BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0001.2577.p
         (334 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic d...   536   e-153

>gi|300311855|ref|YP_003775947.1| sphingosine kinase/eukaryotic
           diacylglycerol kinase [Herbaspirillum seropedicae SmR1]
          Length = 339

 Score =  536 bits (1380), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 270/338 (79%), Positives = 291/338 (86%), Gaps = 5/338 (1%)

Query: 1   MPDQHPTTDFPANN-GRPDVVAVINAKAGGGHAEKLANHITDQFASHGLRARVHLAHSGQ 59
           MPDQ+ T+  P ++  RPDVVAVINAKAGGGHAE+LA  I+ +FA HG  ARV LA SG+
Sbjct: 1   MPDQNTTSPLPPHSQARPDVVAVINAKAGGGHAEELAARISAEFARHGRHARVRLAASGE 60

Query: 60  DMLQVARQAVREKVPVVAAGGGDGSINAVASILLAEPQCQSALGVLPLGTLNHFAKDLNI 119
           DML  ARQAVR++VPVVA GGGDGS+NAVAS LLA+ QCQSALGVLPLGTLNHFAKDLNI
Sbjct: 61  DMLATARQAVRDRVPVVAVGGGDGSVNAVASTLLADAQCQSALGVLPLGTLNHFAKDLNI 120

Query: 120 PLALEEAIANIASGRRIKVDSGEVNGAAFINNSSLGLYPDIVREREKQQARLGRGKWLAF 179
           PL LE AIANIA+GRR++VDS EVNG AFINNSSLGLYPDIVREREKQQARLGRGKWLAF
Sbjct: 121 PLTLEGAIANIATGRRLRVDSAEVNGVAFINNSSLGLYPDIVREREKQQARLGRGKWLAF 180

Query: 180 TWAAMGALRRYPFLHVRLSIDGQEHWRRTPFVFIGNNAYLMRGLDIGKRSTLTEGRLSLY 239
           TWAAMGALRRYPFL VRLSIDGQEHWRRTPFVFIGNN YLM GLDIGKRS+LTEG LSLY
Sbjct: 181 TWAAMGALRRYPFLRVRLSIDGQEHWRRTPFVFIGNNPYLMSGLDIGKRSSLTEGCLSLY 240

Query: 240 ACHHTGRWGLLRLALHALFGRLHEAHDFDVLTATEILIETHRKRMRVATDGEVNLMATPL 299
            CHHTGRWGLLRLALHALFGRL +AHDFD+LTAT+I IETH++RMRVATDGEV LM TPL
Sbjct: 241 VCHHTGRWGLLRLALHALFGRLRQAHDFDMLTATDIHIETHKRRMRVATDGEVQLMQTPL 300

Query: 300 HYRIRPASLEVIVPQ----VAAQPRNGQAGLLDKLLGE 333
            YRIRPASLEVIVPQ     A        GLLDKL GE
Sbjct: 301 SYRIRPASLEVIVPQAPASAATTASPPTGGLLDKLWGE 338


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.137    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,155,892
Number of extensions: 50772
Number of successful extensions: 170
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 170
Number of HSP's successfully gapped: 1
Length of query: 334
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 248
Effective length of database: 1,212,660
Effective search space: 300739680
Effective search space used: 300739680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)