BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2450.p (493 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 463 e-131 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 313 2e-86 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 299 3e-82 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 291 8e-80 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 282 5e-77 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 463 bits (1192), Expect = e-131, Method: Compositional matrix adjust. Identities = 236/494 (47%), Positives = 317/494 (64%), Gaps = 15/494 (3%) Query: 11 KQYKLLINGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAYKTWKDTS 70 ++Y I G+++P G F + +P G A + EDV+ A+ AA +A K+W TS Sbjct: 17 QRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTS 76 Query: 71 PAERAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIRTDE 130 P ERA L +IAD +EA + A ETLDNGKPIRET+A DIP IDHFRYFAAA+RT E Sbjct: 77 PTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 Query: 131 GSATRLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLS 190 GS +D +T + EP+GVVGQIIPWNFP LMA WKLAPALAAGNCVV+KP+ T S Sbjct: 137 GSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPAS 196 Query: 191 LLELGKVLKDILPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYTVAEAAAKKL 250 ++ L +++ D++P GVVNIV G G G + ++ K+AFTG T G + + A++ L Sbjct: 197 IMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNL 256 Query: 251 IPSTLELGGKSANIIFPDA------PWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYN 304 IP TLELGGKS NI F D ++K +EG + L NQG+VC SR+ V +SIY Sbjct: 257 IPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFAL-NQGEVCTCPSRVLVQESIYE 315 Query: 305 RFVEEAGKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEG-EVLVGGHRIGR 363 RF+E A K + + P +T +G + QL+ +L Y+DIG+ EG +VL GG GR Sbjct: 316 RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG---GR 372 Query: 364 TE----LQDGNFMEPTLIATTNNSRVAREEIFGPVGAVIAFEDEDEVIAMANDSEFGLGG 419 E L G +++PT+ N R+ +EEIFGPV +V F+DE+E +A+AND+ +GLG Sbjct: 373 EELGGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGA 432 Query: 420 AVWTKTLNTALRVASGVETGRMWVNTYNQLPAHAPFGGYKKSGIGRENHKMMLEHYSQCK 479 +WT+ A R+ ++ GR+W N Y+ PAHA FGGYK+SGIGRENHKMML+HY Q K Sbjct: 433 GLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTK 492 Query: 480 NIFISMDETKLGLY 493 N+ +S LG + Sbjct: 493 NLLVSYSPKALGFF 506 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 313 bits (802), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 190/488 (38%), Positives = 270/488 (55%), Gaps = 18/488 (3%) Query: 4 LHTEHIDKQYKLLINGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAY 63 LH +H I GE A G NP + E+ A A DV AV AA + Sbjct: 3 LHLQH-------FIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQL 55 Query: 64 K--TWKDTSPAERAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRY 121 + W S +R L+++A +E A ++ G+ E +D+P+ I H R Sbjct: 56 EGGAWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRA 115 Query: 122 FAAAIRTDEG----SATRLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGN 177 A EG +A + K TLS +REPIGVVG I+PWN P ++ +WK+A LAAG Sbjct: 116 AAGWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGC 175 Query: 178 CVVIKPSSDTSLSLLELGKVLKDI-LPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGST 236 VVIKP+ +T S L L ++ ++ P GV+N+VTG G++ G + EH K++FTGS Sbjct: 176 TVVIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSP 235 Query: 237 DIGYTVAEAAAKKLIPSTLELGGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRI 296 + G + A ++ TLELGGKS I+F DAP+E + G LG+ NQGQVC AGSRI Sbjct: 236 EAGRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRI 295 Query: 297 FVHKSIYNRFVEEAGKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEGEVLV 