BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2446.p (485 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310106|ref|YP_003774198.1| class III aminotransferase [Her... 293 2e-80 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 259 4e-70 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 229 4e-61 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 212 7e-56 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 144 1e-35 >gi|300310106|ref|YP_003774198.1| class III aminotransferase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 293 bits (751), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 196/470 (41%), Positives = 264/470 (56%), Gaps = 45/470 (9%) Query: 25 MNHIVGRALEQRDYRAIHLARHLLPFEDHKFLQPEHVR--------VIVRGQGLYVWDDH 76 MNH +G AL + P +DH L +R IVRG G+ VWD H Sbjct: 1 MNHFIGHALAR-------------PGDDHHTLSSAFLRRLSQGAGHAIVRGAGMQVWDAH 47 Query: 77 GRRLLDGMSGLWCTTLGYGRQELVAAATRQMTTLSYCSQFFNTAHPTLAELSARLFELLP 136 LLDG+ GL+ TLG+ +AAA R M+ A P A+L+ Sbjct: 48 DACLLDGVCGLYGNTLGH-GHAALAAAARPMS--------LPAADPLAAQLALNAALSAR 98 Query: 137 AHY--SRILYCNSGSEANEILIKVVRRYWELAGRTSKRVIIGRHNGYHGSTVGSASLGGM 194 +R+L ++ A + +++ VR YW G+ +R+II H+G + V Sbjct: 99 LSAAGARVLSASTHEIAWQAMLRAVRHYWTAVGQPDRRLIITCHDGEPEAAVSGR----- 153 Query: 195 KIMHALGASMLPEVQ-HIEQPFWFGYDGPLNEVEFGLHAAQALERKIIELGADNVAAFVA 253 G PE I P WFG+DG LNE EFGL AA+ LE++I++LG ++VAAFVA Sbjct: 154 ------GRRSAPEDSLRIAAPDWFGHDGYLNEYEFGLAAARTLEQRILDLGPEHVAAFVA 207 Query: 254 EPFQGAGGMIFPPDNYWPEIQRICQRHEVLLCADEVIGGFGRTGHWFAHEYFGFQPDLLT 313 F GA G++ PP +YWPEIQRIC RH VLLC DE IGG GRTG WFAH F PD++ Sbjct: 208 HAFTGASGLLHPPYSYWPEIQRICSRHHVLLCIDEGIGGCGRTGKWFAHTQLRFSPDIVL 267 Query: 314 IAKGLTSGYVPMGGLVLSERVGETLAEC-GGLFAHGLTYQGHPVAAAVALATLSLLDEGG 372 +A+GLTS P+ LVLS + +TLA G L G Q P A A+ALA L LDEGG Sbjct: 268 LAQGLTSALAPLAALVLSPPMADTLAAVEGALPVDGRIDQADPTATAIALANLRALDEGG 327 Query: 373 VLARLATCTGPYLQAQLRSRLGAHVLVNEIQGTGAVAALQLALGKDGKQRFANENAVGLH 432 + ++ TG YLQ LR R H LV +IQG G +AALQL+ + RFA+E A G+ Sbjct: 328 LARQVEHDTGLYLQHCLRERFDNHPLVGDIQGNGMLAALQLSPDPARRARFADEAAAGID 387 Query: 433 CMRRAQHHGLIVRNSGARIILAPALVASLAELDELIDKLTLALDDTARAL 482 C R+A G++VR S ARI+LAP ++A+ A++D L+++L A+D +AR + Sbjct: 388 CARQALAQGVLVRASAARILLAPPMIATHADIDGLVERLGAAVDASARRM 437 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 259 bits (661), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 164/451 (36%), Positives = 235/451 (52%), Gaps = 12/451 (2%) Query: 32 ALEQRDYRAIHLARHLLPFEDHKFLQPEHVRVIVRGQGLYVWDDHGRRLLDGMSGLWCTT 91 AL D R HL + +H+ L P ++ GQG Y+ D ++LLD SGLWC Sbjct: 5 ALAALDRR--HLIHPVTSLREHEELGP---LILKSGQGAYLTDHQDKQLLDAFSGLWCVN 59 Query: 92 LGYGRQELVAAATRQMTTLSYCSQFFNTAHPTLAELSARLFELLPAHYSRILYCNSGSEA 151 GYG++ ++ AAT QM L Y + +F+ A L+ +L ++ PA + + GS+A Sbjct: 60 TGYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDA 119 Query: 152 NEILIKVVRRYWELAGRTSKRVIIGRHNGYHGSTVGSASLGGMKIMHALGASMLPEVQHI 211 + I+ + Y G+ K+ I GYHGS+ A L + H LP ++ Sbjct: 120 VDAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYL 179 Query: 212 EQPFWFGYDGPLNEVEFGLHAAQALERKIIELGADNVAAFVAEPFQGAGGMIFPPDNYWP 