BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2525.p (564 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 1038 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 153 3e-38 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 90 4e-19 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 1038 bits (2684), Expect = 0.0, Method: Compositional matrix adjust. Identities = 509/564 (90%), Positives = 539/564 (95%) Query: 1 MPTTVADILIDTLYQIGVRQIFGVVGDALNPFTDALRRDKRIEWIGVRHEEGAALAAAGQ 60 MP TVADILIDTLY+IGVRQIFGVVGDALNP TDA+RRDKRIEWIGVRHEEGAALAAAGQ Sbjct: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAARRGTDYLQENSPDML 120 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAA RGTDYLQENSP++L Sbjct: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 Query: 121 FRDVAAYSQIVTSPEQAPAVFHQAIAQAYAQRGVAHLNIPADIIGAKVSSTVPSIHTLRQ 180 FRDVAAYSQI+TSP+QAPAVFHQAIAQAYAQRGVAHLNIPAD+IGAK S +VPS+HTLR+ Sbjct: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 Query: 181 RAELAPPTADIAAAAELINSASSVAIFAGNGCRGAIEPVLALASKLQAPVMHTFRGKDLV 240 R L+PP ADI AAA+LIN+A SVAIFAGNGCRGAI+ VLALASKLQAPVMHTFRGKDL Sbjct: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLA 240 Query: 241 AFDHPHWIGGVGLIGGAPGTDALHGAEVMLMLGSDYPYSEFLPTQSKVIQVDERGFVLGR 300 ++DHPHWIGGVGLIGGAPGTDALH AEVMLMLGSDYPYSEFLPT+SKVIQ+DERGFVLGR Sbjct: 241 SYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 Query: 301 RAPVTLGITGSVGPTVQALLEQVEGKTDGSFLQQANAQRERWNAKLDSAAEIKRHHKKIK 360 RAPVTLGITGSV P VQ LLE+VEGKTDG+FLQQANA RERWNAKLDSAAEIKRHHKKIK Sbjct: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 Query: 361 PQSLARALSDLADDDAQFVIDTGVVTLWCGNWIRQRGQQRLLASFNNAAVGTSLGQANGI 420 PQS+ARA+SDLADDDA FV+DTGVVTLWCGNWIRQRG QR+LASFNNAAVGTSLGQANGI Sbjct: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 Query: 421 QALDRQRQVLVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF 480 QALDR+RQV+VAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF Sbjct: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF 480 Query: 481 EGSEFPNADFAAFARACGGQGFTARTPDELQDSLQQLLAASGPAVLDVFIDPAELPVMPH 540 EGSEFPN DFAAFARACGGQGF+ RTP+EL+ LQQLLAA GPAVLDVFIDP ELPVMPH Sbjct: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 Query: 541 IKMEQIWRFGLAKVKESLIAMKGG 564 IKMEQIWRFGLAKV+ESLIAMKGG Sbjct: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 153 bits (387), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 141/557 (25%), Positives = 232/557 (41%), Gaps = 24/557 (4%) Query: 4 TVADILIDTLYQIGVRQIFGVVGDALNPFTDALRRDKRIEWIGVRHEEGAALAAAGQAKL 63 T ADIL+ L + GV +FG G A+ DA+ + + + I VRHE+ A AA ++ Sbjct: 7 TGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 Query: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAARRGTDYLQENSPDMLFRD 123 + ++ V T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 67 SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRP 126 Query: 124 VAAYSQIVTSPEQAPAVFHQAIAQAYAQR-GVAHLNIPADIIGAKVSSTVPSIHTLRQRA 182 ++ +V + +A A R G ++IP DI + P +R Sbjct: 127 CVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYK 186 Query: 183 ELAP-PTADIAAAAELINSASSVAIFAGNGC--RGAIEPVLALASKLQAPVMHTFRGKDL 239 + + I A +L+ SA I+ G G A + L +L P +T G Sbjct: 187 PVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGA 246 Query: 240 