BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2544.p (118 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311825|ref|YP_003775917.1| hypothetical protein Hsero_2510... 206 4e-55 >gi|300311825|ref|YP_003775917.1| hypothetical protein Hsero_2510 [Herbaspirillum seropedicae SmR1] Length = 118 Score = 206 bits (524), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 98/114 (85%), Positives = 107/114 (93%) Query: 1 MSEKLSAERHTPDELSQELEALDLEIVRFAVICQVRLFDPGVLNHVMDNNEQVCGQPHPT 60 M+EKLSA+RHTPDELSQELEALD+EIVR AVICQVRLFDPG+LN VMDNNEQVCG+PHPT Sbjct: 1 MTEKLSAQRHTPDELSQELEALDMEIVRHAVICQVRLFDPGILNQVMDNNEQVCGEPHPT 60 Query: 61 AFQSLRGLLYLHFDMHRQLAQTYGAADAEQIIQRVHEHLRPRIGEQLGSLFDTR 114 AFQSLRGLLYLHFDMHRQLA+TYG ADAE II+RVH HL+PRIGEQL SLF+ R Sbjct: 61 AFQSLRGLLYLHFDMHRQLAETYGPADAEVIIERVHAHLKPRIGEQLHSLFERR 114 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 362,399 Number of extensions: 13031 Number of successful extensions: 31 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 31 Number of HSP's successfully gapped: 1 Length of query: 118 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 44 Effective length of database: 1,269,480 Effective search space: 55857120 Effective search space used: 55857120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 124 (52.4 bits)