BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2483.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum se... 551 e-158 gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum sero... 159 3e-40 gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family ... 61 1e-10 >gi|300311612|ref|YP_003775704.1| sugar kinase [Herbaspirillum seropedicae SmR1] Length = 299 Score = 551 bits (1419), Expect = e-158, Method: Compositional matrix adjust. Identities = 287/299 (95%), Positives = 291/299 (97%) Query: 1 MIVIIGSVNMDLVLRVPRMPMPGETLAGDKFMTIPGGKGANQAVACARLAAPGTAVAMVA 60 MIVIIGSVNMDLVLRVPRMP+PGETLAGD+FMTIPGGKGANQAVACARLAAPGT VAMVA Sbjct: 1 MIVIIGSVNMDLVLRVPRMPLPGETLAGDRFMTIPGGKGANQAVACARLAAPGTRVAMVA 60 Query: 61 CVGDDAFGAQMRHSITACGIDDRYIDEVAGEATGIASIMVDAQAQNSIVIAAGANGRLDV 120 CVGDDAFG QMR SITACGIDDRYIDEVAGEATGIASIMVDA AQNSIVIAAGANGRLDV Sbjct: 61 CVGDDAFGGQMRQSITACGIDDRYIDEVAGEATGIASIMVDANAQNSIVIAAGANGRLDV 120 Query: 121 ERIERARPLIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRELLQKID 180 ERIERAR LIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPR LLQKID Sbjct: 121 ERIERARALIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRALLQKID 180 Query: 181 YLILNEIEAAMLAEQQSEDIPLLADKLHALGARNVVVTLGEKGVYGSFADGQQRHLPARK 240 YLILNEIEAAMLAE+QSEDIP+LA KLH LGARNVVVTLGEKGVYGSFADGQQRHLPARK Sbjct: 181 YLILNEIEAAMLAEEQSEDIPMLARKLHDLGARNVVVTLGEKGVYGSFADGQQRHLPARK 240 Query: 241 VQAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEVVL 299 VQAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEVVL Sbjct: 241 VQAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIPTRDEVVL 299 >gi|300310353|ref|YP_003774445.1| ribokinase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 159 bits (402), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 118/306 (38%), Positives = 159/306 (51%), Gaps = 18/306 (5%) Query: 2 IVIIGSVNMDLVLRVPRMPMPGETLAGDKFMTIPGGKGANQAVACARLAAPGTAVAMVAC 61 + ++G DL R P MP G+T+AG F PGGKG+NQAVA AR+ AP V ++ Sbjct: 10 VAVLGIYVADLAFRAPNMPGLGQTIAGTGFAMGPGGKGSNQAVAAARVGAP---VRFISA 66 Query: 62 VGDDAFG----AQMRHSITACGIDDRYIDEVAGEATGIASIMV-DAQAQNSIVIAAGANG 116 +G DAFG + R A + R ID G TG A I V DA N+I++ GA Sbjct: 67 IGKDAFGDFALSLWRRETIAPHV--RVID---GAPTGAAFIYVNDASGDNAIIVVPGAAS 121 Query: 117 RLDVERIERARPLIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNPAPAQALPRELL 176 +L + +ER R IE A + + QLE P + +++A A G T V NPAPA A P ++ Sbjct: 122 QLSEQDVERERQAIEAAKVFVTQLEQPPEAALAGLQIARAAGTTTVFNPAPALAFPAQVY 181 Query: 177 QKIDYLILNEIEAAMLAE---QQSEDIPLLADKLHALGARNVVVTLGEKGVYGSFADGQQ 233 D++ NE EAA+L +D AD L G ++TLG +G + Q Sbjct: 182 GLCDFITPNEHEAALLTGIEIHHIDDARRAADVLLERGVGCALITLGAQGSL-LHSRSQS 240 Query: 234 RHLPARKV-QAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSVTRVGAQTSIP 292 HLPA V+T AGD F G F +A+G EA A A AA+SVTR G S+P Sbjct: 241 LHLPALCAGTVVETAGAGDAFNGAFAAGLAEGMSAEEAARLATAVAAISVTRPGTAPSMP 300 Query: 293 TRDEVV 298 +R E + Sbjct: 301 SRQEAL 306 >gi|300311076|ref|YP_003775168.1| sugar kinase,ribokinase family protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 74/255 (29%), Positives = 113/255 (44%), Gaps = 19/255 (7%) Query: 58 MVACVGDDAFGAQMRHSITACGIDDRYIDEVAGEATGIASIMVDAQAQNSIVIAAGANGR 117 ++ VGDD FG + +D R++ G +TGI + DA + AG+ Sbjct: 49 YLSAVGDDHFGQDLLALWQREEVDVRHVAIAPGGSTGIYFVTHDAAGHHFHYRRAGSAAS 108 Query: 118 ------LDVERIERARPLIEQASIVLLQLEVPMATVIHSIELAHALGKTVVLNP------ 165 L +E I A L + I L E + ++ A A G T L+ Sbjct: 109 AYQAKALPLEAIAAAGAL-HLSGISLAISESACEAGLAAMAHARASGVTTSLDTNLRLRL 167 Query: 166 ---APAQALPRELLQKIDYLILNEIEAAMLAEQQSEDIPLLADKLHALGARNVVVTLGEK 222 A+A E + D + + + ++L + D + D+L + G + V LG++ Sbjct: 168 WPLERARARMEEAFRLCDICLPSWEDVSLLTGLEDRDA--IVDRLLSYGVKLVAFKLGKE 225 Query: 223 GVYGSFADGQQRHLPARKVQAVDTTAAGDTFIGGFIGAIAQGRDQFEAIAYAQAAAALSV 282 G Y + +D Q+R + V ++D T AGD F G F+ + GRD F A YA AAALS Sbjct: 226 GCYVATSD-QRRMVAPYPVDSLDATGAGDCFGGAFMAELMAGRDAFAAARYANVAAALST 284 Query: 283 TRVGAQTSIPTRDEV 297 T GA IP R +V Sbjct: 285 TGYGAVEPIPRRAQV 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 850,086 Number of extensions: 33963 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 4 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)