BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2587.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 707 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 287 8e-79 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 221 8e-59 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 149 4e-37 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 129 3e-31 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 707 bits (1826), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/386 (90%), Positives = 367/386 (95%) Query: 1 MDFELNEDQLAFQQSARAFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPENEG 60 MDFELN+DQLAFQQSAR FAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPE +G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 61 GLGLSRLDATIVFEELARACTSTAAYLTIHNMVSWMVATWGQGELKEEWCAQLAAGHKIG 120 GLGLSRLDAT+VFEELARACTSTAAYLTIHNMVSWM+A+WGQ LK +WC QLAAG KIG Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGRKIG 120 Query: 121 SYCLTEPGSGSDAASLKTTARRVDDFYLINGSKAFISGAGSTDLLVVMARTGGPGARGVS 180 SYCLTEPGSGSDAASLKTTAR D Y++NGSKAFISGAGSTD+LVVMARTGG GARGVS Sbjct: 121 SYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGEGARGVS 180 Query: 181 AIVVPGDTPGISYGKKESKMGWNSQPTRTISFDNVKVPVGNLLGEEGEGFVFAMKGLDGG 240 AIVVPG+ PG+SYGKKESKMGWNSQPTRTISFDNVKVP +LLG+EG+GF+FAMKGLDGG Sbjct: 181 AIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGG 240 Query: 241 RINIATCSVGAAQAALDAAHAYIKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA 300 RINIATCSVGAAQAALDAAHAY+KERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA Sbjct: 241 RINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLA 300 Query: 301 ATKLDAGSPEATTYCAMAKRLATDLGFNICNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 ATKLDAGSPEATTYCAMAKRLATDLGF +CNEALQLHGGYGYIREYPLERYFRDVRVHQI Sbjct: 301 ATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQI 360 Query: 361 LEGTNEIMRVIISRNLLGRDTLDDIR 386 LEGTNEIMRVIISR LL RD LDDIR Sbjct: 361 LEGTNEIMRVIISRKLLDRDNLDDIR 386 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 287 bits (735), Expect = 8e-79, Method: Compositional matrix adjust. Identities = 151/374 (40%), Positives = 223/374 (59%), Gaps = 3/374 (0%) Query: 5 LNEDQLAFQQSARAFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPENEGGLGL 64 L+ + + + R FA + PFAA WD FP + + + ELG G+ PE GG G+ Sbjct: 3 LSPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGM 62 Query: 65 SRLDATIVFEELARACTSTAAYLTIHNMVSW-MVATWGQGELKEEWCAQLAAGHKIGSYC 123 + + EE+A +T+ +++ N ++ + +G KE+W LA G +G +C Sbjct: 63 DYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFC 122 Query: 124 LTEPGSGSDAASLKTTARRVDDFYLINGSKAFISGAGSTDLLVVMARTG-GPGARGVSAI 182 LTEP +GSDAA+++T A R D +++NG+K FI+ + +V A T G +G+S Sbjct: 123 LTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCF 182 Query: 183 VVPGDTPGISYGKKESKMGWNSQPTRTISFDNVKVPVGNLLGEEGEGFVFAMKGLDGGRI 242 +VP DTPG G+ E KMG ++ T I DN +VP LLG+EGEG+ A+ L+ GRI Sbjct: 183 LVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRI 242 Query: 243 NIATCSVGAAQAALDAAHAYIKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAAT 302 IA SVG A+AA +AA +Y ++R+ FG PI + QA+ F+LADM T L AAR MV AA Sbjct: 243 GIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDAARLMVWRAAQ 302 Query: 303 KLDAGSPEATTYCAMAKRLATDLGFNICNEALQLHGGYGYIREYPLERYFRDVRVHQILE 362 D G P +MAK A++ I ++A+Q+HGG GY ++P+ER +RDVR+ QI E Sbjct: 303 LKDQGRP-CLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 Query: 363 GTNEIMRVIISRNL 376 G N+I R++I R + Sbjct: 362 GANDIQRLVIGRAI 375 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 221 bits (563), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 140/390 (35%), Positives = 209/390 (53%), Gaps = 10/390 (2%) Query: 1 MDFELNEDQLAFQQSARAFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPENEG 60 + F+ ED A +++ AFA E+AP AA+ D FP+D+ K G+LG G+ E G Sbjct: 7 LSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYG 66 Query: 61 GLGLSRLDATIVFEELARACTSTAAYLTIH-NMVSWMVATWGQGELKEEWCAQLAAGHKI 119 G GL L I EE++RA S H N+ + G E K ++ +L +G I