BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2425.p (972 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310112|ref|YP_003774204.1| hypothetical protein Hsero_0777... 383 e-107 >gi|300310112|ref|YP_003774204.1| hypothetical protein Hsero_0777 [Herbaspirillum seropedicae SmR1] Length = 579 Score = 383 bits (984), Expect = e-107, Method: Compositional matrix adjust. Identities = 232/527 (44%), Positives = 301/527 (57%), Gaps = 63/527 (11%) Query: 507 DGLLGQLDFAAAVKNGSLDSNTTMPTIALPNNVLPSPYSAGNTMDHFLKDNNIDLLSAND 566 D L LD +KN + N LPS A T++ F KDNNIDLLS+ Sbjct: 54 DSFLDSLDIPDVLKNLLREMMGQASAGGGSGNGLPSESGAAKTINQFQKDNNIDLLSSKQ 113 Query: 567 VNNIAETGYYQDQDGKLKAVPPEVQQAAQALMANGGQLFKELESANTGKQDGLLGQLDFA 626 V +A+TGY+ D+DG K VPPEVQ AA +M NGG+LFK+LESA+ GK DGLL Q+D++ Sbjct: 114 VQQMAQTGYFTDKDGNTKQVPPEVQLAAMKMMDNGGELFKKLESAHNGKHDGLLSQVDYS 173 Query: 627 AAVKNHGLDKQAS--------ALPTDFPTIVANNSVL----PSAYSAGNTMDHFLKDNKI 674 A+++ L LP DF N + P Y A T++ F KDN I Sbjct: 174 DALQDGSLSNTPGQRNTPYQPGLPLDFILQALMNGHVSKDQPDDYDAAKTINQFQKDNNI 233 Query: 675 DLLSANDVKNIAETGYYQDQDGKLKAVPPEVQQAAQALMANGGQLFKELEAANTGKQDGL 734 DLLS+ ++ +A+TGY+ D+DG K VPPEVQ AAQA M NGG+LFK+LE+A GK DG Sbjct: 234 DLLSSEQMQQMAQTGYFTDKDGNTKQVPPEVQAAAQAFMDNGGELFKKLESALNGKHDGK 293 Query: 735 LGQLDFAAAVKNGSLDSNTST--------------------------PTIATPNIVLPSA 768 L Q+DF AVK+G++ P+ LP Sbjct: 294 LSQVDFTEAVKDGTIAPGYGNGNGNGNGNGNFGFGNGNGNGFGFGLDPSQFGGGSNLPGG 353 Query: 769 YSAGNTMDHFLKDNKIDLLSANDVKNIAETGYYQDQDGKLKAVTPEVQQAAQALMANGGQ 828 Y A T++ F KDN IDLLS+ V+ +A+TGY+ D+DG K VTPEVQ AAQ++MANGG+ Sbjct: 354 YDAAKTINQFQKDNNIDLLSSEQVQQMAQTGYFTDKDGNTKQVTPEVQAAAQSMMANGGE 413 Query: 829 LFKELEAANTGKQDGLLGQLDFAAAVKNGSLDSSTTM----------------------- 865 LFK+LE+A GK DG L Q+DF A+K+G++ Sbjct: 414 LFKQLESALNGKHDGKLSQVDFTEAIKDGTIAPGYGNGNGNGNGNGNGNYGVGNGNNNGY 473 Query: 866 -PTIALPNNVLPSASSAGNTMDHFLKDNKIDLLSANDVKNIADTGYYQDQDGKLKAVPPE 924 PT LPS S+A NT++ + KDN I L+SA+ + +A TGY+ D+DG K VPPE Sbjct: 474 DPT-QFGGRTLPSDSTAVNTIEKYQKDNGIGLISADQMLQMAQTGYFTDKDGNTKQVPPE 532 Query: 925 VQQAAQALMANGGQLFKELEAANTGKQDGLLGQLDFAAAVKAGQFSA 971 VQ AAQA MANGG+LFK++EAA TGK DGLLGQ D A + G SA Sbjct: 533 