BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2595.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 459 e-131 gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 221 5e-59 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 65 4e-12 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 61 1e-10 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 459 bits (1181), Expect = e-131, Method: Compositional matrix adjust. Identities = 222/235 (94%), Positives = 231/235 (98%) Query: 1 MSHALSAFPINQKWPAQHPERIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFESNDQMS 60 M HALS+F I+QKWPAQHP+RIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFE+NDQMS Sbjct: 1 MPHALSSFAIDQKWPAQHPDRIQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMS 60 Query: 61 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKSGQFLPADAAARYQVIQWV 120 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEK+GQFLPADAAARYQVIQWV Sbjct: 61 PEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWV 120 Query: 121 MFQMGGVGPMFGQLGFFHRFAGKDYEDKRPRDRYVNESKRLLGVMEQRLAQSQWLGGDAY 180 MFQMGGVGPMFGQLGFFHRFAGK+YEDKRPRDRYVNESKRLLGVM++RLAQSQWLGGDAY Sbjct: 121 MFQMGGVGPMFGQLGFFHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAY 180 Query: 181 SIADIATFPWIRNLIGFYEAAELVGFHHFPHVARALEAFVARPAVVRGLNIPARG 235 SIADIATFPWIRNLIGFYEAA+LVGF FPHVARALEAFVARPAVVRGLNIPARG Sbjct: 181 SIADIATFPWIRNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGLNIPARG 235 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 221 bits (562), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 109/212 (51%), Positives = 144/212 (67%), Gaps = 7/212 (3%) Query: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFESNDQMSPEFLSLNPNNKIPAIIDPNGP 81 I +YS TPNG KV IMLEE GLPY AH + + DQ +PEFL ++PNNKIPAI+DP+GP Sbjct: 2 IDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGP 61 Query: 82 GGQPLPLFESGAILLYLAEKSGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 G+P+ LFESGAIL+YLA K+G+FL +Y+V+QWVMFQMGG+GPM GQ F +A Sbjct: 62 DGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLYA 121 Query: 142 GKDYEDKRPRDRYVNESKRLLGVMEQRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 + E +RY NE++RL VM+++L Q +L G+ Y+IADIA FPW R+ Sbjct: 122 PEQIE--YAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRS-----HKN 174 Query: 202 ELVGFHHFPHVARALEAFVARPAVVRGLNIPA 233 + + FP+V R +A ARPAV RG+ + A Sbjct: 175 QGIDLDDFPNVKRWYDAISARPAVQRGVTVLA 206 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 65.5 bits (158), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 48/153 (31%), Positives = 73/153 (47%), Gaps = 17/153 (11%) Query: 43 GLPYEAHLVSFESNDQMSPEFLSLNPNNKIPAIIDPNGPGGQPLPLFESGAILLYLAEKS 102 GLP E V + P FL+LNP ++P + D + + +S AIL+YLA+K+ Sbjct: 23 GLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIADSNAILVYLAKKA 76 Query: 103 GQ--FLPADAAARYQVIQWVMFQMG--GVGPMFGQLGFFHRFAGKDYEDKRPRDRYVNES 158 G+ +LP DA V +W+ G GP +L A E + + Sbjct: 77 GRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAFNAEE-------VIQRA 129 Query: 159 KRLLGVMEQRLAQSQWLGGDAYSIADIATFPWI 191 LLG +EQ LA+ WL D +IAD+A + ++ Sbjct: 130 HLLLGRLEQHLAKRDWLAADHPTIADVAVYSYL 162 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 66/225 (29%), Positives = 90/225 (40%), Gaps = 28/225 (12%) Query: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFESNDQMSP-EFLSLNPNNKIPAIIDPNG 80 I ++ L +V +LEE GL YE + ++P E L ++P K P I D Sbjct: 2 ITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE- 60 Query: 81 PGGQPLPLFESGAILLYLAEKSGQ---FLPADAA-ARYQVIQWVMFQMGGVGPMFGQLGF 136 L + ESG I+ YL E+ G LPA R + W+ F G P Sbjct: 61 -----LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQLI 115 Query: 137 FHRFAGKDYEDKRPRDRYVNE----------SKRLLGVMEQRLAQSQWLGGDAYSIADI- 185 F + RP R + E +R L ME L Q W G +S ADI Sbjct: 116 FAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQERQLRFMEDELGQQGWFAGAQFSGADIQ 175 Query: 186 ATFPWIRNLIGFYEAAELVGFHHFPHVARALEAFVARPAVVRGLN 230 +FP + L G +P++ + LEA ARPA R L Sbjct: 176 MSFP----VEALRARGGLDG--RYPNLLKYLEAIHARPAYQRALE 214 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 844,578 Number of extensions: 37518 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 4 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)