BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2515.p (310 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311595|ref|YP_003775687.1| two component response regulato... 485 e-138 gi|300310564|ref|YP_003774656.1| regulator of the two-component ... 224 5e-60 gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626... 69 5e-13 gi|300312232|ref|YP_003776324.1| signal transduction protein [He... 63 4e-11 >gi|300311595|ref|YP_003775687.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 485 bits (1248), Expect = e-138, Method: Compositional matrix adjust. Identities = 238/308 (77%), Positives = 266/308 (86%) Query: 1 MSCYVDHQTFHELQDSEVLPAPSGVRLKIMQMCQQEDIALSDLVAQVQGDPILAGRLIQI 60 MS YVD QTF E+QDSEVLPAPSGVRLKIMQMCQQEDIAL +LVAQVQGDP+LAGRLIQI Sbjct: 1 MSTYVDPQTFREMQDSEVLPAPSGVRLKIMQMCQQEDIALPELVAQVQGDPVLAGRLIQI 60 Query: 61 ANIPNINKGRMVAAVNIDLLLMVGLHAVRQMALAISLTESARRGDCQEFDYQRFWSRSTA 120 ANIPNINKGRMVA+VN++LLLMVGLHAVRQMALAISLTESARRGDC EFDY FWSRSTA Sbjct: 61 ANIPNINKGRMVASVNVELLLMVGLHAVRQMALAISLTESARRGDCAEFDYDAFWSRSTA 120 Query: 121 MACAGQALGELLQVAPPAEMFTVALLADIGRLGLASARPRRYGELLRSGAQGTALRAQEK 180 MACAGQALG+LLQVAPPAEMFT+ LLA IG+LGLASARP+RYGELL +GA+ LR QE+ Sbjct: 121 MACAGQALGDLLQVAPPAEMFTIGLLAGIGQLGLASARPKRYGELLLAGAKDAQLRVQER 180 Query: 181 EVFGFHHLELAAAMMQDWLFPRLFCEAVLHHGAPEQSGLDEADRQQRLIRVLELAACVAD 240 E FGF HL LAAAMMQDW FPR+FCEAVLHH P SGL+E DRQQ++ R+LELA CVAD Sbjct: 181 ESFGFDHLALAAAMMQDWHFPRIFCEAVLHHRDPAASGLEEDDRQQKVTRLLELAGCVAD 240 Query: 241 LCVASDAERTSLLPRLLRLGGPVELPISTLASVCSHISQDWSQWGALLKVPARVLPDLGT 300 +C ASDA+R +LLPRLL+LG LP+S+L VC +S DWS WGALLKVP RVL ++G Sbjct: 241 VCTASDAQRAALLPRLLQLGQHFALPVSSLEYVCGRVSWDWSDWGALLKVPTRVLREIGP 300 Query: 301 ELVAALKQ 308 EL AA +Q Sbjct: 301 ELAAAAEQ 308 >gi|300310564|ref|YP_003774656.1| regulator of the two-component system protein [Herbaspirillum seropedicae SmR1] Length = 317 Score = 224 bits (572), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 120/290 (41%), Positives = 176/290 (60%) Query: 1 MSCYVDHQTFHELQDSEVLPAPSGVRLKIMQMCQQEDIALSDLVAQVQGDPILAGRLIQI 60 M +++ F +L+ ++ LP+P GVRL IM++C Q++ +++L+ VQ DP L+GR+I+I Sbjct: 1 MLTFIEPTLFDKLRSAQALPSPGGVRLTIMRLCAQDNPDMAELIHLVQADPALSGRIIRI 60 Query: 61 ANIPNINKGRMVAAVNIDLLLMVGLHAVRQMALAISLTESARRGDCQEFDYQRFWSRSTA 120 N+ N + R +A+V++D LL+VGL AVRQ+ALA+SL E+ C F FW+ S A Sbjct: 61 VNVLNAQRVRPIASVSMDSLLLVGLQAVRQLALALSLAETRAPASCANFSLPAFWAHSFA 120 Query: 121 MACAGQALGELLQVAPPAEMFTVALLADIGRLGLASARPRRYGELLRSGAQGTALRAQEK 180 MAC+ QA+ L+VAP EMFT LLA+IG+L LASA+P Y LL A RA E+ Sbjct: 121 MACSAQAVTSQLRVAPLGEMFTTGLLANIGKLTLASAQPTGYSALLTRALDAPARRAMEQ 180 Query: 181 