BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2494.p (481 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 666 0.0 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 66 6e-12 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 65 9e-12 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 666 bits (1718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/482 (73%), Positives = 386/482 (80%), Gaps = 1/482 (0%) Query: 1 MASLCEQKPQHQTEQ-DTAQAARALFCPLALLPQGWCRDVLLRWDASGSLVEVQTDSQRG 59 MAS +Q H + + A A ALFCPLALLPQGW DVLLRWD +G L V DS RG Sbjct: 1 MASHGDQDLHHLDQAPENACANGALFCPLALLPQGWRSDVLLRWDGAGRLTHVTPDSARG 60 Query: 60 QAGLAAGPVIAGMPNLHSHAFQRAMAGLTEVMGSPSDSFWSWRKLMYRFAQRLQPEHQEA 119 A LA+GPV+ GMPNLHSHAFQRAMAGL E MGSP+DSFWSWR+LMYRFAQRLQP EA Sbjct: 61 SAALASGPVLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEA 120 Query: 120 IARHLYIEMLKAGYTSVCEFHYLHHAPGGVPYADRAELALCLMQAASQAGIGMTLLPVLY 179 IAR YIEMLKAGYTSVCEFHYLHHAP G PYAD AE+A L+QAA Q+GIGMTLLPVLY Sbjct: 121 IARQTYIEMLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTLLPVLY 180 Query: 180 QYGGFGSQPPSGEQARFIGSAQSLLALRERLLQALPENPMRRYGVAPHSLRAVSPAGLDE 239 Q GG G +P S EQ RF+ + LL LRERLLQ +PEN MRRYGVAPHSLRAV+P GL+E Sbjct: 181 QQGGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRRYGVAPHSLRAVAPTGLEE 240 Query: 240 LVQGLRRQSGGQDAPIHIHIAEQWREVQDCLAWCGQRPVQWLLSLQQLDARWCLIHATHM 299 L+ GL Q GG DAPIHIH+AEQ REV+DCLAW GQRPVQWLLS Q +DARWCL+HATHM Sbjct: 241 LLSGLAGQPGGSDAPIHIHVAEQLREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATHM 300 Query: 300 DQGEYQALAASGAVVGLCPTTEANLGDGVIEAGQLLQSATPWGIGSDSNIAINLRSELRL 359 D EY+A+A SGAVVGLCPTTEANLGDGVI+A +L+ SA WGIGSDSNIAI+LR+ELRL Sbjct: 301 DDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSDSNIAISLRAELRL 360 Query: 360 LEYGQRLHYRRRNVLASPAEPATADRLFAQAVAGGAAASGRPVAGLAPGQRADFVVLDGD 419 LEY QRLH+RRRNVLA PA ADRLF QA+AGGA A+GR VAGL PGQRAD VVLD D Sbjct: 361 LEYAQRLHHRRRNVLAVIDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVVLDPD 420 Query: 420 DVNLADRSPAQLLSALVFCEHDGQPIRDVYVGGRQVVAAGHHPLQESAREAYRQAVADLL 479 VNLA RS QLLSALVFCEHDG I DVYVGGRQVV G H L + ARE YR A+A LL Sbjct: 421 HVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRHALAALL 480 Query: 480 KD 481 KD Sbjct: 481 KD 482 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 65.9 bits (159), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 112/432 (25%), Positives = 178/432 (41%), Gaps = 50/432 (11%) Query: 74 NLHSHAFQRAMAGLTEVMGSPSDSFWSWRKLM-YRFAQRLQPEHQEAIARHLYIEMLKAG 132 N H H FQ + G + +G + + W YR+ + AR +E++++G Sbjct: 63 NTHHHLFQSLLKG--DPLGINA-TLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSG 119 Query: 133 YTSVCEFHYLHHAPGGVPYADRAELALCLMQAASQAGIGMTLL-PVLYQYGGFGSQPPSG 191 ++ + +YL++ G+P+ D +E+ L + A Q G+ L + ++ S Sbjct: 120 CGTIADHNYLYYP--GMPF-DTSEI---LFEEAQQLGLRFVLCRGTATRTRQLEAELSSA 173 Query: 