356 V +S+ RF + +A + + V P +MGP+ Q + V Y+ G EG L+ Sbjct: 296 LVQRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLL 355 Query: 357 GGHRIGRTELQDGNFMEPTLIATTNNS-RVAREEIFGPVGAVIAFEDEDEVIAMANDSEF 415 G G + G F++PT+ A NN +AREEIFGPVG +I+F+ E+E IA+ANDS++ Sbjct: 356 AG---GVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKY 412 Query: 416 GLGGAVWTKTLNTALRVASGVETGRMWVNTYNQLPAHAPFGGYKKSGIGRENHKMMLEHY 475 GL VWT L A RVA+GV+ G + VN ++ L A+ P+GG K SG+GRE Y Sbjct: 413 GLAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAY 472 Query: 476 SQCKNIFI 483 ++ K + + Sbjct: 473 TEEKTVTV 480 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 299 bits (766), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 181/435 (41%), Positives = 255/435 (58%), Gaps = 16/435 (3%) Query: 29 EFTSTNPASGELLAHCANADREDVDAAVQAAWSAYKTWKDTSPAERAGFLNRIADAIEAK 88 + +PASG+LLAH + DVDAAVQAA +A W+ RA L +A ++ Sbjct: 47 DLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQALGGHGRARHLYALARTVQRH 106 Query: 89 AQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIRTDEGSATRLDKNTLSLILREP 148 A+ A++ETLDNGKP+RE+ VD+P HF ++A + E P Sbjct: 107 ARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQLQESEFPD----------HVP 156 Query: 149 IGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLSLLELGKVLKDI-LPKGVV 207 +GVVGQI+PWNFP LM AWK+APALA GN VV+KP+ +TSL+ L ++ + LP GV+ Sbjct: 157 VGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTALLFAELAQQAGLPAGVL 216 Query: 208 NIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYTVAEAAAKKLIPSTLELGGKSANIIFP 267 NIVTG G+T G ++ H G +K+AFTGST++G + E A TLELGGKS I+F Sbjct: 217 NIVTGDGAT-GAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGKSLTLELGGKSPFIVFE 275 Query: 268 DAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYNRFVEEAGKAFSQVNVNLPWLANT 327 DA + IEGV I FNQGQVCCAGSR+ V + I++ F+ ++ V P + Sbjct: 276 DADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPLDKCS 335 Query: 328 QMGPLVNAHQLKTVLGYVDIGRTEGEVLVGGHRIGRTELQDGNFMEPTLIATTN-NSRVA 386 MG L++ QL+ V V+ G EG H++ G F PTL+ + + VA Sbjct: 336 DMGALISPVQLERVRSLVEQGVREGAQC---HQVVLDTPPGGCFYPPTLLTGVHPAATVA 392 Query: 387 REEIFGPVGAVIAFEDEDEVIAMANDSEFGLGGAVWTKTLNTALRVASGVETGRMWVNTY 446 EEIFGPV ++F DE + +AN+S +GL ++W++T+ AL VA ++ G +W+N+ Sbjct: 393 SEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGVAPQLKAGVVWINST 452 Query: 447 NQLPAHAPFGGYKKS 461 NQ A FGG ++S Sbjct: 453 NQFDAAVGFGGVRES 467 Score = 80.1 bits (196), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 73/235 (31%), Positives = 106/235 (45%), Gaps = 6/235 (2%) Query: 9 IDKQYKLLINGEWIPARSGNEFT-STNPASGELLAHCANADREDVDAAVQAAWSAYKTWK 67 ID+ KL I G+ + R E + + + GE L +R+D+ AV AA W Sbjct: 514 IDRTAKLYIGGKQV--RPDGEVSMACHSTQGERLEDVGLGNRKDIRNAVAAA-VKAGGWT 570 Query: 68 DTSPAERAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIR 127 SP RA L IA+ + A+ + FA G E A ++ + I + A Sbjct: 571 SASPHRRAQGLYYIAENLSARTEEFARRIASTTGVTADEARA-EVDASIKRLFTYGAWAD 629 Query: 128 TDEGSATRLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDT 187 EG+ + ++L + E IGVVG + P P L +AP +A GN VV+ PS Sbjct: 630 KFEGAVHQPPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQA 689 Query: 188 SLSLLELGKVLK-DILPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYT 241 L +L +VL LP GV+NIVTGK + + EH+ L G + T Sbjct: 690 PLVATDLYQVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRGPAEFSAT 744 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 291 bits (745), Expect = 8e-80, Method: Compositional matrix adjust. Identities = 184/476 (38%), Positives = 258/476 (54%), Gaps = 9/476 (1%) Query: 11 KQYKLLINGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAYKTWKDTS 70 K+ INGE + A G NPA+G + V+AA AA A + W S Sbjct: 