BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2501.p (873 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 1764 0.0 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 684 0.0 gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 78 3e-15 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 1764 bits (4570), Expect = 0.0, Method: Compositional matrix adjust. Identities = 860/873 (98%), Positives = 863/873 (98%) Query: 1 MNTQYRKPLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDSLRQ 60 MNTQYRK LPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDSLRQ Sbjct: 1 MNTQYRKQLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDSLRQ 60 Query: 61 LIERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQLIVDH 120 LIERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQLIVDH Sbjct: 61 LIERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQLIVDH 120 Query: 121 SLAVECGGDDPQAFEKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQINLEKM 180 SLAVECGGDDPQAF KNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQINLEKM Sbjct: 121 SLAVECGGDDPQAFVKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQINLEKM 180 Query: 181 SPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGV 240 SPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGV Sbjct: 181 SPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGV 240 Query: 241 ELTGRRQSGITATDIVLSLTEFLRKQKVVGAYLEFYGEGAASLTLGDRATISNMAPEYGA 300 ELTGRRQ GITATDIVLSLTEFLRKQKVVGAYLEFYG+GAASLTLGDRATISNMAPEYGA Sbjct: 241 ELTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMAPEYGA 300 Query: 301 TAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKAEYERVLRFDLSTVVR 360 TAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAK EYERVLRFDLSTVVR Sbjct: 301 TAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKVEYERVLRFDLSTVVR 360 Query: 361 TLAGPSNPHKRLPVSELAAQGIAGKVEKEPGKMPDGAVIIAAITSCTNTSNPRNVIAAAL 420 TLAGPSNPHKRLPVSELAAQGIAGKVE EPGKMPDGAVIIAAITSCTNTSNPRNVIAA L Sbjct: 361 TLAGPSNPHKRLPVSELAAQGIAGKVENEPGKMPDGAVIIAAITSCTNTSNPRNVIAAGL 420 Query: 421 LARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSG 480 LARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSG Sbjct: 421 LARNANRLGLARKPWVKSSLAPGSKAVELYLEEAGLTADLEKLGFGIVAFACTTCNGMSG 480 Query: 481 ALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE 540 ALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE Sbjct: 481 ALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE 540 Query: 541 QDVLGVTAEGREIRLKDIWPSDEEIDAVVAAAVKPQQFRNVYTPMFAARVDRSQSVSPLY 600 QDVLGVTAEGREIRLKDIWPSDEEIDAVV AAVKPQQFRNVYTPMFAARVDRSQSVSPLY Sbjct: 541 QDVLGVTAEGREIRLKDIWPSDEEIDAVVTAAVKPQQFRNVYTPMFAARVDRSQSVSPLY 600 Query: 601 DWRAQSTYIRRPPYWEGALAGARTLTGMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLA 660 DWR QSTYIRRPPYWEGALAG RTL+GMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLA Sbjct: 601 DWRPQSTYIRRPPYWEGALAGERTLSGMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLA 660 Query: 661 KMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKKADGSVQQGSLARLEPEGRVMR 720 KMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKKADGSVQQGSLARLEPEG+VMR Sbjct: 661 KMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKKADGSVQQGSLARLEPEGKVMR 720 Query: 721 MWETIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGM 780 MWETIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGM Sbjct: 721 MWETIETYMERKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGM 780 Query: 781 GVLPLEFKPGENRNTYAIDGTETYDVIGQRRPRADLTLVIHRKNGERVEVPVTCRLDTAE 840 GVLPLEFK GENRNTYAIDGTETYDVIGQRRPRADLTLVIHRKNGERVEVPVTCRLDTAE Sbjct: 781 GVLPLEFKAGENRNTYAIDGTETYDVIGQRRPRADLTLVIHRKNGERVEVPVTCRLDTAE 840 Query: 841 EVSIYEAGGVLQRFAQDFLEASANAGQNAKEQA 873 EVSIYEAGGVLQRFAQDFLEASANAGQNAKEQA Sbjct: 841 EVSIYEAGGVLQRFAQDFLEASANAGQNAKEQA 873 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 684 bits (1764), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/900 (42%), Positives = 545/900 (60%), Gaps = 41/900 (4%) Query: 1 MNTQYRKPLPGTRLDYFDARAAVEAIRPGAWDTLPYTSRVLAENLVRRCDPATLTDS-LR 59 +NT + G++ F + A++ LP + R++ E+++R CD +T+ ++ Sbjct: 6 LNTLKEFKISGSKKGKFYSLPALQKSLGVNISRLPVSIRIVLESVLRNCDGQKVTEEHVK 65 Query: 60 QLI----ERKRELDFPWFPARVVCHDILGQTALVDLAGLRDAIADMGGDPAKVNPVVPVQ 115 QL + R + P+ ARVV D G L DLA +R+ + +G +P + P+VPV Sbjct: 66 QLANWGAKAARVDEIPFVVARVVLQDFTGVPLLADLAAMRNVASKLGKNPKNIEPLVPVD 125 Query: 116 LIVDHSLAVECGGDDPQAFEKNRAIEDRRNEDRFHFIDWTKLAFENVDVIPPGNGIMHQI 175 L+VDHS+ ++ + +A + N +E RN +R+ F+ W AF+ V+PPG GI+HQ+ Sbjct: 126 LVVDHSVQIDHF-REKKALDLNMKLEFSRNNERYQFMKWGMQAFDTFGVVPPGFGIVHQV 184 Query: 176 NLEKMSPVIHAKDGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLP 235 NLE ++ +H K GV +PDTLVGTDSHT ++ +GV+ GVGG+EAE MLG+ + P Sbjct: 185 NLEYLARGVHNKTGVYYPDTLVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTP 244 Query: 236 DIIGVELTGRRQSGITATDIVLSLTEFLRKQKVVGAYLEFYGEGAASLTLGDRATISNMA 295 D++GV LTG + G+TATD+VL++TE LR+ KVVG ++EF+GEG SL+L DRATI+NMA Sbjct: 245 DVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGTESLSLTDRATIANMA 304 Query: 296 PEYGATAAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLA-KAEYERVLRFD 354 PEYGAT F +D TI+Y K TGR ++ E Y K L+ A + +Y + + D Sbjct: 305 PEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGVPKAGQIDYTQEVSLD 364 Query: 355 LSTVVRTLAGPSNPHKRLPVSEL------------AAQGIAGKVEK--------EPGKMP 394 L TV +LAGP P R+ + + A G K E + + Sbjct: 365 LGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAEDLGASYKNADGVDLH 424 Query: 395 DGAVIIAAITSCTNTSNPRNVIAAALLARNANRLGLARKPWVKSSLAPGSKAVELYLEEA 454 +G V+IAAITSCTNTSNP ++AA LLA+ A GL P +K+SLAPGS+ V YLE A Sbjct: 425 NGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTSLAPGSRVVTKYLEAA 484 Query: 455 GLTADLEKLGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIHPY 514 GL LEKLGFG+ A+ CTTC G +G L P + + I+ D+ + AVLSGNRNF+ RIHP Sbjct: 485 GLLPYLEKLGFGVTAYGCTTCIGNAGDLTPAMNEAIVKNDVVAAAVLSGNRNFEARIHPN 544 Query: 515 AKQAFLASPPLVVAYAIAGTIRFDIEQDVLGVTAEGREIRLKDIWPSDEEIDAVVAAAVK 