BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2537.p (630 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalo... 1068 0.0 gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching prot... 120 3e-28 gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching prot... 100 5e-22 gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme pro... 92 1e-19 >gi|300311816|ref|YP_003775908.1| malto-oligosyltrehalose trehalohydrolase [Herbaspirillum seropedicae SmR1] Length = 616 Score = 1068 bits (2762), Expect = 0.0, Method: Compositional matrix adjust. Identities = 536/630 (85%), Positives = 566/630 (89%), Gaps = 14/630 (2%) Query: 1 MSARFSVTLPFGAQVSAPGQVRFRVWAPGAEAVAVEIIGRERLPMTPEDAESPAWYVLEV 60 MSARFS LPFGAQV+ PG+VRFRVWAPGA +VAV+I GR RLPM DAE W+ LE Sbjct: 1 MSARFSWNLPFGAQVTEPGRVRFRVWAPGARSVAVDITGRGRLPM---DAEPQGWFALET 57 Query: 61 PCDPGTLYRYVFQTRAQGEVIAPDPASRGQAGDVFDPSVVVDPQAYVWQYPHWQGRPWEE 120 CDPG YRYVF T +QGE++APDPASRGQAGDVFDPS+VVD QAYVWQYP W+GRPWEE Sbjct: 58 ACDPGATYRYVFDTISQGELVAPDPASRGQAGDVFDPSLVVDAQAYVWQYPQWRGRPWEE 117 Query: 121 TVLYELHPGAMGGFAGIQQRLGELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASY 180 TVLYELHPGAMGGFAGI+QRL ELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASY Sbjct: 118 TVLYELHPGAMGGFAGIRQRLAELAELGITAIELMPVAEFPGAHNWGYDGVLPFAPDASY 177 Query: 181 GTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGNFLHCYAPEFFRQDRQTLWGASIDFRR 240 GTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGN+LH YAPEFFRQD QTLWGA+IDFRR Sbjct: 178 GTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGNYLHAYAPEFFRQDMQTLWGATIDFRR 237 Query: 241 PEVADFFTQNALYWLQEYRFDGLRLDAVHAICEPDWLVTLGQRIRAEIGSARHVHLVLEH 300 PEV DFFTQNALYWLQEYRFDGLRLDAVHAICEP WL LGQRIR EIG RHVHLVLEH Sbjct: 238 PEVDDFFTQNALYWLQEYRFDGLRLDAVHAICEPAWLERLGQRIRTEIGGQRHVHLVLEH 297 Query: 301 DGNAAHLLGYTAAAEAEAEVAEIGQVRQRSSQVYDAQWNDDAHHVLHVLLTGEDSGYYSA 360 DGNAAHLLG+ + RSS VYDAQWNDDAHHVLHVLLTGEDSGYY+A Sbjct: 298 DGNAAHLLGHGHPPD-----------DGRSSHVYDAQWNDDAHHVLHVLLTGEDSGYYAA 346 Query: 361 YADAPAEKLARCLQQGFVYQGEVSPYSGEPRGEPSADLPPTAFVNFLQNHDQIGNRAFGE 420 YA+ PA KLARCLQQGFVYQGEVSPYSGEPRGEPSADLPPTAFVNFLQNHDQIGNRAFGE Sbjct: 347 YAEEPAAKLARCLQQGFVYQGEVSPYSGEPRGEPSADLPPTAFVNFLQNHDQIGNRAFGE 406 Query: 421 RLSVLAHPLALHAAQALILLAPQVPMLFMGEEFCAVQPFLYFTSHRDEALVAAVREGRRR 480 RL+VLAHPLALHAAQAL+LLAPQ+PMLFMGEEFCAVQPFLYFTSHRDEALV AVREGRRR Sbjct: 407 RLTVLAHPLALHAAQALVLLAPQIPMLFMGEEFCAVQPFLYFTSHRDEALVKAVREGRRR 466 Query: 481 EFARFPQFADPKLLEQIPDPNSFATFVSSIPEPGPAPASAACLRRVAELLHLRKHHIAPY 540 EFARFPQFADP LLEQIPDPNSFATFVSSIPEPGPAP SAACLRRVAELLH+RK HIAPY Sbjct: 467 EFARFPQFADPALLEQIPDPNSFATFVSSIPEPGPAPGSAACLRRVAELLHIRKLHIAPY 526 Query: 541 LRGAKALEARAIGPKAVHARWQLSNGSVLNITVNLDQAPLQETLDDLAQPAGADVLFDSG 600 LRGA+ALEARAIGPKAVHARW+LSNGSVLNITVNL + +QE+LD LAQ AGADVLFDSG Sbjct: 527 LRGARALEARAIGPKAVHARWRLSNGSVLNITVNLAEQAVQESLDQLAQSAGADVLFDSG 586 Query: 601 GALQALAEGSFPAHAILALREPASQAGRAA 630 GAL+A+AEG FP HAILALREPA+ GR+A Sbjct: 587 GALEAVAEGRFPGHAILALREPATGDGRSA 616 >gi|300311818|ref|YP_003775910.