EVFGFHHLELAAAMMQDWLFPRLFCEAVLHHGAPEQSGLDEADRQQRLIRVLELAACVAD 240 +FGF+HL +AAA+MQDW P LFC+AVLH+ P G A RQ ++ L LA +A+ Sbjct: 181 SLFGFNHLNIAAALMQDWKIPDLFCDAVLHYETPVVIGEIAATRQDEIVWTLHLAQAMAE 240 Query: 241 LCVASDAERTSLLPRLLRLGGPVELPISTLASVCSHISQDWSQWGALLKV 290 L + + L R ++L L + QDW +W +++ Sbjct: 241 LGCGPAQQAQAALARAGTALAQLQLAPHDLLVLYQQAGQDWQEWTQTMRI 290 >gi|300311938|ref|YP_003776030.1| hypothetical protein Hsero_2626 [Herbaspirillum seropedicae SmR1] Length = 290 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 58/228 (25%), Positives = 103/228 (45%), Gaps = 9/228 (3%) Query: 19 LPAPSGVRLKIMQMCQQEDIALSDLVAQVQGDPILAGRLIQIANIPNINKGRMVAAVNID 78 LP V ++M EDI+ ++ Q+ DP L+ +++++AN + R + V+ D Sbjct: 13 LPTVPKVVQQLMASFNAEDISAHEIAQQIATDPALSAKVLRLANSAFFHASRTIGTVD-D 71 Query: 79 LLLMVGLHAVRQMALAISLTESARRGDCQEFDYQRFWSRSTAMACAGQAL---GELLQVA 135 L ++G VR + L + + + + + D +FW + A A + L ++ Sbjct: 72 ALRILGFIMVRNLVLGNGMVAAFK--NTRGMDLHQFWRHTLYTAVAARWLAMHAGMVGED 129 Query: 136 PPAEMFTVALLADIGRLGLASARPRRYGELLRSGAQGTALRAQ-EKEVFGFHHLELAAAM 194 +FTV ++ IG+L L + L R + RA+ EKE +GFH+ +++A + Sbjct: 130 NGDAVFTVGMMHGIGQLQLHAVAANAVAPLDRHSNVLDSDRAEREKEAWGFHYADVSAEL 189 Query: 195 MQDWLFPRLFCEAVLHHGAPEQSGLDEADRQQRLIRVLELAACVADLC 242 W FP A+ P+ G DR + + AA VA L Sbjct: 190 AAIWHFPDALVTAL--RSVPDPLGAGRFDRAAAWVHLGGWAARVAILS 235 >gi|300312232|ref|YP_003776324.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 274 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 45/209 (21%), Positives = 97/209 (46%), Gaps = 5/209 (2%) Query: 13 LQDSEVLPAPSGVRLKIMQMCQQEDIALSDLVAQVQGDPILAGRLIQIANIPNINKGRMV 72 Q LP+ V ++++ E +++ ++ ++ D +L+ +++++AN + R + Sbjct: 7 FQQQTALPSIPKVVQEVIESFNNESVSIEEIAKKLAADQVLSAKILRLANSSYYHVSRTI 66 Query: 73 AAVNIDLLLMVGLHAVRQMALAISLTESARRGDCQEFDYQRFWSRSTAMACAGQALGELL 132 V+ D +LM+G VR + ++ + S ++ D + FW S A + L + Sbjct: 67 GTVD-DAVLMLGFMTVRTLVVSTGMAGSFKK--LPGVDLKLFWRYSLDTALVAKWLAKRG 123 Query: 133 QVAPPAEMFTVALLADIGRLGLASARPRRYGELLRSGAQGTALRAQ-EKEVFGFHHLELA 191 ++ FTV L+ IG+L + + P + ++ + A R E++ FG+ ++ Sbjct: 124 KLNSDF-AFTVGLMHAIGQLVMHAGMPEQALQVDKVAGPLDARRLDVERKSFGYDFADVG 182 Query: 192 AAMMQDWLFPRLFCEAVLHHGAPEQSGLD 220 A + + W FP F + + P Q D Sbjct: 183 AELARRWRFPDEFAKVIEAFPQPLQGEFD 211 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 909,792 Number of extensions: 34918 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 107 Number of HSP's successfully gapped: 4 Length of query: 310 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 225 Effective length of database: 1,217,395 Effective search space: 273913875 Effective search space used: 273913875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)