BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2423.p (528 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 425 e-120 gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 182 5e-47 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 425 bits (1092), Expect = e-120, Method: Compositional matrix adjust. Identities = 241/531 (45%), Positives = 322/531 (60%), Gaps = 19/531 (3%) Query: 1 MQWHEGYVSDIDYVAAFFPEQSPVLLSFACLLNGCEPPSPERHFRYLELGSGQGLTANIL 60 MQW GYV IDY FFPEQSPV LSFACL G EP +R F Y+ELG GQGLT+ +L Sbjct: 1 MQWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVL 60 Query: 61 AAANPQSDFYAVDFNPAHIAASRQLAQAAGLQNIHFLENSFADLAEGVIDLPPLDFITMH 120 AAANPQ FYAVDF P + ++ +A AAG+ N+ LE SF +A G IDLP DF+TM+ Sbjct: 61 AAANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMY 120 Query: 121 GVYSWVNADNRHHIMRLVNRYLKPGGIVYLSYNAMPGWTTTLPFQRLLLEHATLYPGSKP 180 GVYSWV+ NR HI+ + RYLKPGGIVYL+YNA+PGW++ L QR+L E + Sbjct: 121 GVYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQRMLREFGRHDMCDRQ 180 Query: 181 AQIDKARTLVRGLMETRAGYFTSNTSANLQNYLRGAMEEDASRSPYLAHEYMNQGWEPLY 240 AQ+ + LVR L + A F + + L+ LR A+E D YLAHEY+NQGW+ L+ Sbjct: 181 AQLHQGGALVRALSQAGAAAFGAAEATGLRRRLR-ALESD--EPGYLAHEYLNQGWDALF 237 Query: 241 HIDVARDAAQAKLDYVGSADLSWAFPELYLSVEQRDFLQEIQDPGLRETVKDYLKNTCFR 300 IDV +D AQAKLDYVGSA+L++ FPELYLS Q+ L + + G+RE V D + N+ FR Sbjct: 238 SIDVIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSFR 297 Query: 301 KDIYVRGKRPVSAHRQEQWFGRIGLALTVLRETVSLKMGFPLRQYQPDGEIYNAILDALA 360 +DIYVRG R +S H+++ W R GLALT ++L++ + + D Y ++DAL Sbjct: 298 EDIYVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAHTYQPVMDALC 357 Query: 361 QGPKSLAELHQLPSL--QGKSLGELAQFAALLIDTGQAAPYLLQSVVQDRAPAQRLNQAI 418 Q P S AEL LP+L QG+ L E+A A LL + QAAP+ L + Q R AQRLN+ I Sbjct: 358 QRPHSFAELAALPALRKQGRGLREIASMAILLSASRQAAPFFLSAAAQPRQAAQRLNRLI 417 Query: 419 AQLDT---GYRVFASPLLGSGVAAPLFQRLVYDSL----ADPQAGTMSSTDVASRVLRQV 471 A ++ ASPLLG+G+ + L QR+ +D L + P+ + R + Sbjct: 418 AGWSAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPEQWVLPMAQAIHRQGTHL 477 Query: 472 ESQHVPILLHERELVSEQEKWQEVQETVKAILTRRRPFWKQLLMLPEAAAA 522 + P L + +E E+ +AI T+R P W L M P+ A + Sbjct: 478 QRGGAP-------LSTREEIHAELLALAQAICTQRLPVWANLNMAPQQATS 521 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 182 bits (463), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 161/537 (29%), Positives = 255/537 (47%), Gaps = 44/537 (8%) Query: 3 WHEGYVSDIDYVAAFFPEQSPVLLSFACLLNGCEPPSPERHFRYLELGSGQGLTANILAA 62 W++GYV DI Y ++P +P L A +G + P+ + ELG GQGL++NI AA Sbjct: 4 WNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQTA---CELGFGQGLSSNIHAA 60 Query: 63 ANPQSDFYAVDFNPAHIAASRQLAQAAGLQNIHFLENSFADLAEGVIDLPPLDFITMHGV 122 A+ + ++ DFNP A +++LA A+G ++SF+ DLP DFI +HG+ Sbjct: 61 AS-TTRWWGNDFNPRQAAFAQELAAASG-SGAQLEDSSFSRFCRR-DDLPDFDFIALHGI 117 Query: 123 YSWVNADNRHHIMRLVNRYLKPGGIVYLSYNAMPGWTTTLPFQRLLLEHATLYPGSK--- 179 +SWV+ +NR I ++R LKPGG++Y+SYN PGW+ +P + LL++H T G++ Sbjct: 118 WSWVSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQH-TQQSGNEEQG 176 Query: 180 ---PAQIDKARTLVRGLMETRAGYFTSNTSANLQNYLRGAME--EDASRSPYLAHEYMNQ 234 A+ID A L E Y +N ++R ++ + SRS YLAHEY N+ Sbjct: 177 LPTAARIDAALAFAARLFEADPVYAQANP------FMRDRLKVLQQQSRS-YLAHEYFNR 229 Query: 235 GWEPLYHIDVARDAAQAKLDYVGSADLSWAFPELYLSVEQRDFLQEIQDPGLRETVKDYL 294 W D+ R + A L++ A L+ QR L EI DP L+++V+D++ Sbjct: 230 DWHVESFADMHRRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFM 289 Query: 295 KNTCFRKDIYVRGKRPVSAHRQEQWFGRIGLALTVLRETVSLKMGFPLRQYQPDGEIYNA 354 N FR+D +VRG + + ++ Q + L + V L + + + IY Sbjct: 290 VNQQFRRDYWVRGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEP 349 Query: 355 ILDALAQ-GPKSLAELHQLPSLQGKSLGELAQFAALLIDTGQAAPYLLQSVVQDRAPAQR 413 IL AL+ P +L +L + + +L ++ +LI TG AAP + RA +R Sbjct: 350 ILQALSDYQPHTLVDLAASLAHRNLNLAQVLDSVLMLIGTGHAAPLPAEPDAATRAQIER 409 Query: 414 ----LNQAIAQL------------DTGYRVFASPLLGSGVAAPLFQRLVYDSLADPQAGT 457 LN + Q + +SP++G GV ++L LA G Sbjct: 410 RTGDLNAWLLQRAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFL--LARQSCG- 466 Query: 458 MSSTDV-ASRVLRQVESQHVPILLHERELVSEQEKWQEVQETVKAILTRRRPFWKQL 513 D ++V R + +Q +L L E E E+ + R P K L Sbjct: 467 -DDVDAWPAQVWRYIAAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKAL 522 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,720,485 Number of extensions: 74501 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 2 Length of query: 528 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 438 Effective length of database: 1,193,720 Effective search space: 522849360 Effective search space used: 522849360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)