192 EQARFI-GSAQSLLALRERLLQALPENPMRRYGVAPHS-LRAVSPAGLDELVQGLRRQSG 249 + + G + L R P + MRR +AP + L + +P L E+ R Sbjct: 174 LRPETLDGFLADMQGLAGRFHDPAPAS-MRRVVMAPTTPLYSAAPDELREMAVAAREMG- 231 Query: 250 GQDAPIHIHIAEQWREVQDCLAWCGQRPVQWLLSLQQLDARWCLIHATHMDQGEYQALAA 309 +H H++E A G RPV++ + L H +D E Q L Sbjct: 232 ---LRLHSHLSETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGG 288 Query: 310 SGAVVGLCPTTEANLGDGVIEAGQLLQSATPWGIGSD---SNIAINLRSELRLLEYGQRL 366 +G + CP + LG G+ L + P IG D SN A ++ SE QR Sbjct: 289 TGTGIAHCPQSNGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRA 348 Query: 367 HYRRRNVLASP----------AEPATADRLFAQAVAGGAAASG-RPVAGLAPGQRADFVV 415 RR A+P A+ AT + + GGA G + GL GQ+AD + Sbjct: 349 ---RRGQEATPSFRGGHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIAL 405 Query: 416 LDGDD---VNLADRSPAQLLSALVFCEHDGQP-IRDVYVGGRQVVAAGHHP---LQESAR 468 DD L D + + S G+P ++ ++VGGR VV P L E Sbjct: 406 YRLDDPRYFGLHDPAIGPVASG-------GRPFLKAMWVGGRAVVREDAIPGVDLAELGA 458 Query: 469 EAYRQAVADLLK 480 +A RQAV +LK Sbjct: 459 QA-RQAVKKMLK 469 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 92/385 (23%), Positives = 149/385 (38%), Gaps = 49/385 (12%) Query: 68 VIAGMPNLHSHAFQRAMAGLTEVMGSPSD-SFWSWRKLMYRFAQRLQPEHQEAIARHLYI 126 V+ G+ N H H +Q LT + S + ++W +Y L PE + Sbjct: 64 VLPGLINTHHHMYQ----SLTRAIPSAQNGELFNWLTKLYPIWAGLTPEMIQVSTLTAMA 119 Query: 127 EMLKAGYTSVCEFHYLHHAPGGVPYADRAELALCLMQAASQAGIGMTL------LPVLYQ 180 E++ +G T+ + Y++ P D E A A IGM + V Sbjct: 120 ELILSGCTTSSDHLYIY--PNQTRLDDSLEAA---------ARIGMRFHGARGAMSVGQS 168 Query: 181 YGGFGSQPPSGEQARFIGSAQSLLALRERLLQALPEN---PMRRYGVAPHSLRAVSPAGL 237 GG PP R + ++L +RL++ ++ M+R VAP S +VS + Sbjct: 169 KGGL---PPD----RVVQDEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELM 221 Query: 238 DELVQGLRRQSGGQDAPIHIHIAEQWREVQDCLAWCGQRPVQWLLSLQQLDARWCLIHAT 297 E L R G +H H+AE ++ P ++ + H Sbjct: 222 REAAD-LARHYG---VSLHTHLAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCV 277 Query: 298 HMDQGEYQALAASGAVVGLCPTTEANLGDGVIEAGQLLQSATPWGIGSD---SNIAINLR 354 +D A +G V CP + L G+ ++L + P G+G D SN + ++ Sbjct: 278 QLDAHGIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHML 337 Query: 355 SELRLLEYGQRLHYRRRNVLASPAEPATADRLFAQAVAGGAAASGR-PVAGLAPGQRADF 413 E+R QR+ + + TA + A GGA R + L PG AD Sbjct: 338 GEVRQAMLLQRVGF--------GPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADV 389 Query: 414 VVLDGDDVNLADRSPAQLLSALVFC 438 V+ D + A + ++ALVFC Sbjct: 390 VMFDTRQIAFAG-ALHDPVAALVFC 413 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,565,468 Number of extensions: 68667 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 3 Length of query: 481 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 392 Effective length of database: 1,198,455 Effective search space: 469794360 Effective search space used: 469794360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)