21 KEIGSWINGELV-AGQGEIIQLYNPATGRASLSYRDGGAAAVEAAAVAAQRAQRQWWALS 79 Query: 71 PAERAGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIRTDE 130 A R L + I A+A+ A +E + +GKPIR+ A ++ + F Y+A Sbjct: 80 HAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRA-EMQKVAEMFEYYAGWADKFY 138 Query: 131 GSATRLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLS 190 G + + L+ REP G V Q+ PWN P W+L PALA GN V++KPS T S Sbjct: 139 GEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSELTPFS 198 Query: 191 LLELGKVLKDI-LPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYTVAEAAAKK 249 L + ++ + LP G+VN++ G G T + +K+ F GS G +A+AAA + Sbjct: 199 SLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAAR 258 Query: 250 LIPSTLELGGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYNRFVEE 309 ++P LELGGKSANIIF DA G Q I GQ C AGSR+ V + +Y+RFVE Sbjct: 259 VMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVET 318 Query: 310 AGKAFSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEGEVLVGGH-RIGRTELQD 368 ++ + P +T++GP+ N Q + + V G G L GH R G ++ Sbjct: 319 VAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYG----EE 374 Query: 369 GNFMEPTLIATTNNS-RVAREEIFGPVGAVIAFEDEDEVIAMANDSEFGLGGAVWTKTLN 427 G F+ PTL+A +N+ VAR EIFGPV I FEDE+E IA+ANDSEFGL GAVWT+ + Sbjct: 375 GYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVA 434 Query: 428 TALRVASGVETGRMWVNTYNQLPAHAPFGGYKKSGIGRENHKMMLEHYSQCKNIFI 483 A RVA+ V G WVN+Y + +PFGG+ +SG GR + L Y+Q K++++ Sbjct: 435 RAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWV 490 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 282 bits (721), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 164/472 (34%), Positives = 249/472 (52%), Gaps = 6/472 (1%) Query: 16 LINGEWIPARSGNEFTSTNPASGELLAHCANADREDVDAAVQAAWSAYK-TWKDTSPAER 74 I W+ SG +P+ G+ A + D+D AV+AA AY+ W S AER Sbjct: 5 FIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAER 64 Query: 75 AGFLNRIADAIEAKAQHFAMVETLDNGKPIRETLAVDIPSGIDHFRYFAAAIRTDEGSAT 134 + L ++A ++ + A +E D GKP+++ A D + + +F ++A A G Sbjct: 65 SRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGDTI 123 Query: 135 RLDKNTLSLILREPIGVVGQIIPWNFPFLMAAWKLAPALAAGNCVVIKPSSDTSLSLLEL 194 L LREP GV G I+PWN+P + + ALAAGN V+KP+ D LS+L + Sbjct: 124 PYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILRV 183 Query: 195 GKVLKDI-LPKGVVNIVTGKGSTTGNYMLEHEGFRKLAFTGSTDIGYTVAEAAAKKLIPS 253 ++ ++ P+G +NIVTG G G+ +++H ++FTGS ++G V AAA+ P Sbjct: 184 AELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPV 243 Query: 254 TLELGGKSANIIFPDAPWEKMIEGVQLGILFNQGQVCCAGSRIFVHKSIYNRFVEEAGKA 313 TLELGGKS I+F DA E M+ V I+ N GQ C AGSR+ V + Y + +E Sbjct: 244 TLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGL 303 Query: 314 FSQVNVNLPWLANTQMGPLVNAHQLKTVLGYVDIGRTEGEVLVGGHRIGRTELQDGNFME 373 FSQ+ V + + GPL+ Q + V G++ + +G L +I G + Sbjct: 304 FSQLQVGSSQM-DLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQA 362 Query: 374 PTLIA-TTNNSRVAREEIFGPVGAVIAFEDEDEVIAMANDSEFGLGGAVWTKTLNTALRV 432 P L+ R+A+EE+FGPV A + FEDE IA+AN +++GL +VWT+ +R+ Sbjct: 363 PALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRL 422 Query: 433 ASGVETGRMWVNTYNQLPA-HAPFGGYKKSGIGRENHKMMLEHYSQCKNIFI 483 A + +G++++N Y PFGG K SG GRE L ++ K I I Sbjct: 423 ARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,557,997 Number of extensions: 67602 Number of successful extensions: 286 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 217 Number of HSP's successfully gapped: 21 Length of query: 493 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 404 Effective length of database: 1,198,455 Effective search space: 484175820 Effective search space used: 484175820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)