BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2539.p (796 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 1435 0.0 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 993 0.0 gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 129 8e-31 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 62 2e-10 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 1435 bits (3715), Expect = 0.0, Method: Compositional matrix adjust. Identities = 715/803 (89%), Positives = 742/803 (92%), Gaps = 8/803 (0%) Query: 1 MSGMSVMAYRAPGEPMETSPEASPASAPPSP--------ESTALSLSFEEQGLLEALLAG 52 MSGMSVMAYRA E E S +AS A + E+TALSLSFEEQG+LEALLAG Sbjct: 1 MSGMSVMAYRASVESPEASSDASFAPPSSASSADSIHPLETTALSLSFEEQGMLEALLAG 60 Query: 53 RLAQPFGLLGPRYASQTGGVSAIRTFHPGAAAVRVRCRQSGVLLGELQPFQIDGQPSGFF 112 RL QPF LLGPRYAS G +A+R FHPGA AVRVRCR SGVLL ELQ + +DGQPSGFF Sbjct: 61 RLTQPFDLLGPRYASAKGDGAAVRCFHPGAEAVRVRCRHSGVLLAELQQYPVDGQPSGFF 120 Query: 113 SGVASGLDQRRHQVPYVLEVDWLIPDGNRSVQTTEDPYAFGLLLGELDLHLLQEGRHRQL 172 SG+ GL+QRRH+VPYVLEVDW IPDG+RSVQ+TEDPYAFGLLLGELDLHLLQEGRHRQL Sbjct: 121 SGLVPGLEQRRHEVPYVLEVDWRIPDGSRSVQSTEDPYAFGLLLGELDLHLLQEGRHRQL 180 Query: 173 ADCLGARPMEIDGIPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPR 232 ADCLGARPMEIDG+PGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPR Sbjct: 181 ADCLGARPMEIDGVPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPR 240 Query: 233 IGPGDVYKYEILGRGGVLLPLKADPVARATEAPPATASVVVDARPYLWQDQQWMAERAQR 292 IGPGDVYKYEI GRGGVLLPLKADPVARATE PPATASVV DARPY+WQDQQWMAERAQR Sbjct: 241 IGPGDVYKYEIRGRGGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQR 300 Query: 293 QSLAAPMSIYELHPASWRRIPEQAHRNLNWDELAEQLIPYVVAMGFTHIELMPVMEHPFG 352 QSL+APMSIYE+HPASWRR+PEQ HRNLNWDELA+QLIPYVV MGFTHIELMPVMEHPFG Sbjct: 301 QSLSAPMSIYEVHPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFG 360 Query: 353 GSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDEHGLAQFDGTA 412 GSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSD+HGLAQFDGTA Sbjct: 361 GSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTA 420 Query: 413 LYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDY 472 LYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDY Sbjct: 421 LYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDY 480 Query: 473 SRKSGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVTAAVKEETD 532 SRK GEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVTA VKEE D Sbjct: 481 SRKEGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMIAEESTSWPGVTAPVKEEGD 540 Query: 533 HHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHG 592 HHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHG Sbjct: 541 HHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLLYGFSENFILPISHDEVVHG 600 Query: 593 KRALLNKMPGDQWRRFANVRAYYGFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDE 652 KRALLNKMPGDQWRRFANVRAY+GFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDE Sbjct: 601 KRALLNKMPGDQWRRFANVRAYFGFMWTHPGKKLLFMGSEFGQPDEFDHDKSPYWHLLDE 660 Query: 653 HGDHPAGHRQLRQLVSDLNHLYRSEPALHQRDCRADGFSWAVGDDSVNSVLAYFRYGEEG 712 H HPA HRQLRQLV DLN LY SEPALHQRDCRADGF+WAVGDDSVNSVLAYFRY +EG Sbjct: 661 HAGHPAEHRQLRQLVGDLNRLYCSEPALHQRDCRADGFAWAVGDDSVNSVLAYFRYADEG 