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 804 Score = 120 bits (301), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 99/299 (33%), Positives = 132/299 (44%), Gaps = 42/299 (14%) Query: 21 VRFRVWAPGAEAVAV-----EIIGRERLPMTPEDAESPAWYVLEVPCDPGTLYRYVFQTR 75 RF VWAP AE V+V + GR + E W + PG +Y+Y + R Sbjct: 197 TRFAVWAPNAERVSVVGDFNQWDGRRHVMRLRH--EPGVWELFIPRIGPGDVYKY--EIR 252 Query: 76 AQGEVIAP---DPASRGQAGDVFDPSVVVDPQAYVWQYPHWQGRPWEETVL------YEL 126 +G V+ P DP +R SVV D + YVWQ W + L YE+ Sbjct: 253 GRGGVLLPLKADPVARATEPPPATASVVADARPYVWQDQQWMAERAQRQSLSAPMSIYEV 312 Query: 127 HPGAMGGFAGIQQR---LGELAE--------LGITAIELMPVAEFPGAHNWGYDGVLPFA 175 HP + R ELA+ +G T IELMPV E P +WGY + FA Sbjct: 313 HPASWRRVPEQDHRNLNWDELADQLIPYVVGMGFTHIELMPVMEHPFGGSWGYQPISMFA 372 Query: 176 PDASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGNFL----------HCYAPEFFR 225 P A +G PE+ A +D H G+ V LD V HF D + L H E F Sbjct: 373 PSARFGKPERFAAFVDRCHQAGIGVLLDWVPAHFPSDQHGLAQFDGTALYEHADPREGFH 432 Query: 226 QDRQTLWGASIDFRRPEVADFFTQNALYWLQEYRFDGLRLDAVHAICEPDWLVTLGQRI 284 QD TL + R EV F +AL WL+ + DGLR+DAV ++ D+ G+ + Sbjct: 433 QDWNTL---IFNLGRNEVRGFLIASALEWLEHFHVDGLRVDAVASMLYRDYSRKEGEWV 488 >gi|300311642|ref|YP_003775734.1| 1,4-alpha-glucan branching protein [Herbaspirillum seropedicae SmR1] Length = 819 Score = 99.8 bits (247), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 97/310 (31%), Positives = 133/310 (42%), Gaps = 46/310 (14%) Query: 14 QVSAPGQVRFRVWAPGAEAVAV-----EIIGRERLPMTPEDAESPAWYVLEVPCDPGTLY 68 Q+ VRF VWAP A+ V+V + GR R PM + W + G LY Sbjct: 212 QIDGVEGVRFAVWAPNAQRVSVIGDFNQWDGR-RNPMRLRQSAG-VWELFVPRLGDGALY 269 Query: 69 RYVFQTRAQGEVI--APDPASRGQAGDVFDPSVVVDPQAYV----WQYPHW-QGRPWEET 121 +Y GE++ DP +R S V+D V WQ HW Q R +T Sbjct: 270 KYEI-IGPHGELLPGKADPMARATEVPPATASRVIDDSKLVSGRRWQDAHWLQQRAQGQT 328 Query: 122 -----VLYELHPGAMGGFAGIQQR---LGELAE--------LGITAIELMPVAEFPGAHN 165 +YE+H + G R ELA+ +G T +EL+PV E P + Sbjct: 329 RQAPMSIYEVHAASWLRLPGEGNRNLDWNELADRLIPYVKGMGFTHVELLPVMEHPFGGS 388 Query: 166 WGYDGVLPFAPDASYGTPEQLKALIDTAHGLGMMVFLDVVYNHFGPDGNFL--------- 216 WGY + FAP A +G P + +D H G+ V LD V HF D + L Sbjct: 389 WGYQPLSQFAPSARFGPPAEFATFVDACHRAGIGVLLDWVPAHFPSDPHGLAYFDGTPLY 448 Query: 217 -HCYAPEFFRQDRQTLWGASI-DFRRPEVADFFTQNALYWLQEYRFDGLRLDAVHAICEP 274 H E QD W I + R EV F +AL WL+ + DGLR+DAV ++ Sbjct: 449 EHGDPREGIHQD----WNTHIYNLGRHEVRGFLIASALEWLEHFHVDGLRVDAVASMLYR 504 Query: 275 DWLVTLGQRI 284 D+ + GQ + Sbjct: 505 DYSRSSGQWV 514 >gi|300311817|ref|YP_003775909.1| glycogen debranching enzyme protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 92.4 bits (228), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 67/210 (31%), Positives = 91/210 (43%), Gaps = 40/210 (19%) Query: 97 PSVVVDPQAYVWQYPHWQGRPWEETVLYELH------------PGAMGGFAGIQQ--RLG 142 P +V A+VW++ PW +TV+YE H P G FA + + Sbjct: 141 PKAMVTEDAFVWEHDRLPQVPWNKTVIYEAHVKGISRLREDLLPHERGTFAALADPAFID 200 Query: 143 ELAELGITAIELMPVAEF--------PGAHN-WGYDGVLPFAPDASY---GTPEQLKALI 190 L LG+T +ELMPV F G HN WGY + FAP+ Y G P +L+ + Sbjct: 201 HLLRLGVTTLELMPVHAFLQDSFLVERGLHNYWGYSTLSYFAPEPGYLSMGDPNELRMAV 260 Query: 191 DTAHGLGMMVFLDVVYNH------FGPDGNFLHCYAPEFFR----QDR----QTLWGASI 236 H G+ V LDVVYNH GP +F +FR Q+R T G ++ Sbjct: 261 RRLHSAGIEVILDVVYNHTSCGSELGPTLSFRGLDNASYFRLLPGQERYHINDTGCGNTL 320 Query: 237 DFRRPEVADFFTQNALYWLQEYRFDGLRLD 266 + P V + YW Q + DG R D Sbjct: 321 NLSHPRVLQMVLDSLRYWAQSFHIDGFRFD 350 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,201,214 Number of extensions: 101091 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 4 Length of query: 630 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 539 Effective length of database: 1,188,985 Effective search space: 640862915 Effective search space used: 640862915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)