VQAAAQAYMANGGELFKKIEAAVTGKHDGLLGQNDPGQAREDGTVSA 579 Score = 380 bits (975), Expect = e-106, Method: Compositional matrix adjust. Identities = 242/561 (43%), Positives = 323/561 (57%), Gaps = 65/561 (11%) Query: 24 PGALPG-----GQFNPESSDILL------KEGDGSTSNMVNNLPISDNLKGAILSLMGLG 72 PGA PG Q + E DI L ++GD + +++L I D LK + +MG Sbjct: 22 PGAPPGLFNFGNQQSQEYGDIHLFGNSPGQQGD----SFLDSLDIPDVLKNLLREMMGQA 77 Query: 73 GGRT------PSSLSAANTIRNFQQENNTGLLSAKQMSDIADTGYYQDKNGNLKPVSADV 126 PS AA TI FQ++NN LLS+KQ+ +A TGY+ DK+GN K V +V Sbjct: 78 SAGGGSGNGLPSESGAAKTINQFQKDNNIDLLSSKQVQQMAQTGYFTDKDGNTKQVPPEV 137 Query: 127 QAAAQALMANGGALFKQLEAANTGKQDGLLGPKDFPAAMRQGILSGDSGANRAVPGNGLA 186 Q AA +M NGG LFK+LE+A+ GK DGLL D+ A++ G LS G GL Sbjct: 138 QLAAMKMMDNGGELFKKLESAHNGKHDGLLSQVDYSDALQDGSLSNTPGQRNTPYQPGLP 197 Query: 187 GDYLLQAVMQGLLSNGQ-SGYEAGKTIDSFMKDNKIDLLSANDVRDMAKTGYYKDKDGSL 245 D++LQA+M G +S Q Y+A KTI+ F KDN IDLLS+ ++ MA+TGY+ DKDG+ Sbjct: 198 LDFILQALMNGHVSKDQPDDYDAAKTINQFQKDNNIDLLSSEQMQQMAQTGYFTDKDGNT 257 Query: 246 KPIPENVREAAEELMENDGELFKKLEAANTGVQDGLLGQKDFAAAVKNGSL-DGRNGMGS 304 K +P V+ AA+ M+N GELFKKLE+A G DG L Q DF AVK+G++ G Sbjct: 258 KQVPPEVQAAAQAFMDNGGELFKKLESALNGKHDGKLSQVDFTEAVKDGTIAPGYGNGNG 317 Query: 305 ASAWNF-----------------PTSMTSNNVLPSAYSAGNTMDHFLKDNKIDLLSANDV 347 N P+ + LP Y A T++ F KDN IDLLS+ V Sbjct: 318 NGNGNGNFGFGNGNGNGFGFGLDPSQFGGGSNLPGGYDAAKTINQFQKDNNIDLLSSEQV 377 Query: 348 KNIADTGYYQDQDGKLKAVPPEVQQAAQALMANGGQLFKELESANTGKQDGLLGQLDFAA 407 + +A TGY+ D+DG K V PEVQ AAQ++MANGG+LFK+LESA GK DG L Q+DF Sbjct: 378 QQMAQTGYFTDKDGNTKQVTPEVQAAAQSMMANGGELFKQLESALNGKHDGKLSQVDFTE 437 Query: 408 AVKNGSLDSNTTM------------------------PTVALPNIVLPSAYSAGNTMDHF 443 A+K+G++ PT LPS +A NT++ + Sbjct: 438 AIKDGTIAPGYGNGNGNGNGNGNGNYGVGNGNNNGYDPT-QFGGRTLPSDSTAVNTIEKY 496 Query: 444 LKDNNIDLLSANDVKNIAETGYYQDQDGKLKAVPPEVQQAAQALMANGGQLFKELESANT 503 KDN I L+SA+ + +A+TGY+ D+DG K VPPEVQ AAQA MANGG+LFK++E+A T Sbjct: 497 QKDNGIGLISADQMLQMAQTGYFTDKDGNTKQVPPEVQAAAQAYMANGGELFKKIEAAVT 556 Query: 504 GKQDGLLGQLDFAAAVKNGSL 524 GK DGLLGQ D A ++G++ Sbjct: 557 GKHDGLLGQNDPGQAREDGTV 577 Score = 367 bits (943), Expect = e-102, Method: Compositional matrix adjust. Identities = 216/476 (45%), Positives = 284/476 (59%), Gaps = 60/476 (12%) Query: 208 AGKTIDSFMKDNKIDLLSANDVRDMAKTGYYKDKDGSLKPIPENVREAAEELMENDGELF 267 A KTI+ F KDN IDLLS+ V+ MA+TGY+ DKDG+ K +P V+ AA ++M+N GELF Sbjct: 93 AAKTINQFQKDNNIDLLSSKQVQQMAQTGYFTDKDGNTKQVPPEVQLAAMKMMDNGGELF 152 Query: 268 KKLEAANTGVQDGLLGQKDFAAAVKNGSLDGRNGMGSAS-----AWNFPTSMTSNNVL-- 320 KKLE+A+ G DGLL Q D++ A+++GSL G + +F N + Sbjct: 153 KKLESAHNGKHDGLLSQVDYSDALQDGSLSNTPGQRNTPYQPGLPLDFILQALMNGHVSK 212 Query: 321 --PSAYSAGNTMDHFLKDNKIDLLSANDVKNIADTGYYQDQDGKLKAVPPEVQQAAQALM 378 P Y A T++ F KDN IDLLS+ ++ +A TGY+ D+DG K VPPEVQ AAQA M Sbjct: 213 DQPDDYDAAKTINQFQKDNNIDLLSSEQMQQMAQTGYFTDKDGNTKQVPPEVQAAAQAFM 272 Query: 379 ANGGQLFKELESANTGKQDGLLGQLDFAAAVKNGSLDSNTTM------------------ 420 NGG+LFK+LESA GK DG L Q+DF AVK+G++ Sbjct: 273 DNGGELFKKLESALNGKHDGKLSQVDFTEAVKDGTIAPGYGNGNGNGNGNGNFGFGNGNG 332 Query: 421 --------PTVALPNIVLPSAYSAGNTMDHFLKDNNIDLLSANDVKNIAETGYYQDQDGK 472 P+ LP Y A T++ F KDNNIDLLS+ V+ +A+TGY+ D+DG Sbjct: 333 NGFGFGLDPSQFGGGSNLPGGYDAAKTINQFQKDNNIDLLSSEQVQQMAQTGYFTDKDGN 392 Query: 473 LKAVPPEVQQAAQALMANGGQLFKELESANTGKQDGLLGQLDFAAAVKNGSLDSNTTM-- 530 K V PEVQ AAQ++MANGG+LFK+LESA GK DG L Q+DF A+K+G++ Sbjct: 393 TKQVTPEVQAAAQSMMANGGELFKQLESALNGKHDGKLSQVDFTEAIKDGTIAPGYGNGN 452 Query: 531 ----------------------PTIALPNNVLPSPYSAGNTMDHFLKDNNIDLLSANDVN 568 PT LPS +A NT++ + KDN I L+SA+ + Sbjct: 453 GNGNGNGNGNYGVGNGNNNGYDPT-QFGGRTLPSDSTAVNTIEKYQKDNGIGLISADQML 511 Query: 569 NIAETGYYQDQDGKLKAVPPEVQQAAQALMANGGQLFKELESANTGKQDGLLGQLD 624 +A+TGY+ D+DG K VPPEVQ AAQA MANGG+LFK++E+A TGK DGLLGQ D Sbjct: 512 QMAQTGYFTDKDGNTKQVPPEVQAAAQAYMANGGELFKKIEAAVTGKHDGLLGQND 567 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.128 0.352 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,043,039 Number of extensions: 132236 Number of successful extensions: 358 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 9 Length of query: 972 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 878 Effective length of database: 1,174,780 Effective search space: 1031456840 Effective search space used: 1031456840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 135 (56.6 bits)