271 P+ + + +E + QAL K+ ELGADNVAAF EP QG+GG++ PP + Sbjct: 180 PSPYPYRSELQGDEAIIAA-SVQALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLK 238 Query: 272 EIQRICQRHEVLLCADEVIGGFGRTGHWFAHEYFGFQPDLLTIAKGLTSGYVPMGGLVLS 331 +Q C ++L DEVI GFGRTG FA QPDL+T+AKGLT+GY PMG +++S Sbjct: 239 AMQLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMS 298 Query: 332 ERVGETLAE---CGGLFAHGLTYQGHPVAAAVALATLSLLDEGGVLARLATCTGPYLQAQ 388 +RV +A+ + HG TY HPV+AAVAL L L +EGG+L P Sbjct: 299 DRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDN-GRRLEPVFAGG 357 Query: 389 LRSRLGAHVLVNEIQGTGAVAALQLALGKDGKQRFANENAVGLHCMRRAQHHGLIVRNSG 448 LR+ L AH LV + + G + AL+L K K RFA E + + A HGL+ R G Sbjct: 358 LRALL-AHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFG 416 Query: 449 ARII-LAPALVASLAELDELIDKLTLALDDT 478 I+ APAL + + + +L LD+ Sbjct: 417 DNILGFAPALCYTQEDFALMFARLKAILDEV 447 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 229 bits (584), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 148/444 (33%), Positives = 226/444 (50%), Gaps = 20/444 (4%) Query: 43 LARHLLPFEDHKFLQPEHVRVIVRGQGLYVWDDHGRRLLDGMSGLWCTTLGYGRQELVAA 102 L+ + +PF ++ + R++V +G++ DD G +LDG +GLWC LG+ ++V+A Sbjct: 12 LSAYWMPFTANRNFKAA-PRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSA 70 Query: 103 ATRQMTTLSYCSQFFNTAHPTLAELSARLFELLPAHYSRILYCNSGSEANEILIKVVRRY 162 + + TL Y F HP+ EL+ RL + + Y SGSEA + +K+ Y Sbjct: 71 VQKSVATLDYAPSF-QLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAY 129 Query: 163 WELAGRTSKRVIIGRHNGYHGSTVGSASLGGMKI-MHALGASMLPEVQHIEQPFWFGYDG 221 G ++ +IGR GYHG G S+GG+ ++LP V H+ + Sbjct: 130 HRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNA 189 Query: 222 -PLNEVEFGLHAAQALERKIIELGADNVAAFVAEPFQGAGGMIFPPDNYWPEIQRICQRH 280 E E+G H A LER + A N+AA + EP G+ G++ PP Y ++ IC +H Sbjct: 190 FTKGEPEYGAHLADELERLVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKH 249 Query: 281 EVLLCADEVIGGFGRTGHWFAHEYFGFQPDLLTIAKGLTSGYVPMGGLVLSERVGETLAE 340 +LL DEVI GFGR G FA +YF +PD+ T AKGLT+G VPMG + + + + Sbjct: 250 GILLIFDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMN 309 Query: 341 C--GGLFAHGLTYQGHPVAAAVALATLSLLDEGGVLARLATCTGPYLQAQLRSRLG-AHV 397 G HG TY GHP+A A LA+L + GVL A Y Q + G HV Sbjct: 310 GPDGIELFHGYTYSGHPLACAAGLASLEVFQTEGVLEN-AQAVADYFQEAAHALRGLPHV 368 Query: 398 LVNEIQGTGAVAALQLAL--GKDGKQRFANENAVGLHCMRRAQHHGLIVRNSGARIILAP 455 + +++ G +A ++LA G+ G + + RA + G+++R +G I L+P Sbjct: 369 I--DVRTIGLIAGIELASIPGRVGARAY--------DAFVRAFNDGILIRVTGDIIALSP 418 Query: 456 ALVASLAELDELIDKLTLALDDTA 479 L+ + ++DEL KL L A Sbjct: 419 PLIINKQQIDELFGKLAAILKTLA 442 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 212 bits (539), Expect = 7e-56, Method: Compositional matrix adjust. Identities = 138/422 (32%), Positives = 222/422 (52%), Gaps = 14/422 (3%) Query: 65 VRGQGLYVWDDHGRRLLDGMSGLWCTTLGYGRQELVAAATRQMTTLSYCSQFFNTAHPTL 124 V G+G+ + D G+R +D G + LG+G ++ A +Q+ L+Y F T P Sbjct: 17 VAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPA- 75 Query: 125 AELSARLFELLPAHYSRILYCNSGSEANEILIKVVRRYWELAGRTSKRVIIGRHNGYHGS 