VAFDHPHWIGGVGLIGGAPGTDALHGAEVMLMLGSDYPY------SEFLPTQSKVIQVDE 293 ++G G+ G A+ +V++ +G+ + F K+I +D Sbjct: 247 YRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHIDI 306 Query: 294 RGFVLGRRAPVTLGITGSVGPTVQALLEQV---EGKTDGSFL----QQANAQRERWNAKL 346 + +R V + I G+V +Q LL Q+ E + +G+ L +Q N R+R K Sbjct: 307 DPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKF 366 Query: 347 DSAAEIKRHHKKIKPQSLARALSDLADDDAQFVIDTGVVTLWCGNWIRQRGQQRLLASFN 406 ++ E IKPQS+ + + ++ DA D G +W + +R + S Sbjct: 367 ATSEEF------IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 407 NAAVGTSLGQANGIQALDRQRQVLVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWG 466 +G L A G+Q + V G+ M + E T ++ L K+++ NNR G Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 467 LV-HLEMEEAGMPAFEGSEFPNADFAAFARACGGQGFTARTPDELQDSLQQLLAASGPAV 525 +V + + G E DF A + G G P ++ +L++ A V Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 526 LDVFIDPAELPVMPHIK 542 FI V P +K Sbjct: 541 FMNFITDQTENVWPMVK 557 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 90.1 bits (222), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 123/500 (24%), Positives = 211/500 (42%), Gaps = 43/500 (8%) Query: 57 AAGQAKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAARRGTDYLQE-- 114 A +A L R+ + GPGA +L+ A + PVL + G V +RR LQ+ Sbjct: 81 AYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSRRPDPVLQQLE 140 Query: 115 --NSPDM----LFRDVAAYSQIVTSPEQAPAVFHQAIAQAY--AQRGVAHLNIPADI--I 164 N P + FR ++ + PEQ +AI AQ G L +P D+ + Sbjct: 141 DFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVTLALPQDVQTM 200 Query: 165 GAKVSSTVPSIHTLRQRAELAPPTADIAAAAELINSASSVAIFAGNGCR--GAIEPVLAL 222 S + RA+ AP ++ A L+ A + AG G A E + Sbjct: 201 AYDYPLDFFSPRVIVPRAQ-APAAQELEDAVALLRQARQPLLIAGGGVLYGNACEHLRRF 259 Query: 223 ASKLQAPVMHTFRGKDLVAFDHPHWIGGVGLIGGAPGTDAL-HGAEVMLMLGS---DYPY 278 A + PV T GK + + H +G +G + G+P +AL A+V++ +G+ D+ Sbjct: 260 AEQHGVPVAETQAGKSALPWSHALQMGAIG-VTGSPAANALAQEADVVIAVGTRLQDFTT 318 Query: 279 -SEFLPTQSKVIQVDERGFVLGRRAPVTLGITGSVGPTVQALLEQVEG-KTDGSFLQQAN 336 S L Q++++ ++ + + L +G ++AL +EG ++ + +A+ Sbjct: 319 GSHTLFAQARLLNLNVNAMDAHKWRGLALQADAGLG--LEALSHALEGWRSAPEWHARAH 376 Query: 337 AQRERWNAKL-------DSAAEIKRHHKKIKPQSLARALSDLADDDAQFVIDTGVVTLWC 389 + W ++ D+ + + I ++ R+++D D V G + Sbjct: 377 TLAQDWRERVAQITGQTDTGGRLPYDGEVIG--AIQRSVADSPSQDI-VVCAAGTLPAEL 433 Query: 390 GNWIRQRGQQRLLASFNNAAVGTSLGQANGIQALDRQRQVLVAVGDGGFTMLLGEFMTSV 449 R + + +G + G++ QR+V+V VGDG + M+ E TSV Sbjct: 434 HKLWRAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIVIVGDGSYLMMNSELATSV 493 Query: 450 EHRLPVKVVVFNNREWGLVHLEMEEAGMPAFE---------GSEFPNADFAAFARACGGQ 500 + VVV +NR +G ++ + G F G P DFAA AR+ G Sbjct: 494 MLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAGPGAPAIDFAAHARSLGAL 553 Query: 501 GFTARTPDELQDSLQQLLAA 520 G +T EL+ +LQ+ AA Sbjct: 554 GENVKTITELEAALQRARAA 573 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,669,685 Number of extensions: 67375 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 233 Number of HSP's successfully gapped: 3 Length of query: 564 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 474 Effective length of database: 1,193,720 Effective search space: 565823280 Effective search space used: 565823280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)