BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2656.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 818 0.0 gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 394 e-110 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 258 9e-70 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 818 bits (2113), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/441 (97%), Positives = 439/441 (99%) Query: 1 MSAQKPPLYKSLYFQVLCAIVIGILLGHFYPSTGEAMKPLGEGFVKLIKMIIAPVIFCTV 60 M+AQKPPLYKSLYFQVL AIVIGILLGHFYPSTGEAMKPLG+GFVKLIKMIIAPVIFCTV Sbjct: 1 MTAQKPPLYKSLYFQVLVAIVIGILLGHFYPSTGEAMKPLGDGFVKLIKMIIAPVIFCTV 60 Query: 61 VIGIAGMEDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPSTLDTKSI 120 VIGIAGMEDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDP++LDTKSI Sbjct: 61 VIGIAGMEDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPASLDTKSI 120 Query: 121 ASYTAPGKMGSVTDFIMGIIPTSMFDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDF 180 A+YTAPGKMGSVTDF++GIIPTSM DAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDF Sbjct: 121 AAYTAPGKMGSVTDFVLGIIPTSMVDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDF 180 Query: 181 IEKISHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVV 240 IEKISHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVV Sbjct: 181 IEKISHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVV 240 Query: 241 LGIITRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTG 300 LGIITRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTG Sbjct: 241 LGIITRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTG 300 Query: 301 YSFNLDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAAT 360 YSFNLDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAAT Sbjct: 301 YSFNLDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAAT 360 Query: 361 LSAVGHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNE 420 LSAVGHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNE Sbjct: 361 LSAVGHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNE 420 Query: 421 TVEDAQAPEAVLDRTEAKMHH 441 TVEDAQAPE++LDRT+AKMHH Sbjct: 421 TVEDAQAPESILDRTDAKMHH 441 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 394 bits (1011), Expect = e-110, Method: Compositional matrix adjust. Identities = 213/418 (50%), Positives = 296/418 (70%), Gaps = 3/418 (0%) Query: 8 LYKSLYFQVLCAIVIGILLGHFYPSTGEAMKPLGEGFVKLIKMIIAPVIFCTVVIGIAGM 67 L+KSL+ QV+ A++ GI++G F+P G+ +KPLG+GF+KLIKMII ++FC VV GI G Sbjct: 5 LFKSLFGQVVLALIGGIIIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGICGA 64 Query: 68 EDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPSTLDTKSIASYTA-P 126 D+KKVG G A++YFEVV+T+AL++GL+L +QPG GMN+DPS LD S++ Y A Sbjct: 65 SDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYMANA 124 Query: 127 GKMGSV--TDFIMGIIPTSMFDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKI 184 GK+ +FIM +IP + AF GDVLQVLL++V FG AL G +G V + + Sbjct: 125 GKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALVASL 184 Query: 185 SHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGII 244 S F + +++AP+G GA++FT+GKYG+GSL LA L+ FY + F+ VVLG I Sbjct: 185 SDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVLGGI 244 Query: 245 TRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFN 304 R G SI+K ++Y++EEL++VL T+SS+SVLP+++ K+E+ G KK+ VGLVIPTGYSFN Sbjct: 245 LRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGYSFN 304 Query: 305 LDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAV 364 LD +IYLTMAA+FIAQATNT ++ +L + L+TSKGA G+ GS ++LAATL+ + Sbjct: 305 LDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATLTTI 364 Query: 365 GHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETV 422 +PV GL L+L ID F+ ARAL N +GN VAT+V+A W ++D R VLN E+V Sbjct: 365 PAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWERDIDKVRARAVLNGESV 422 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 258 bits (658), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 154/421 (36%), Positives = 247/421 (58%), Gaps = 15/421 (3%) Query: 16 VLCAIVIGILLGHF---YPSTGEAMKPLG------EGFVKLIKMIIAPVIFCTVVIGIAG 66 + A+++GIL+G+ + +A + G + F++LIKM+IAP++F T+V+GIA Sbjct: 11 IFAAMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLVFSTLVVGIAH 70 Query: 67 MEDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPSTLDTKSIASYTAP 126 M D K VG+ G +L +F + S V+L +G+++ N LQPG G+ + PS + + A Sbjct: 71 MGDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVAL-PSP---DAAGAGLAT 126 Query: 127 GKMGSVTDFIMGIIPTSMFDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKISH 186 K +V +F ++P S+ +A A+ +VLQV++ ++ FG AL G RG + I+ +SH Sbjct: 127 SKF-TVKEFFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVIDDLSH 185 Query: 187 VLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGIITR 246 + + +MK AP+ F AM+ T+ G+ L S A M FY + +L +++ + Sbjct: 186 TMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFYFSLVLLWVILIAVGFI 245 Query: 247 LHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFNLD 306 I+ + IKE L+ T+SSE+ P++L ++ G K+ + V+P GYSFNLD Sbjct: 246 FLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGYSFNLD 305 Query: 307 GTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAVGH 366 G+ IY T A +FIAQA M ++T++ VL+LTSKG AG+ + +V+AATL + Sbjct: 306 GSMIYCTFATLFIAQAYGIHMPISTQITMMLVLMLTSKGIAGVPRASLVVIAATLHQF-N 364 Query: 367 VPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETVEDAQ 426 +P AGL +ILGID F+ R+ TN +GN +A+ VVAKW G L +E E ++A+ Sbjct: 365 IPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWEGGLMTEEEAARAEVEIEQEAE 424 Query: 427 A 427 A Sbjct: 425 A 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.141 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,277,088 Number of extensions: 51646 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 3 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)