184 EL+A L + P + + + + GSEA E +K+ R+Y+ G+ +R II R YHG+ Sbjct: 76 EELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSYHGN 135 Query: 185 TVGSASLGGMKIMHALGASMLPEVQHIEQPFWFGY--DGPLNEVEFGLHAAQALERKIIE 242 T+G+ ++GG + ML E H+ + + DG ++V++ A LE+KI+ Sbjct: 136 TLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGE-SDVQYVQRLADELEQKILS 194 Query: 243 LGADNVAAFVAEPFQGA-GGMIFPPDNYWPEIQRICQRHEVLLCADEVIGGFGRTGHWFA 301 LGAD V AFVAE GA G + P +Y+ +I+ +C ++ VLL DEV+ G GRTG+ FA Sbjct: 195 LGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLFA 254 Query: 302 HEYFGFQPDLLTIAKGLTSGYVPMGGLVLSERVGETLAECGGLFAHGLTYQGHPVAAAVA 361 E G PD++ IAKGL +GY P+G ++ S+ + + + G F HG TY GH A A A Sbjct: 255 CEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYIGHATACAAA 314 Query: 362 LATLSLLDEGGVLARLATCTGPYLQAQLRSRLGAHVLVNEIQGTGAVAALQLALGKDGKQ 421 +A + E +L + G L+++LR G V +I+G G ++L + K Sbjct: 315 VAVQKTIQEERLLENVRQ-RGEQLRSELRQAFGDQAHVGDIRGRGLFVGVELVAERSSKL 373 Query: 422 RFANENAVGLHCMRRAQHHGLIVR--------NSGARIILAPALVASLAELDELIDKLTL 473 + + A GL+V +G I+LAP +AS ++ E++ +L+ Sbjct: 374 PLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQRLSD 433 Query: 474 AL 475 A+ Sbjct: 434 AV 435 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 144 bits (364), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 117/386 (30%), Positives = 189/386 (48%), Gaps = 25/386 (6%) Query: 66 RGQGLYVWDDHGRRLLDGMSGLWCTTLGYGRQELVAAATRQMTTLSYCSQFFNTAHPTLA 125 R +WD GRR +D +G+ G+ +L+ A QM ++ + + + + Sbjct: 32 RAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTA-YQIVPYASYV 90 Query: 126 ELSARLFELLPAHY-SRILYCNSGSEANEILIKVVRRYWELAGRTSKRVIIGRHNGYHGS 184 EL+ R+ L P +Y + + ++G+EA E IK+ R + T + +I G+HG Sbjct: 91 ELAERINRLTPGNYPKKTAFFSTGAEAVENAIKIARAH------TGRPGVIAFAGGFHGR 144 Query: 185 TVGSASLGGMKIMHALGASMLP-EVQHIEQPFWFGYDGPLNEVEFGLHAAQALERKIIEL 243 T+ +L G + LG P +V H P+ G +E L A + L + IE Sbjct: 145 TMMGMALTGKVAPYKLGFGPFPGDVFH--APYPSALHGITSED--ALEAVKGLFKSDIE- 199 Query: 244 GADNVAAFVAEPFQGAGGMIFPPDNYWPEIQRICQRHEVLLCADEVIGGFGRTGHWFAHE 303 A VAA + EP QG GG P ++ ++ +C H +LL ADEV G+GRTG FA E Sbjct: 200 -AKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAME 258 Query: 304 YFGFQPDLLTIAKGLTSGYVPMGGLVLSERVGETLAECGGLFAHGLTYQGHPVAAAVALA 363 ++ PDL+T+AK L G +P+ + + + A GGL G TY G+P+A A ALA Sbjct: 259 HYDVLPDLMTMAKSLAGG-MPLSAVNGRAEIMDAPAP-GGL---GGTYAGNPLAIASALA 313 Query: 364 TLSLLDEGGVLARLATCTGPYLQAQLRSRLGAHVLVNEIQGTGAVAALQLALGKDGKQRF 423 L +++E ++ R G LQ L+ + + E++G GA+ A++ A GK Sbjct: 314 VLDVMEEEQLVTR-GQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDA 372 Query: 424 ANENAVGLHCMRRAQHHGLIVRNSGA 449 V H + ++GL++ G+ Sbjct: 373 EYTKKVQQHAL----NNGLLLLTCGS 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,175 Number of extensions: 70432 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 10 Length of query: 485 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 396 Effective length of database: 1,198,455 Effective search space: 474588180 Effective search space used: 474588180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)