGRRRPLLLAKLLALGGAVLVACAPDFASILMGRLLAGIAFGLDFAVAMAMLAEITPQHL 170 GRR L+ A ++ V A APDF +L RLL GI G V M+AE P Sbjct: 85 IGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSAR 144 Query: 171 RSR-LSLWQALWYLAVCGNLLLVLLVEPWDVGQSLWRYPLAATALAAFLMLNLQLLFLVE 229 R R L + ++ W + +L L+ W G WR T L A + + L+ ++ E Sbjct: 145 RGRWLVMLESFWAIGTICLAVLALVAVSW--GNDAWRVIFFVTGLPALVGVVLR-FYVPE 201 Query: 230 SPCWLARRRRWQECAEALSRLYDA-------PYFQ--LLERHPAQHNGGLTKAPHASLRQ 280 SP +L R + + L R+ P Q ++ER P ++ A+LR+ Sbjct: 202 SPMYLNRSGKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKP------MSALFSAALRR 255 Query: 281 LLHQPYLLRLILAATVQVVQAVQYFAIGWYLPI-LGLELFG 320 YL ++ ++ Y+ + YLP+ LG E FG Sbjct: 256 RSLSLYLAWGLI--------SIAYYGVFVYLPVKLGSEGFG 288 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 60.8 bits (146), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 77/303 (25%), Positives = 130/303 (42%), Gaps = 26/303 (8%) Query: 78 QLGLGPPEVAWLASSAAASALLANPLGGWLADRWGRRRPLLLAKLLALGGAVLVACAPDF 137 LGL +V W S A A++ + G L DR GR++ ++ L + + A A DF Sbjct: 55 TLGLDAAQVGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDF 114 Query: 138 ASILMGRLLAGIAFGLDFAVAMAMLAEITPQHLRSRLSLW--QALWYLAVCGNLLLVLLV 195 + RL G+ G ++A + + E+ P +R R++L + W A G L ++++ Sbjct: 115 LWFALCRLATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVIL 174 Query: 196 EPWDVGQSL-WR--YPLAATALAAFLMLNLQLLFLVESPCWLARRRRWQECAEALSRLYD 252 + +G + WR + L A A +++ + ESP WLA AEA+ R + Sbjct: 175 DERVLGPVMGWRVCFLLGAVLAVAIVLVRRH---VPESPRWLAMHGH-HARAEAVVREIE 230 Query: 253 APYFQLLERH------PAQHNGGLTKAPHAS--------LRQLLHQPYLLRLILAATVQV 298 A Q++ H P G +++P + + Q+L Y R ++ A + V Sbjct: 231 A---QVMAEHGTLPALPTAAPGESSRSPGQAVGLLSWREIAQVLLHHYRARSLMVALMMV 287 Query: 299 VQAVQYFAIGWYLPILGLELFGEDARQAAWGALVFNLCGIAGGLLAPWLARRLGLRNAAA 358 QA Y AI + ++ F + A F L + G LL L +G R A Sbjct: 288 AQAFVYNAIFFTYSLVLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIA 347 Query: 359 CGF 361 + Sbjct: 348 ATY 350 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 47/165 (28%), Positives = 82/165 (49%), Gaps = 24/165 (14%) Query: 95 ASALLANPLGGWL----ADRWGRRRPLLLAKLLALGGAVLVACAPDFASI--------LM 142 A+ L P+GGWL AD+ GR+ +L++ L+ GG++ VA P +A+I L+ Sbjct: 69 AAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAWAPALLLL 128 Query: 143 GRLLAGIAFGLDFAVAMAMLAEITPQHLRSRLSLWQALWYLAVCGNLLLVLLV----EPW 198 RL G++ G ++ + ++E+ P R + +Q Y+ + G LL +LV + W Sbjct: 129 ARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQ---YVTLIGGQLLAVLVLFGMQQW 185 Query: 199 DVGQSL----WRYPLAATALAAFLMLNLQLLFLVESPCWLARRRR 239 L WR P A+ A + + L+ L E+ AR+++ Sbjct: 186 LTKAELMAWGWRVPFVLGAVGALVAMYLRSS-LAETSSAGARKKK 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,309,826 Number of extensions: 44288 Number of successful extensions: 403 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 412 Number of HSP's successfully gapped: 10 Length of query: 505 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 416 Effective length of database: 1,198,455 Effective search space: 498557280 Effective search space used: 498557280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)