BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2428.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310110|ref|YP_003774202.1| hypothetical protein Hsero_0775... 72 2e-14 >gi|300310110|ref|YP_003774202.1| hypothetical protein Hsero_0775 [Herbaspirillum seropedicae SmR1] Length = 139 Score = 71.6 bits (174), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 54/128 (42%), Positives = 73/128 (57%), Gaps = 11/128 (8%) Query: 15 PTGAEMDSFSSTLKNASSGDLLKALGKDGMEPWQKDAIVKELENRAQQAQKSDSNNGANG 74 P+ +++D F L+ S+ DL+K L WQKDAI++EL+NRA ++ S GA G Sbjct: 21 PSQSQIDGFKQGLQGKSNEDLMKGLMDPNTAQWQKDAIMQELQNRADKSGGSQGAGGAGG 80 Query: 75 ANGGGGGGGGDDDDIKKLLKKLTKGTISPQEEQKLAGALGVDTKTLDAAKGGDAGGDSG- 133 DDDI+KLLKKL G+IS +E +KLAG +G+ + L A KG G G Sbjct: 81 G---------GDDDIQKLLKKLMDGSISDEELKKLAGLMGMKPEDLQAIKGAVQGEGVGE 131 Query: 134 -GGDIKGG 140 GDI GG Sbjct: 132 QQGDISGG 139 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.302 0.127 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 220,555 Number of extensions: 5036 Number of successful extensions: 19 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 18 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 17 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 43 (21.7 bits) S2: 126 (53.1 bits)