BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2503.p (564 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate dehydro... 768 0.0 gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 61 2e-10 gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 60 4e-10 >gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 768 bits (1982), Expect = 0.0, Method: Compositional matrix adjust. Identities = 372/557 (66%), Positives = 441/557 (79%), Gaps = 1/557 (0%) Query: 7 LIEGLCEIVGQAHVLTSAAAKRRYCRGYRYGRGEALAVVRPGSLRQMWQVLQACHQADVI 66 +I L IVG HVLT A RR+ +G RYG GE LAVVRPG L +MW+VLQ CH A+++ Sbjct: 14 VISKLEAIVGSKHVLTGDAQTRRFRQGLRYGGGEVLAVVRPGRLLEMWRVLQVCHAANMV 73 Query: 67 VICQAANTGLTGGSTPVEEGYDRPVIVLNTLRLRRLYLLDQGRQVLTQPGVTLDQLERRL 126 +ICQAANTGLTGGSTP+ EGYDRPV+++NT+RL RL LLDQG+QVL+QPGVTLDQLERRL Sbjct: 74 IICQAANTGLTGGSTPIAEGYDRPVVLINTMRLTRLELLDQGQQVLSQPGVTLDQLERRL 133 Query: 127 KPLGREPHSVIGSSCMGASVTGGICNNSGGSLIRRGPAYTQLALYAQVDAQGRLQLVNHL 186 KPLGREPHSVIGSSC+GAS+TGGICNNSGGSLI+RGPAYTQ AL+A++D QGRL+LVNHL Sbjct: 134 KPLGREPHSVIGSSCIGASITGGICNNSGGSLIQRGPAYTQYALFARIDEQGRLELVNHL 193 Query: 187 GLGLGDTPEEILGRLDRGELPEGAGACDCGGRHASDQEYGQHVRDVDAPTPARYNADPRR 246 G+ LG+ PE IL +LD G + + A G+ ASD+EY HVRD+ +P PAR+NADPRR Sbjct: 194 GIDLGEDPERILQQLDAGHI-DPALVHHVPGQMASDREYADHVRDISSPVPARFNADPRR 252 Query: 247 LYEASGSAGHVVVFALRLDTFPADDAPTVFYIGSNDPAELEAIRRTLLQHAVHLPIAGEY 306 LYEASGSAG + VFALRLDTF AD VFYIG+NDPA LE +RR +L + LPIAGEY Sbjct: 253 LYEASGSAGKIAVFALRLDTFAADQVTQVFYIGANDPAVLEDLRRHILSNFRALPIAGEY 312 Query: 307 MHRSAFEASERYGKDLFLFIQRFGTERLMQAYALKSRVDAWLERLGLRAGTSDRILQRLA 366 MHRS ++ + +YGKDLFL+I++ GT+++ A+ALKSR DA E+LGL SDR+LQ LA Sbjct: 313 MHRSGYDLARKYGKDLFLYIRKAGTDKVPLAFALKSRFDALTEKLGLGRSLSDRLLQALA 372 Query: 367 HWLPPHLPPRLVVYRDGYEHHLLLKMSQQGADYARGVLQARFPSASGDFFECEAGEGQAA 426 LP HLPPRL RD Y HHLLLKM +G + AR L A P+A F EC+A E AA Sbjct: 373 DRLPAHLPPRLEDMRDRYAHHLLLKMGDEGIEEARAYLHAILPTAQAGFIECDAEEAGAA 432 Query: 427 FLNRFVVGAAIVRYRAVHEREVEDIVALDIALPRNTLDWFERLPPEIADKIRFTMYCGHF 486 FLNRF +G A RYRAVH+ VEDIVALD+ALPRNT DWFE LP E+A++I FTMYCGHF Sbjct: 433 FLNRFAIGNAANRYRAVHQDTVEDIVALDVALPRNTYDWFEVLPKELAEQIEFTMYCGHF 492 Query: 487 FCHVMHQEYLVNKGEDCETLKQAILALLEAKGAKYPAEHNVGHQYRAEPALEAFYRRLDP 546 FCHVMHQEYLV KG DC+ +KQ +LA+LEA+GA+YPAEHN GH Y A P +E FYRRLDP Sbjct: 493 FCHVMHQEYLVKKGRDCDAVKQRLLAMLEARGARYPAEHNFGHLYHAGPEVEQFYRRLDP 552 Query: 547 TNSFNPGIGKTSRRKHW 563 TNSFNPGIG TS R+HW Sbjct: 553 TNSFNPGIGLTSTRQHW 569 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 57/172 (33%), Positives = 85/172 (49%), Gaps = 13/172 (7%) Query: 15 VGQAHVLTSAAAKRRYCRGYRYGR--GEALAVVRPGSLRQMWQVLQACHQADVIVICQAA 72 +G HVLT A Y R GR G ALAV+RP ++ V++ C Q V ++ Q Sbjct: 19 IGAPHVLTDAHDTAGYLTDQR-GRYTGRALAVLRPADSAEVAAVVRLCAQFAVPLVPQGG 77 Query: 73 NTGLTGGSTPVEEGYDRPVIVLNTLRLRRLYLLDQGRQVLT-QPGVTLDQLERRLKPLGR 131 NTGL GS P ++G +VL+ RL R+ +D +T + G L L+ + GR Sbjct: 78 NTGLVLGSVPDQQGN---AVVLSLRRLNRIRAVDALNNTITVESGCVLQHLQEQAAAAGR 134 Query: 132 E-PHSVIGS-SCMGASVTGGICNNSGGSLIRRGPAYTQLALYAQ-VDAQGRL 180 P S+ SC ++ G + N+GG+ + R +L L + V AQG + Sbjct: 135 LFPLSLAAEGSC---TIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEI 183 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 60.1 bits (144), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 43/152 (28%), Positives = 75/152 (49%), Gaps = 8/152 (5%) Query: 7 LIEGLCEIVGQAHVLTSAAAKRRY-CRGYRYGRGEALAVVRPGSLRQMWQVLQACHQADV 65 +++ L +V VL R Y C G R + VV P + Q+ +++ CH V Sbjct: 17 VVDALRRVVPAHCVLCDEEDTRPYECDGLAAYRQLPMVVVLPENEAQVVAIMKTCHALQV 76 Query: 66 IVICQAANTGLTGGSTPVEEGYDRPVIVLNTLRLRRLYLLDQ-GRQVLTQPGVTLDQLER 124 ++ + A TGL+GG+TP+ +G +V++T + ++ +D+ R + QPGV + Sbjct: 77 QIVPRGAGTGLSGGATPIADG-----VVVSTAKFNQILQMDKYSRTAVVQPGVRNLAISE 131 Query: 125 RLKPLGREPHSVIGSSCMGASVTGGICNNSGG 156 P G ++ SS + S+ G + NSGG Sbjct: 132 AAAPHGLY-YAPDPSSQIACSIGGNVAENSGG 162 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,888,196 Number of extensions: 85757 Number of successful extensions: 250 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 3 Length of query: 564 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 474 Effective length of database: 1,193,720 Effective search space: 565823280 Effective search space used: 565823280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)