BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2511.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 371 e-104 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 302 5e-83 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 296 3e-81 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 275 6e-75 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 275 7e-75 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 371 bits (953), Expect = e-104, Method: Compositional matrix adjust. Identities = 204/475 (42%), Positives = 290/475 (61%), Gaps = 7/475 (1%) Query: 8 LSGQLFAAGQFIEG-QGARLQVRNPARNAVIGQIAVATPDEIAAAVAAAVAAWRAWAGAP 66 L Q F GQ+ + QG RL V NPA ++G + + E A+ AA AAW AW Sbjct: 10 LRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKT 69 Query: 67 ARVRAEALHTLGRLILADAPAMAEVMTLEQGKPCNEALGEIKKLAEACHFYAEEAVRVHG 126 A+ R+ L L+LA+ +A +MT EQGKP EA GEI A ++AEE R +G Sbjct: 70 AKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYG 129 Query: 127 EVVPNDQPGYQSLVLREAIGVVAAITPWNYPAELVGWKLCAAVAAGCAVIIKPSEFTPFT 186 + +P+ P + +V++EAIGV AAITPWN+PA ++ K A+AAGC +++KP+E TPF+ Sbjct: 130 DTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFS 189 Query: 187 ALYIAQKVQQAGIPPGIVTVLTGDGATVGQALVAHPDIAKIAFTGSSQTG-LRIQQSCPS 245 AL +A ++AGIP G+ +V+TG +G + ++P + KI+FTGS+ G L +QQS S Sbjct: 190 ALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASS 249 Query: 246 IKRLSLELGGNCPLLVTRHADLDQAVQGAVRRSFRNMGQICIAINRIYVERVVYADFLAR 305 IK+LSLELGGN P +V ADLD AV+GA+ +RN GQ C+ NRIYV+ VY F A+ Sbjct: 250 IKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAK 309 Query: 306 FAVAARALVIGDGLSHPAADLGSMAQAAPLQKTLAHLEDALAQGARLITGGAAPAGEEFV 365 A + +G G + G + +QK H+ DA+A+GAR++ GG A + Sbjct: 310 LVEAVKKFKVGQG-TEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHA----L 364 Query: 366 NGLFFQPTIVADCTPQMRVMREETFGPLVGVAPFDSLAEALALANDSPYGLAAYVYTQDL 425 FF+PT++AD TP M+V REETFGP+ + F + E LALAND+ +GLA+Y Y++D+ Sbjct: 365 GHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDI 424 Query: 426 QEIHRCAAALDVGNVAINHVDAGIMNAPYGGRKQSGIGVEHAREGLLEYFQFKHV 480 I R A L+ G V IN AP+GG KQSG+G E + G+ +Y K++ Sbjct: 425 GRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGSHYGIDDYLVVKYL 479 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 302 bits (773), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 185/474 (39%), Positives = 275/474 (58%), Gaps = 5/474 (1%) Query: 11 QLFAAGQFIEGQGARLQVRNPARNAVIGQIAVATPDEIAAAVAAAVAAWRAWAGAPARVR 70 QL A G +L V +PA V + + AAV AAVAA+ AW PA+ R Sbjct: 16 QLIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAWRATPAKQR 75 Query: 71 AEALHTLGRLILADAPAMAEVMTLEQGKPCNEALGEIKKLAEACHFYAEEAVRVHGEVVP 130 A + L+LA + +++ EQGKP EA GE+ A ++ EEA R +G+++P Sbjct: 76 AGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIP 135 Query: 131 NDQPGYQSLVLREAIGVVAAITPWNYPAELVGWKLCAAVAAGCAVIIKPSEFTPFTALYI 190 G + + L+E +GVVAAITPWN+PA ++ K+ A+AAGC V+ KP+E TP T+L + Sbjct: 136 APVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLAL 195 Query: 191 AQKVQQAGIPPGIVTVLTGDGATVGQAL---VAHPDIAKIAFTGSSQTGLRI-QQSCPSI 246 + Q+AG+P G++ ++T + + +A + KI+FTGS+ G + + S ++ Sbjct: 196 VRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTL 255 Query: 247 KRLSLELGGNCPLLVTRHADLDQAVQGAVRRSFRNMGQICIAINRIYVERVVYADFLARF 306 K+LSLELGGN P +V AD+D A+ G + FRN GQ C++ NRIYV+ VY F+ + Sbjct: 256 KKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCVSPNRIYVQEKVYDAFVDKL 315 Query: 307 AVAARALVIGDGLSHPAADLGSMAQAAPLQKTLAHLEDALAQGARLITGGAAPAGEEFVN 366 AL +G + PA+ +G M A + K H+ DA+A+GAR+ITGG G F + Sbjct: 316 GARVAALKVGPA-TDPASQIGPMINARAIAKIDQHVRDAIARGARVITGGKRLQGPGFGS 374 Query: 367 GLFFQPTIVADCTPQMRVMREETFGPLVGVAPFDSLAEALALANDSPYGLAAYVYTQDLQ 426 ++ PT++AD T M+ EETFGP+ + F + E +A AN +P+GLAAY Y+ D++ Sbjct: 375 DNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIAAANATPFGLAAYFYSTDVR 434 Query: 427 EIHRCAAALDVGNVAINHVDAGIMNAPYGGRKQSGIGVEHAREGLLEYFQFKHV 480 IHR AL+ G V +N AP+GG K+SG G E + GL +Y K+V Sbjct: 435 RIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGSVHGLDDYLHTKYV 488 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 296 bits (757), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 173/472 (36%), Positives = 260/472 (55%), Gaps = 7/472 (1%) Query: 11 QLFAAGQFI-EGQGARLQVRNPARNAVIGQIAVATPDEIAAAVAAAVAAWRAWAGAPARV 69 QLF G++ G + V NPA VIG+IA A ++ A+ AA + W A Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 70 RAEALHTLGRLILADAPAMAEVMTLEQGKPCNEALGEIKKLAEACHFYAEEAVRVHGEVV 129 R++ + L+ A +A +MT+EQGKP EA E A+ ++AEEA R +G +V Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 130 PNDQPGYQSLVLREAIGVVAAITPWNYPAELVGWKLCAAVAAGCAVIIKPSEFTPFTALY 189 P PG +V++E +G VAA TPWN+P V KL AA+AAGC++I+K E TP + Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 190 IAQKVQQAGIPPGIVTVLTGDGATVGQALVAHPDIAKIAFTGSSQTGLRIQQ-SCPSIKR 248 + + AG+P G++ ++ G A + + L+ HP I KI+FTGS+ G ++ + +KR Sbjct: 186 LIRAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKR 245 Query: 249 LSLELGGNCPLLVTRHADLDQAVQGAVRRSFRNMGQICIAINRIYVERVVYADFLARFAV 308 ++ELGG+ P +V AD+D A + FRN GQ+C++ R V++ V+ F+ +F Sbjct: 246 ATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVT 305 Query: 309 AARALVIGDGLSHPAADLGSMAQAAPLQKTLAHLEDALAQGARLITGGAAPAGEEFVNGL 368 A L +G+GL +G +A + A ++DA++QGA+L TGG + G Sbjct: 306 YAEQLKVGNGL-EAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNK----GY 360 Query: 369 FFQPTIVADCTPQMRVMREETFGPLVGVAPFDSLAEALALANDSPYGLAAYVYTQDLQEI 428 FFQPT++ + Q R M EE FGPL + F++L E + AN PYGLAAY + + Sbjct: 361 FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTK 420 Query: 429 HRCAAALDVGNVAINHVDAGIMNAPYGGRKQSGIGVEHAREGLLEYFQFKHV 480 H AA ++ G + INH+ + P+GG K SG G E E + Y K V Sbjct: 421 HALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGGTEAIEAYINTKLV 472 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 275 bits (703), Expect = 6e-75, Method: Compositional matrix adjust. Identities = 185/480 (38%), Positives = 260/480 (54%), Gaps = 17/480 (3%) Query: 11 QLFAAGQFIE-GQGARLQVRNPARNAVIGQIAVATPDEIAAAVAAAVAAWR--AWAGAPA 67 Q F AG+ E G R+ + NP + A T ++A AVAAA A AW+ Sbjct: 6 QHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSG 65 Query: 68 RVRAEALHTLGRLILADAPAMAEVMTLEQGK-PCNEALGEIKKLAEACHFYAEEAVRVHG 126 R + LH L L+ D +A++ G+ P + ++ A A ++ G Sbjct: 66 LQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEG 125 Query: 127 