574 + FLASPPLVVAYAIAG + D+ + +G G++I L DIWP+ +EI+ ++ A+ Sbjct: 545 IRANFLASPPLVVAYAIAGNVTRDLMTEPVGKGKGGKDIYLGDIWPTSQEIEKLLKFAMN 604 Query: 575 PQQFRNVYTPMFAA-----RVDRSQSVSPLYDWRAQSTYIRRPPYWEG----ALAGARTL 625 + F+ Y + A + + +Y+W + STYI PP++E A A + Sbjct: 605 AKTFKENYADVKGAPGKLWEAIKGVAKGEVYNWPS-STYIAEPPFFENFSEEPKAAAAGI 663 Query: 626 TGMRPLAVLGDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRA 685 TG R L V GD+ITTDH+SP+ +I S AG++L G+ + DFNSY + RG+H R Sbjct: 664 TGARALGVFGDSITTDHISPAGSIKESSPAGKWLQANGVLKADFNSYGSRRGNHEIMMRG 723 Query: 686 TFANPKLFNEMV-KKADGSVQQGSLARLEPEGRVMRMWETIETYMERKQPLIIIAGADYG 744 TFAN ++ N M+ K DGS +G + +P G M +++ Y+ P ++ G +YG Sbjct: 724 TFANVRIKNLMIPAKEDGSRVEGGITIHQPSGEEMSIYDAAMKYVAEGTPTMVFGGEEYG 783 Query: 745 QGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFKPGENRNTYAIDGTETY 804 GSSRDWAAKG +L GV+A++A FERIHR+NL+GMGVLPL+F ++ + I G ET+ Sbjct: 784 TGSSRDWAAKGTQLLGVKAVIARSFERIHRSNLVGMGVLPLQFLGNDSVQSLGITGDETF 843 Query: 805 DVI---GQRRPRADLTLVIHRKNGERVEVPVTCRLDTAEEVSIYEAGGVLQRFAQDFLEA 861 D+ G+ +P+ + TLVIHRKNGE EV + R+DT EV Y+ GG+L + L A Sbjct: 844 DLKGLEGEIKPQQEATLVIHRKNGETKEVKLLLRIDTPIEVDYYKHGGILPFVLRQLLAA 903 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 77.8 bits (190), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 99/358 (27%), Positives = 141/358 (39%), Gaps = 61/358 (17%) Query: 183 VIHAKDGVAFPD-TLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGVE 241 VI + G P T+V DSHT A +A G+G E E+V+ + R + V+ Sbjct: 110 VIGPEQGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQ 169 Query: 242 LTGRRQSGITATDIVLSLTEFLRKQKVVGAYLEFYGEGAASLTLGDRATISNMAPEYGAT 301 + G +G+TA DIVL++ + G +EF G SL++ R T+ NMA E GA Sbjct: 170 VDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGAR 229 Query: 302 AAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKAEYERVLRFD----LST 357 A M + D T++YLK GR ++ W ++ Y R L D Sbjct: 230 AGMVAFDDTTLEYLK--GRP---------FSPSGPHWERAVS---YWRSLHSDPGAKFDM 275 Query: 358 VVRTLAGPSNPHKRLPVSELAAQGIAGKV-----EKEPGK------------------MP 394 VV A P S + +V EK+P K + Sbjct: 276 VVTLNAAEIKPQVTWGTSPEMVVAVDARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIE 335 Query: 395 DGAVIIAAITSCTNTSNPRNVIAAALLARNANRLGLARKPWVKSSL-APGSKAVELYLEE 453 D + I SCTN S ++ AA + R G R VK ++ PGS V+ E Sbjct: 336 DIRIDKVFIGSCTN-SRIEDLREAAKVVR-----GKFRASNVKLAMVVPGSGLVKEQAER 389 Query: 454 AGLTADLEKLGFGIVAFACTTCNGMSG-ALDPKIQQEIIDRDLYSTAVLSGNRNFDGR 510 GL GF C+ C M+ L+P + NRNF+GR Sbjct: 390 EGLDKIFRDAGFEWREPGCSMCLAMNADRLEPG-----------ERCASTSNRNFEGR 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,795,041 Number of extensions: 121994 Number of successful extensions: 345 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 3 Length of query: 873 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 779 Effective length of database: 1,174,780 Effective search space: 915153620 Effective search space used: 915153620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)