BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2581.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 760 0.0 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 367 e-102 gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 140 2e-34 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 93 5e-20 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 760 bits (1962), Expect = 0.0, Method: Compositional matrix adjust. Identities = 366/403 (90%), Positives = 387/403 (96%) Query: 1 MTTTQRQPGDRRLPVTVLSGFLGAGKTTLLNHILNNREGRRVAVIVNDMSEVNIDAALVR 60 MT++QRQ DRRLPVTVLSGFLGAGKTTLLNHIL+NREGRRVAVIVNDMSEVNIDAALVR Sbjct: 1 MTSSQRQAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVR 60 Query: 61 DGGADLSRTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE 120 DGGADL+RTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE Sbjct: 61 DGGADLARTEEKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAE 120 Query: 121 TFTFTDEEGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLQERGESMGEEDGRTVVDLLI 180 TFTFTDE+GASLSDVARLDTMVTVVDAFNFLKDYSSQDSL +RG+SMGEED RTVVDLLI Sbjct: 121 TFTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLI 180 Query: 181 EQIEFCDVIVLNKLDLVAPADLERLHAILHKLNPRARMVTAQFGKVALDQVLNTGLFDFE 240 EQIEFCDVIVLNKLDLVA DLERLHAILHKLNPRAR+V A+FGKVAL+QVL+TGLFDFE Sbjct: 181 EQIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFDFE 240 Query: 241 QASQAPGWLQELRGEHVPETEQYGITSFAWRARRPMHPQRFFDLVNREWPGVVRSKGFFW 300 QASQAP WLQELRGEHVPETEQYGI+SFAW+ARRP+HPQRFFDL+N EWPGVVRSKGFFW Sbjct: 241 QASQAPSWLQELRGEHVPETEQYGISSFAWQARRPLHPQRFFDLINTEWPGVVRSKGFFW 300 Query: 301 LATRPAHAGSWSQAGAVCRHGLAGKWWAAVPREQWPQEQESIDFILQQWDENVGDARQEL 360 LA+RP AGSWSQAGAVCRHGLAGKWWAAVPREQWPQ+QES+DFIL WDENVGDARQEL Sbjct: 301 LASRPTQAGSWSQAGAVCRHGLAGKWWAAVPREQWPQDQESVDFILAHWDENVGDARQEL 360 Query: 361 VLIGIEMDQQALRARLDACLLSDAEMAQGPLGWSRMADPFPAW 403 VLIG+ MDQ+ALRARLDACLLSDAEMAQGPLGW+RM DPFP W Sbjct: 361 VLIGMAMDQEALRARLDACLLSDAEMAQGPLGWARMRDPFPLW 403 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 193/390 (49%), Positives = 256/390 (65%), Gaps = 10/390 (2%) Query: 11 RRLPVTVLSGFLGAGKTTLLNHILNNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTE 70 +R+PVTVLSGFLG+GKTTLLNHIL NR G +VAVIVNDMSEVN+D VR +L R Sbjct: 15 QRIPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRN-TELYRGN 73 Query: 71 EKLVEMSNGCICCTLREDLLMEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEEGA 130 ++LVEMSNGCICCTLR DLL +V LAR RFD L+IESTGISEP+PVAETF F D +G Sbjct: 74 DELVEMSNGCICCTLRADLLEQVSALARSGRFDYLLIESTGISEPIPVAETFAFLDSDGF 133 Query: 131 SLSDVARLDTMVTVVDAFNFLKDYSSQDSL-QERGESMGEEDGRTVVDLLIEQIEFCDVI 189 SLS++ARLDT+VTVV+ F + + ++ Q G S R + DLLIEQ+E+ +VI Sbjct: 134 SLSELARLDTLVTVVNGETFEQQLADHVAIAQADGAST-----RPLSDLLIEQVEYANVI 188 Query: 190 VLNKLDLVAPADLERLHAILHKLNPRARMVTAQFGKVALDQVLNTGLFDFEQASQAPGWL 249 +++++DL+ + L A+L KLNP AR++ G+V LDQVL+T LFD ++ PGW+ Sbjct: 189 LVSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLFDLPSLARMPGWM 248 Query: 250 QELRGEHVP-ETEQYGITSFAWRARRPMHPQRFFDLVNREWPG--VVRSKGFFWLATRPA 306 Q + G H P E + YGI+S +RAR P HP R D + R W ++R KG+ W+++R + Sbjct: 249 QTMDGAHPPSEADTYGISSSVYRARLPFHPGRLLDWLGRPWRNGRLLRCKGYLWISSRYS 308 Query: 307 HAGSWSQAGAVCRHGLAGKWWAAVPREQWPQEQESIDFILQQWDENVGDARQELVLIGIE 366 Q G + G G+WW + QWPQ+ I ++WD GD RQELV IG Sbjct: 309 DIALLVQTGGQFQWGFVGRWWRFIEPAQWPQDTYRRQGIQEKWDALAGDCRQELVFIGQG 368 Query: 367 MDQQALRARLDACLLSDAEMAQGPLGWSRM 396 +D L LDACLL+ E+ GP W+++ Sbjct: 369 IDGDLLTEELDACLLTTTEIEAGPDAWAQL 398 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 140 bits (354), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 90/250 (36%), Positives = 136/250 (54%), Gaps = 36/250 (14%) Query: 9 GDRRLPVTVLSGFLGAGKTTLLNHILNNREGRRVAVIVNDMSEVNIDAALVRDGGADLSR 68 GD +PV++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV Sbjct: 8 GDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHS------ 61 Query: 69 TEEKLVEMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAETF 122 EE +VEMS+GC+CCT+R DL ++ + R+F+++VIE+TG++ P P+ T Sbjct: 62 QEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHTL 121 Query: 123 TFTDEEGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLQERGESMGEEDGRTVVDLLIEQ 182 TD A +++ RLD ++ VDA N + S+ D +E ++Q Sbjct: 122 -MTD---AFIAERYRLDGVIVTVDAVNGM---STLDHHEEA----------------VQQ 158 Query: 183 IEFCDVIVLNKLDLVAPADLERLHAILHKLNPRARMVTAQFGKVALDQVLNTGLFD-FEQ 241 D ++L K DLV A L L A L LNP AR + Q G++A ++L+ GLF E+ Sbjct: 159 AAVADRLLLTKTDLVDAATLAALQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEK 218 Query: 242 ASQAPGWLQE 251 WL E Sbjct: 219 MPDVARWLNE 228 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 92.8 bits (229), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 72/226 (31%), Positives = 118/226 (52%), Gaps = 21/226 (9%) Query: 13 LPVTVLSGFLGAGKTTLLNHILNNREGRRVAVIVNDMSEVNIDAALVRDGGADLSRTEEK 72 +PVTV++GFLGAGKTTLL +L R+ RR+A+++N+ EV ID L+R G ++ + + Sbjct: 17 IPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRAEGD--AQGQVQ 74 Query: 73 LVEMSNGCICCTLREDLL-MEVRKLARERRFDQLVIESTGISEPLPVAETFTFTDEEGAS 131 + + ++G I + + AR + D ++IE++G++ P V E +GA Sbjct: 75 IQDFAHGLIAYGDDAHFVPAMLALAARRAQIDHVLIETSGLALPSAVMEVL-----QGAE 129 Query: 132 LSDVARLDTMVTVVDAFNFLKD-YSSQDSLQERGESMGEEDGRTVVDLLIEQIEFCDVIV 190 ++ LD + VVD L+D ++S D++ G L +Q+E DV+V Sbjct: 130 MARHFVLDATLAVVDTPLLLEDAFASADAV-----------GAATAQLFRQQLECADVVV 178 Query: 191 LNKLDLVAPADLERLHAILHKLNPRARMVTAQFGKVALDQVLNTGL 236 LNK+D + A L + + L P R + +G LD L GL Sbjct: 179 LNKIDGMEEAPLLQAEQAVRALAPNVRFLELAYG-ARLDIRLALGL 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,319,847 Number of extensions: 57299 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 231 Number of HSP's successfully gapped: 4 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)