720 Query: 713 PPVLVVVNLTPVPRSAYRIGVPPIGPGHNGVWREVLNTDAAVYGGSNSGNMGQVQVQESG 772 PPVLVVVNLTPVPR YRIGVP I PGH+GVWREVLNTDA +YGGSN+GN+G VQVQE Sbjct: 721 PPVLVVVNLTPVPRVNYRIGVPSIAPGHHGVWREVLNTDAGLYGGSNNGNLGAVQVQELA 780 Query: 773 WHGHPVSIELNLPPLSTLILRRE 795 WHGH SIEL LPPLSTLILRRE Sbjct: 781 WHGHAASIELTLPPLSTLILRRE 803 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 993 bits (2566), Expect = 0.0, Method: Compositional matrix adjust. Identities = 510/821 (62%), Positives = 597/821 (72%), Gaps = 54/821 (6%) Query: 13 GEPMETSPEASPASAPPSPESTALSLSFEEQGLLEALLAGRLAQPFGLLGPRY------A 66 G M S S AP +P S + ++ L+ L+ GRL PF LLGPR A Sbjct: 13 GAAMPASRRKSLTEAPGAPPSEVVL----DESLVARLMQGRLGDPFALLGPREIADAPEA 68 Query: 67 SQTGGVSAIR--TFHPGAAAVRVRCRQS------------GVLLGELQPF---------- 102 +Q +R + PGA+AV+V R S G LLG L + Sbjct: 69 TQKAKAKRLRLCCYFPGASAVQVYARTSAAPDELHAPDSTGSLLGSLHEWSPATGQGESD 128 Query: 103 ---QIDGQPSGFFSGVASGLDQRRHQ--VPYVLEVDWLIPDGNRSVQTTEDPYAFGLLLG 157 + Q SG F G L +R Q +PYV V W DG ++Q TED YAFGLLL Sbjct: 129 HDQEAPRQRSGVFFGEVK-LPRRAVQPPIPYVFHVTWRGADGRETLQITEDAYAFGLLLS 187 Query: 158 ELDLHLLQEGRHRQLADCLGARPMEIDGIPGTRFAVWAPNAERVSVVGDFNQWDGRRHVM 217 + DLHL +EGRHRQLA CLG++ M+IDG+ G RFAVWAPNA+RVSV+GDFNQWDGRR+ M Sbjct: 188 DFDLHLFREGRHRQLARCLGSQVMQIDGVEGVRFAVWAPNAQRVSVIGDFNQWDGRRNPM 247 Query: 218 RLRHEPGVWELFIPRIGPGDVYKYEILGRGGVLLPLKADPVARATEAPPATASVVVDARP 277 RLR GVWELF+PR+G G +YKYEI+G G LLP KADP+ARATE PPATAS V+D Sbjct: 248 RLRQSAGVWELFVPRLGDGALYKYEIIGPHGELLPGKADPMARATEVPPATASRVIDDSK 307 Query: 278 YL----WQDQQWMAERAQRQSLAAPMSIYELHPASWRRIPEQAHRNLNWDELAEQLIPYV 333 + WQD W+ +RAQ Q+ APMSIYE+H ASW R+P + +RNL+W+ELA++LIPYV Sbjct: 308 LVSGRRWQDAHWLQQRAQGQTRQAPMSIYEVHAASWLRLPGEGNRNLDWNELADRLIPYV 367 Query: 334 VAMGFTHIELMPVMEHPFGGSWGYQPISMFAPSARFGKPERFAAFVDRCHQAGIGVLLDW 393 MGFTH+EL+PVMEHPFGGSWGYQP+S FAPSARFG P FA FVD CH+AGIGVLLDW Sbjct: 368 KGMGFTHVELLPVMEHPFGGSWGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDW 427 Query: 394 VPAHFPSDEHGLAQFDGTALYEHADPREGFHQDWNTLIFNLGRNEVRGFLIASALEWLEH 453 VPAHFPSD HGLA FDGT LYEH DPREG HQDWNT I+NLGR+EVRGFLIASALEWLEH Sbjct: 428 VPAHFPSDPHGLAYFDGTPLYEHGDPREGIHQDWNTHIYNLGRHEVRGFLIASALEWLEH 487 Query: 454 FHVDGLRVDAVASMLYRDYSRKSGEWVPNIHGGRENLEAVAFLRELNTVVAQRCPGALMI 513 FHVDGLRVDAVASMLYRDYSR SG+WVPNIHGGRENLEAV FLRELN VA+RCPGALMI Sbjct: 488 FHVDGLRVDAVASMLYRDYSRSSGQWVPNIHGGRENLEAVDFLRELNQTVAERCPGALMI 547 Query: 514 AEESTSWPGVTAAVKEETDHHGLGFSYKWNMGWMNDTLRYMQHDPLFRRYHHHDMTFGLL 573 AEEST+WPGVTA V E GLGF+YKWNMGWM+DTLRY++ D ++RRYHHHD+TFG++ Sbjct: 548 AEESTAWPGVTAPVSEG----GLGFTYKWNMGWMHDTLRYVELDSIYRRYHHHDITFGMI 603 Query: 574 YGFSENFILPISHDEVVHGKRALLNKMPGDQWRRFANVRAYYGFMWTHPGKKLLFMGSEF 633 Y FSE+F+LPISHDEVVHGK LL KM GD W+RFAN+RAY GFMWTHPGKKLLFMG EF Sbjct: 604 YAFSESFVLPISHDEVVHGKGTLLQKMAGDAWQRFANLRAYLGFMWTHPGKKLLFMGCEF 663 Query: 634 GQPDEFDHDKSPYWHLLDEHGDHPAGHRQLRQLVSDLNHLYRSEPALHQRDCRADGFSWA 693 + EFD D+SP+W LLD+ A HR +++LV DLN LY PAL++ D A GFSW Sbjct: 664 AETREFDPDRSPHWDLLDD-----AHHRGVQRLVRDLNRLYAGTPALYRHDTEAKGFSWV 718 Query: 694 VGDDSVNSVLAYFRYGEEGPPVLVVVNLTPVPRSAYRIGVPPIGPGHNGVWREVLNTDAA 753 VG+D +NSV AY R ++ PVLVV+N+TPVPR YRIGVPP G W E+LNTDA Sbjct: 719 VGNDELNSVFAYLRQTDDEAPVLVVINMTPVPRHHYRIGVPP-GQTQQDRWEEILNTDAD 