Sbjct: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFI 126 Query: 120 GSYCLTEPGSGSDAASLKTTARRVDDFYLINGSKAFISGAGSTDLLVVMARTG-GPGARG 178 G+ ++EP +GSD S+K A + D Y++NGSK +I+ D+LVV A+T GARG Sbjct: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARG 186 Query: 179 VSAIVVPGDTPGISYGKKESKMGWNSQPTRTISFDNVKVPVGNLLGEEGEGFVFAMKGLD 238 ++A +V G S +K K+G T + F + +VP N+LG G G M GLD Sbjct: 187 MTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 Query: 239 GGRINIATCSVGAAQAALDAAHAYIKERKQFGRPIADFQALQFKLADMQTELVAARQMVR 298 R ++ +G QA +D Y+ +RKQFG+ I +FQ +Q KLADM + ++A + V Sbjct: 247 FERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVY 306 Query: 299 LAATKLDAGSPEATTYCAMAKRLATDLGFN------ICNEALQLHGGYGYIREYPLERYF 352 D + A A+ K A + ++ + EA+Q GG GYI EYP+ R + Sbjct: 307 AVGQACDR-ADSADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNGYINEYPVGRLW 365 Query: 353 RDVRVHQILEGTNEIMRVIISRNLLGRDTL 382 RD ++++I GT+EI R++I R L DTL Sbjct: 366 RDAKLYEIGAGTSEIRRMLIGRELFA-DTL 394 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 149 bits (376), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 112/357 (31%), Positives = 183/357 (51%), Gaps = 12/357 (3%) Query: 30 KWDEEAHFPLDVIAKAGELGFCGLYTPENEGGLGLSRLDATIVFEELARAC-TSTAAYLT 88 K DE +P + + G+ P+ GG GL +A+++ EE+ R S A + Sbjct: 31 KIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEASVIMEEINRTGGNSGACHGQ 90 Query: 89 IHNMVSWMVATWGQGELKEEWCAQLAAGH-KIGSYCLTEPGSGSDAASLKTTARRVDDFY 147 ++NM + + G E KE + ++A+G ++ S +TEP +G+D +KT A + D Y Sbjct: 91 MYNMGTLL--RHGSPEQKERYLPRIASGELRLQSMAVTEPTTGTDTTRIKTVAVKKGDRY 148 Query: 148 LINGSKAFISGAGSTDLLVVMARTG-----GPGARGVSAIVVPGDTPGISYGKK-ESKMG 201 +ING K +IS +DL++++ART + G+S +V I +G + + Sbjct: 149 VINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIFLVDLKE-AIGHGMEVRPILN 207 Query: 202 WNSQPTRTISFDNVKVPVGNLLGEEGEGFVFAMKGLDGGRINIATCSVGAAQAALDAAHA 261 + T + FDN+++P NL+GEEG GF + + GL+ R IA +G +D A Sbjct: 208 MVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTLIAAECIGDGYWFIDKATQ 267 Query: 262 YIKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRL 321 Y +ER F RPI Q +QF LA+ E+ AA ++R A L MAK L Sbjct: 268 YARERVVFDRPIGMNQGVQFPLAEAYIEVEAA-NLMRYQACALFDAQQSCGAQANMAKYL 326 Query: 322 ATDLGFNICNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNEIMRVIISRNLLG 378 A + N LQ HGG+G+ EY +ER FR+ R++Q+ + ++ ++ ++LG Sbjct: 327 AAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVAPISTNLIFSYVAEHVLG 383 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 112/372 (30%), Positives = 171/372 (45%), Gaps = 39/372 (10%) Query: 49 GFCGLYTPENEGGLGLSRLDATIVFEELARACTSTAAYLTIHNMVSWMVATWGQGELKEE 108 G+ L GG GL + ++E L+ A Y + + +A G L+ Sbjct: 91 GWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLHGAYACLAAHGSAALQAR 150 Query: 109 WCAQLAAGHKIGSYCLTEPGSGSDAASLKTTARRVDD-FYLINGSKAFISGAGSTDLL-- 165 + ++A+G + + CLTE +GSD L+T A DD Y ++G K FISG G DL Sbjct: 151 YLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISG-GEHDLTPN 209 Query: 166 ---VVMART--GGPGARGVSAIVVP-----GDTPGISYGKKESKMGWNSQPTRTISFDNV 215 +V+AR G++G+S + P G G+ E KMG + T ++ F+ Sbjct: 210 IVHLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQA 269 Query: 216 KVPVGNLLGEEGEGFVFAMKGLDGGRINIATCSVGAAQAALDAAHAYIKERKQFGRP--- 272 + L+GE G G ++ R+++ +G A+AA A AY +ER+Q P Sbjct: 270 QA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSS 326 Query: 273 ----IADFQALQFKLADMQTELVAARQMVRLAATKLDAGS--PEATTYCAMAKRLA---- 322 I A+Q L + + + AR + A LD PEA +RL Sbjct: 327 GNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITP 386 Query: 323 ------TDLGFNICNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNEIMRV-IISRN 375 TD GF + ALQ+ GG+GY+ E +E++ RD R+ I EGTNEI + + R Sbjct: 387 VIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRK 446 Query: 376 LLGRD--TLDDI 385 +LG + LDD Sbjct: 447 VLGDEGRRLDDF 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,169,294 Number of extensions: 49154 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 13 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)