BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2571.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309753|ref|YP_003773845.1| HipA domain-containing protein ... 357 e-100 gi|300313385|ref|YP_003777477.1| HipA domain-containing protein ... 82 1e-16 gi|300312001|ref|YP_003776093.1| HipA-like protein [Herbaspirill... 63 5e-11 >gi|300309753|ref|YP_003773845.1| HipA domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 455 Score = 357 bits (917), Expect = e-100, Method: Compositional matrix adjust. Identities = 213/442 (48%), Positives = 265/442 (59%), Gaps = 14/442 (3%) Query: 2 QQLHAWMNGELVGTWSVERQLHRFHYAPQWVRSVHFRPLSLSLPL-TASGELRGDAVRHY 60 ++L WMNG VGTW++ Q Y +WV S RPLSLSLP + RGD VRHY Sbjct: 10 KELGLWMNGSFVGTWALTAQGDSLQYDARWVVSASGRPLSLSLPFRPGNPPHRGDLVRHY 69 Query: 61 FDNLLPDNADIRERLRQRYRLASIEVFDLLQALGRDCVGALQLLPPGQEPQGWRSIQVER 120 F+NLLPD+ IRERL +RY+ A + F LL GRDCVGALQ+LP G P+ +I Sbjct: 70 FENLLPDSKAIRERLARRYQAADTDAFALLGEAGRDCVGALQILPLGATPELSWTIDARP 129 Query: 121 LDESAVEQLLLALPLSSPPASQQEEALRISIAGAQEKTALTWHRGKWCRPLGATPTTHII 180 L E V +LL +P A Q E LR+SIAGAQEKTAL + +WC P GATPTTHI+ Sbjct: 130 LREREVAELLRQTMEPTPLALTQGEELRLSIAGAQEKTALLRYLDQWCLPHGATPTTHIL 189 Query: 181 KLPLGLIGGVMQRVDAGDSVYNEWLCSRILAALGLPVAPARVEHFGRHTVLAVERFDRVK 240 KLP+GL+GG+ ++D SV NEWLCSRILAA G+PVA + F VLAVERFDR Sbjct: 190 KLPMGLVGGL--QLDLRHSVENEWLCSRILAAYGMPVAACDIFCFEDQKVLAVERFDRRW 247 Query: 241 MKE-----GWIARLPQEDFCQAFGLPPTLKYESQGGPGLAHCLGLL-NASSQGGDKTYFL 294 ++ + RLPQED CQA G+ P LKYE+ GGPG+ + LL A + +F Sbjct: 248 SRDPASGQTMLLRLPQEDMCQATGISPWLKYEADGGPGMKEIMALLGGAIDPVAARRHFF 307 Query: 295 LAQLAFFLLAATDGHAKNFSLHLHAGGRYEMTPLYDVLSMWPYFGKGPNQFRARQAGPAM 354 AQL F++L ATDGHAKNFSL L AGGRYE TPLYDVLS++P G+GP + +A AM Sbjct: 308 KAQLLFWMLWATDGHAKNFSLFLKAGGRYEPTPLYDVLSVFPLIGEGPGKLSPHRAAMAM 367 Query: 355 ILRGRNAHRYFHTIEARHWRQLALQHG-----GEPVWQAMLALVQGSDAALEVVQAQLPA 409 +R +NAHR I RHW L G G+ + L + ++ + VQA LP Sbjct: 368 AVRAKNAHRKMKDILPRHWMALGSHFGIVAENGDGPLAILEELAEQTEQVIAKVQADLPP 427 Query: 410 DFPLRTWERISQGVRSHRERFL 431 FP I G+R L Sbjct: 428 GFPSELAASILDGLRRSASSLL 449 >gi|300313385|ref|YP_003777477.1| HipA domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 81.6 bits (200), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 94/341 (27%), Positives = 136/341 (39%), Gaps = 46/341 (13%) Query: 24 RFHYAPQWVRSVHFRPLSLSLPLTASGELRGDAVRHYFDNLLPDNADIRERLRQRYRLAS 83 F+Y W S PLS L+ G + A ++ NL P+ D R + + RL + Sbjct: 29 NFYYYAHWQLSTETFPLSPHFALS-QGRFKSSATANFMMNLFPE-GDARMAIMKHSRLNA 86 Query: 84 IEVFDLLQALGRDCVGALQLL--------PPGQEPQGWRSIQVERLDESAVEQLLLALPL 135 VF + G + GA L P Q P R I + L E + Sbjct: 87 DNVFSMAYLFGAEPAGAASFLFHSGTSALPSSQAPY-LREIDDDELSER------IHARH 139 Query: 136 SSPPASQQEEALRISIAGAQEKTALTWHRGKWCRPLGATPTTHIIK-LPLGLIGGVMQRV 194 P +Q A S+AG Q+K + +H + G+ +THI+K P G +R Sbjct: 140 RGVPFAQWHGAWHQSLAGVQDKLQVIFHDERLFLAGGSLTSTHILKPEPQG------ERT 193 Query: 195 DAGDSVYNEWLCSRILAALGLPVAPARVEHFGRHTVLAVERFDRVK------MKEGWIAR 248 V NE C + AA+GL V ++ H VL ++RFDR ++ G + R Sbjct: 194 P--HLVANEHFCMSLAAAVGLSVPHVKI-HRAPEAVLLIDRFDRTYAPGTDVLQPGRVER 250 Query: 249 LPQEDFCQAFGLPPTLKYESQGG-----PGLAHCLGL-----LNASSQGGDKTYFLLAQL 298 D CQA LP KYE GG P + + L + L+ + Sbjct: 251 THIIDGCQALELPSWYKYECLGGHQNRSPERRYGIDFKRLFGLAPHFEDASAAKLLIVRW 310 Query: 299 AFF--LLAATDGHAKNFSLHLHAGGRYEMTPLYDVLSMWPY 337 A F L+ +D H KN S A G P YD++S+ Y Sbjct: 311 ALFNLLIGNSDAHGKNISFRQTANGIMP-APFYDLVSVQVY 350 >gi|300312001|ref|YP_003776093.1| HipA-like protein [Herbaspirillum seropedicae SmR1] Length = 446 Score = 62.8 bits (151), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 92/301 (30%), Positives = 126/301 (41%), Gaps = 53/301 (17%) Query: 60 YFDNLLPDNADIRERLRQRYRLASIEVFDLLQALGRDCVGALQLLPPGQEPQGWRSIQVE 119 +F NLLP+ +R+ L QR LA + F LL GRD G + S E Sbjct: 80 FFQNLLPEG-QLRKHLVQRAGLAPDDEFGLLAYCGRDLPGDV-------------SASAE 125 Query: 120 RLDESAVEQLLLALPLSSPPASQQEEALRI----SIAGAQEKTALTWHRG-KWCRPLGAT 174 LDE + +LL S +S Q L + S++G Q K AL G ++ + Sbjct: 126 ELDERGLGRLLGQGHDSYEMSSGQ---LPVPGGESLSGVQPKLALVREAGGRYVMRSKDS 182 Query: 175 PTTHII-KLPLGLIGGVMQRVDAGDSVYNEWLCSRILAALGL--------PVAPARVEH- 224 +H I KLP D + E+ + AA G+ P++ A EH Sbjct: 183 HGSHFIAKLP---------ATDYPNMPQVEFSSLTLAAAAGVHTCRFSLEPLS-AIAEHL 232 Query: 225 -FGRHT----VLAVERFDRVKMKEGWIARLPQEDFCQAFGLPPTLKYESQGGPGLAHCLG 279 FG L V+RFDR RL EDF Q G P KY G A + Sbjct: 233 PFGLRNDASRFLLVQRFDRDAPTP--TGRLHMEDFAQVTGTAPGAKYV---GSYAAIGVV 287 Query: 280 LLNASSQGGDKTYFLLAQLAFF-LLAATDGHAKNFSLHLHAGGRYEMTPLYDVLSMWPYF 338 LL S++G + Y LL ++ LL D H KNFSL H ++P YD+++ Y Sbjct: 288 LLERSTRGVEDVYELLRRIKVNELLGNFDAHLKNFSLLYHTPQAAALSPAYDIVAYAAYV 347 Query: 339 G 339 G Sbjct: 348 G 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,509,383 Number of extensions: 65732 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 3 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)