777 Query: 754 VYGGSNSGNMGQVQVQESGWHGHPVSIELNLPPLSTLILRR 794 YGGS+ GN GQ++ Q HGH S+ L LPPLSTL+LRR Sbjct: 778 CYGGSHLGNGGQLRAQAQACHGHASSLVLTLPPLSTLVLRR 818 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 129 bits (325), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 103/310 (33%), Positives = 140/310 (45%), Gaps = 41/310 (13%) Query: 189 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRHEPGVWELFIPRIGPGDVYKY--EILGR 246 RF VWAP A V+V D R + + EP W PG Y+Y + + + Sbjct: 21 VRFRVWAPGARSVAV--DITG----RGRLPMDAEPQGWFALETACDPGATYRYVFDTISQ 74 Query: 247 GGVLLPLKADPVARATEAPPATASVVVDARPYLWQDQQWMAERAQRQSLAAPMSIYELHP 306 G ++ P DP +R S+VVDA+ Y+WQ QW + L YELHP Sbjct: 75 GELVAP---DPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEETVL------YELHP 125 Query: 307 ASWRRIPEQAHRNLNWDELAEQLIPYVVAMGFTHIELMPVMEHPFGGSWGYQPISMFAPS 366 + R ELAE +G T IELMPV E P +WGY + FAP Sbjct: 126 GAMGGFAGIRQR---LAELAE--------LGITAIELMPVAEFPGAHNWGYDGVLPFAPD 174 Query: 367 ARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDEHGLAQFDGTALYEHADPREGFHQD 426 A +G PE+ A +D H G+ V LD V HF D + Y HA E F QD Sbjct: 175 ASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGN----------YLHAYAPEFFRQD 224 Query: 427 WNTL---IFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKSGEWVPNI 483 TL + R EV F +AL WL+ + DGLR+DAV ++ + + G+ + Sbjct: 225 MQTLWGATIDFRRPEVDDFFTQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIRTE 284 Query: 484 HGGRENLEAV 493 GG+ ++ V Sbjct: 285 IGGQRHVHLV 294 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 62.0 bits (149), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 83/345 (24%), Positives = 133/345 (38%), Gaps = 72/345 (20%) Query: 184 DGIPGTRFAVWAPNAERVSVVGDFNQWDGRRHVMRL---RHEPGVWELFIPRIGPGDVYK 240 DG+ G FAV++ +A R+ + G+R + R+ VW ++P PG VY Sbjct: 22 DGL-GVNFAVFSAHATRMDLC--LFDPSGKRELKRIALPECTDEVWHGYLPGAAPGLVYG 78 Query: 241 YEILG-----RGGVLLPLKA--DPVARAT---------------------------EAPP 266 + G +G P K DP A+ ++ Sbjct: 79 FRAHGPYEPNKGHRFNPNKLLLDPYAKRLVGQVRWTDALYGYRLHSPRADLSFDRRDSAA 138 Query: 267 ATASVVVDARPYLWQDQQWMAERAQRQSLAAPMSIYELHPASWRRIPEQA--HRNLNWDE 324 A +V ++W+ + + + +++ IYE H R+ E H + Sbjct: 139 AMPKAMVTEDAFVWEHDR-LPQVPWNKTV-----IYEAHVKGISRLREDLLPHERGTFAA 192 Query: 325 LAE-QLIPYVVAMGFTHIELMPV---------MEHPFGGSWGYQPISMFAPSARF---GK 371 LA+ I +++ +G T +ELMPV +E WGY +S FAP + G Sbjct: 193 LADPAFIDHLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGD 252 Query: 372 PERFAAFVDRCHQAGIGVLLDWVPAHFPSDEH-----GLAQFDGTALYEHADPREGFHQD 426 P V R H AGI V+LD V H D + + +E +H + Sbjct: 253 PNELRMAVRRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHIN 312 Query: 427 ----WNTLIFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASM 467 NTL NL V ++ S W + FH+DG R D +++ Sbjct: 313 DTGCGNTL--NLSHPRVLQMVLDSLRYWAQSFHIDGFRFDLGSTL 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,749,156 Number of extensions: 125142 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 4 Length of query: 796 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 703 Effective length of database: 1,179,515 Effective search space: 829199045 Effective search space used: 829199045 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)