EVVPNDQPGYQ-----SLVLREAIGVVAAITPWNYPAELVGWKLCAAVAAGCAVIIKPSE 181 +P GY S +RE IGVV AI PWN P + WK+ A +AAGC V+IKP+E Sbjct: 126 RTIPT--AGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAE 183 Query: 182 FTPFTALYIAQKVQQAGIPPGIVTVLTGDGATVGQALVAHPDIAKIAFTGSSQTGLRIQQ 241 TP +AL++A+ Q+AG P G++ V+TG G +VG+AL HPD+AKI+FTGS + G IQ+ Sbjct: 184 ETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQR 243 Query: 242 -SCPSIKRLSLELGGNCPLLVTRHADLDQAVQGAVRRSFRNMGQICIAINRIYVERVVYA 300 + KR++LELGG P +V A + A+ G F N GQ+C A +RI V+R + Sbjct: 244 IAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQ 303 Query: 301 DFLARFAVAARALVIGDGLSHPAADLGSMAQAAPLQKTLAHLEDALAQGARLITGGAAPA 360 F A AA + +GD S P +G +A+ A ++ +++ L +GA L+ GG + Sbjct: 304 RFAKALAEAAAGITVGD-PSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVSRP 362 Query: 361 GEEFVNGLFFQPTIVADCTPQMRVMREETFGPLVGVAPFDSLAEALALANDSPYGLAAYV 420 + G F QPTI AD M + REE FGP+ + FD+ EA+ALANDS YGLAA V Sbjct: 363 DK----GWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATV 418 Query: 421 YTQDLQEIHRCAAALDVGNVAINHVDAGIMNAPYGGRKQSGIGVEHAREGLLEYFQFKHV 480 +T +L HR AA + VG V +N N P+GG K SG+G E G L Y + K V Sbjct: 419 WTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTV 478 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 275 bits (702), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 171/449 (38%), Positives = 256/449 (57%), Gaps = 9/449 (2%) Query: 11 QLFAAGQFIEGQGARLQ-VRNPARNAVIGQIAVATPDEIAAAVAAAVAAWRAWAGAPARV 69 QL+ G+F +G R Q VR+PA V+G++ A +I A+ AA A++ W Sbjct: 6 QLYIDGRFHGVEGRRYQEVRDPATGDVLGRLPWAERADIEEAIQAAHRAFQQWRQVSPLE 65 Query: 70 RAEALHTLGRLILADAPAMAEVMTLEQGKPCNEALGEIKKLAEACHFYAEEAVRVHGEVV 129 R++ L L A AMA +T++ GKP +A+ EI AE ++AEE R++G VV Sbjct: 66 RSDILRRAAALARERAQAMAPAITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVV 125 Query: 130 PNDQPGYQSLVLREAIGVVAAITPWNYPAELVGWKLCAAVAAGCAVIIK-PSEFTPFTAL 188 P + LV+RE +GV AAI+PWN+P K+ AA+AAGC V++K PSE +P + Sbjct: 126 GARHPEVRQLVVREPVGVCAAISPWNFPFGQAMKKVAAAMAAGCTVVLKGPSE-SPAAIV 184 Query: 189 YIAQKVQQAGIPPGIVTVLTGDGATVGQALVAHPDIAKIAFTGSSQTGLRI-QQSCPSIK 247 +AQ AG+P G+ +++G + + Q L+ P + I+FTGS G ++ Q+ +K Sbjct: 185 LMAQLFHDAGVPAGVFNIVSGVSSEISQHLIESPLVRNISFTGSVPVGSKLAAQAALHMK 244 Query: 248 RLSLELGGNCPLLVTRHADLDQAVQGAVRRSFRNMGQICIAINRIYVERVVYADFLARFA 307 R+S+ELGG+ P+LV A+++ A + R RN GQ+C++ +R YV++ +YA F+ FA Sbjct: 245 RMSMELGGHAPVLVFDDAEVEPAARLLARLKTRNAGQVCVSPSRFYVQKGIYARFVTAFA 304 Query: 308 VAARALVIGDGLSHPAADLGSMAQAAPLQKTLAHLEDALAQGARLITGGAAPAGEEFVNG 367 A + +G G P +G + + ++DALAQG+ L+TGGA G+ G Sbjct: 305 EALLQIKVGPG-HEPGVQMGPLIHGRRFEAVQGLIDDALAQGSELVTGGAR-LGQ---RG 359 Query: 368 LFFQPTIVADCTPQMRVMREETFGPLVGVAPFDSLAEALALANDSPYGLAAYVYTQDLQE 427 F+ PT++ P RVM+EE FGP+ V PF EA+A+AN P GLA+YV+TQ L Sbjct: 360 HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIAMANALPLGLASYVFTQSLDT 419 Query: 428 IHRCAAALDVGNVAINHVDAGIMNAPYGG 456 HR + L G V INH P+GG Sbjct: 420 AHRVSRDLQAGMVNINHSGMAHPELPFGG 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,424,526 Number of extensions: 58364 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 21 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)