BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2645.p (699 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 1217 0.0 gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 77 6e-15 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 1217 bits (3148), Expect = 0.0, Method: Compositional matrix adjust. Identities = 590/696 (84%), Positives = 635/696 (91%) Query: 4 MKRNPYGRRNFLKAAARSGGAALALSSLPASIRKALAIAPSSPTGSIKDVKHVVILMQEN 63 MKR RR+FLK+AAR+G AALAL++LPASIRKALA+AP+SPTG+IKDVKHVVILMQEN Sbjct: 1 MKRKSQDRRHFLKSAARTGSAALALTALPASIRKALAVAPASPTGTIKDVKHVVILMQEN 60 Query: 64 RSFDHYFGCLKGVRGFGDSIPRPLPDGNPVFYQRDAQGKLVLPYRLEMATTSAGRAGGNL 123 RSFDHYFGCL+GVRGF D IPRPLPDG+PVF+QRD QG+LVLPYR+EMATTSAGRAGGNL Sbjct: 61 RSFDHYFGCLRGVRGFADPIPRPLPDGSPVFHQRDGQGRLVLPYRMEMATTSAGRAGGNL 120 Query: 124 PHAWKDQHAAWNRGDYDRWIEAKKDTVTMGYFTREDLPFHYALAEAFTICDAYHCSVLGS 183 PHAWKDQHAAWNRGDYD+WIEAKKDTVTM YFTREDLPFHYALA+AFT+CDAYHCSVLGS Sbjct: 121 PHAWKDQHAAWNRGDYDKWIEAKKDTVTMAYFTREDLPFHYALADAFTLCDAYHCSVLGS 180 Query: 184 TNPNRFHLMSGGIDLAGRFGGPMTYQPRTGAWSGVPGQVRTGADSFRWMTYPERLEQHQV 243 TNPNRFHLMSGGIDL GR GGPMT+QPRTG+ SGVPG+ R+GADSF W TYPERLEQH V Sbjct: 181 TNPNRFHLMSGGIDLQGRAGGPMTFQPRTGSCSGVPGERRSGADSFGWTTYPERLEQHGV 240 Query: 244 SWKVYQGTDEEQQDHGDYPSDFNVLQYFFNQYQTAAPDSPLWQKACSKFTLADLARDVQG 303 SWKVYQGT+E +DHGDYPSDFNVLQYFF QYQ AAPDSPLWQKACSKFTLADLARDV Sbjct: 241 SWKVYQGTEELHKDHGDYPSDFNVLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDVNS 300 Query: 304 GSLPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF 363 G LPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF Sbjct: 301 GKLPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF 360 Query: 364 DHVVPPTPPLHAQQGLSTVDVSGEFHRVGDYVNPADDLPAGLGARVPMLVISPWSKGGYV 423 DHVVPPTPPLHA QGLSTV+ S EFHR GDYVNPADDLPAGLGARVPM+VISPWSKGGYV Sbjct: 361 DHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPWSKGGYV 420 Query: 424 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLSTAFDFSRRDALTPVLPDTSAYRA 483 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDL++AFDFSRRDA +PVLPDTSAYRA Sbjct: 421 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVLPDTSAYRA 480 Query: 484 LSDAQASLPVPVAPSNPVMPQQERGARLARALPYDLAVHDRVLPAQGSIQLDFLNPGRAG 543 LSDAQA LP PVAPS+P MPQQERG R ARALPY LAV DRV+P Q ++QLDFLNPG+AG Sbjct: 481 LSDAQARLPAPVAPSSPAMPQQERGMRPARALPYVLAVDDRVVPDQDALQLDFLNPGKAG 540 Query: 544 VVYQVYARQDSERYKTFTVEPGKSLSAQWSGVGPVGGYELKVYGPNGFLRELRGSLVRPQ 603 VVYQVYARQ+SERY+TFTV+PGKSL+AQW GVGPVGGY+LKVYGPNGFLRELRGSL R Sbjct: 541 VVYQVYARQESERYQTFTVQPGKSLTAQWRGVGPVGGYDLKVYGPNGFLRELRGSLERGG 600 Query: 604 LAEPVVQARHDGERGELVLTLENPGAQPLRIVIHDQIHGQATRTLTLAAGQQLRYQWSVA 663 EPVVQARHDGERGELVL L NPG Q L +V+ DQ HGQATRT+TLA GQ +W +A Sbjct: 601 GVEPVVQARHDGERGELVLALLNPGRQALHLVLRDQAHGQATRTVTLAPGQSATERWPLA 660 Query: 664 ASHHWYDLLLQVEDVPGFARRLAGHVETGSPSVFIS 699 +SHHWYDLL+Q ED P FARRLAGHVETG PS F+S Sbjct: 661 SSHHWYDLLVQAEDRPAFARRLAGHVETGRPSFFVS 696 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 76.6 bits (187), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 62/224 (27%), Positives = 100/224 (44%), Gaps = 44/224 (19%) Query: 236 ERLEQHQVSWKVY----QGTDEEQQDHGDYPS--DFNVLQYFFNQYQTAAPDSPLWQ-KA 288 ++L + + W Y Q T +E +D P +F FN ++ P++P + K Sbjct: 335 DKLNKKGIDWAWYAGAWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKR 394 Query: 289 CSKFTLADLAR------DVQGGSLPQVSWLMPP-KLSCEHPDRTPGYGAHYISQVLDILT 341 L D AR DV+ G LP V++ P L+ G +I+ ++ L Sbjct: 395 LRDGGLGDEARTNRFLADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKSLQ 454 Query: 342 ANPALWSSTVLLVNYDENDGFFDHVVPPTPPLHAQQGLSTVDVSGEFHRVGDYVNPADDL 401 A+P W + V++V +DEN G++DHV PP GD P Sbjct: 455 ASPQ-WKNMVVVVTFDENGGWWDHVAPPK---------------------GDRWGP---- 488 Query: 402 PAGLGARVPMLVISPWSKGGYVCSEVFDHTSVIRFLEQVFDVHE 445 G RVP LV+SP+++ G V V+D S++R + + FD+ + Sbjct: 489 ----GTRVPALVVSPFARKGTVDHTVYDTASILRLITRTFDLEK 528 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,553,673 Number of extensions: 121018 Number of successful extensions: 293 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 291 Number of HSP's successfully gapped: 2 Length of query: 699 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 607 Effective length of database: 1,184,250 Effective search space: 718839750 Effective search space used: 718839750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)