BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2422.p (513 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase... 513 e-146 gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase... 213 3e-56 >gi|300310113|ref|YP_003774205.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 523 Score = 513 bits (1321), Expect = e-146, Method: Compositional matrix adjust. Identities = 280/516 (54%), Positives = 348/516 (67%), Gaps = 6/516 (1%) Query: 1 MDWNEGYVAGIDYPTGFFREQSPVHLSFACLTHGVEPVALDRPFNYLELGAGQGLTASVL 60 M W+ GYV GIDYP GFF EQSPVHLSFACL GVEPV LDR F Y+ELG GQGLT++VL Sbjct: 1 MQWSSGYVHGIDYPVGFFPEQSPVHLSFACLAQGVEPVPLDREFTYMELGCGQGLTSTVL 60 Query: 61 AAANPQGRFHAIDFSPSHVQSATELAQAAELDNLCILEHSFAELAAGLVDLPPMDFITMY 120 AAANPQGRF+A+DF P+ V++A ++A AA +DNL +LE SF ++A G +DLP DF+TMY Sbjct: 61 AAANPQGRFYAVDFLPTQVRTAQDMASAAGIDNLTVLECSFEQMARGEIDLPAFDFVTMY 120 Query: 121 GVYSWVDAGNRKHIVDLIARYLKPGGIVYLNYNAMPGWSPSLPLQRLILERTQTDRHATH 180 GVYSWVD NR HI+D + RYLKPGGIVYLNYNA+PGWS +L LQR++ E + D Sbjct: 121 GVYSWVDRTNRGHILDFLRRYLKPGGIVYLNYNALPGWSSALSLQRMLREFGRHDMCDRQ 180 Query: 181 EQLVQAREMVQRLQ--ALGATFFDGNPNLQYRLDSLREDKGGYLAHEYMSDGWMPLYHAD 238 QL Q +V+ L A L+ RL +L D+ GYLAHEY++ GW L+ D Sbjct: 181 AQLHQGGALVRALSQAGAAAFGAAEATGLRRRLRALESDEPGYLAHEYLNQGWDALFSID 240 Query: 239 VARAMAGAKMDYIGSGELAQAFPRLYLTPQQQELLDTVDDPMLRETVKDYMTNTSFREDI 298 V + +A AK+DY+GS ELA FP LYL+ QQ LL + +RE V D + N+SFREDI Sbjct: 241 VIQDLAQAKLDYVGSAELAYGFPELYLSHAQQSLLAQQPNAGMRELVWDQICNSSFREDI 300 Query: 299 YVRGARRMSATRREHWLRQIGLALTSLPDMLTLELDLSIGKVKADPRYYQPVLDALVRRP 358 YVRGARRMS +R+ WL + GLALT P +TLEL L+IG V+AD YQPV+DAL +RP Sbjct: 301 YVRGARRMSPHQRDAWLERCGLALTEAPSRITLELPLAIGNVRADAHTYQPVMDALCQRP 360 Query: 359 HSLVELAEIPALFP--LSLEDIGQMAALLTASGQACAYFLSAAELPVAPAQRLNAALAEA 416 HS ELA +PAL L +I MA LL+AS QA +FLSAA P AQRLN +A Sbjct: 361 HSFAELAALPALRKQGRGLREIASMAILLSASRQAAPFFLSAAAQPRQAAQRLNRLIAGW 420 Query: 417 SRFKDHYQVLASPLLGNGLAAGLIQRLLFRQLRRPSDRLDLQQCVQQMHDVLQAQQQPLF 476 S D +Q LASPLLGNGL +GL+QR+ F QL + + + Q V M + Q L Sbjct: 421 SAESDCFQALASPLLGNGLPSGLLQRVAFDQLSQAQSQPE--QWVLPMAQAIHRQGTHLQ 478 Query: 477 REGRVLQTQEEVLGELQHLTEAIVTRRVPIWRTLNM 512 R G L T+EE+ EL L +AI T+R+P+W LNM Sbjct: 479 RGGAPLSTREEIHAELLALAQAICTQRLPVWANLNM 514 >gi|300309852|ref|YP_003773944.1| SAM-dependent methyltransferase [Herbaspirillum seropedicae SmR1] Length = 525 Score = 213 bits (543), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 166/538 (30%), Positives = 258/538 (47%), Gaps = 41/538 (7%) Query: 2 DWNEGYVAGIDYPTGFFREQSPVHLSFACLTHGVEPVALDRPFNYLELGAGQGLTASVLA 61 +WN+GYV I Y G++ +P L A HG++ A+ ELG GQGL++++ A Sbjct: 3 NWNDGYVVDIAYTYGYYPGMNPARLPLAFAFHGLQAPAVQ---TACELGFGQGLSSNIHA 59 Query: 62 AANPQGRFHAIDFSPSHVQSATELAQAAELDNLCILEHSFAELAAGLVDLPPMDFITMYG 121 AA+ R+ DF+P Q+A AA + LE S DLP DFI ++G Sbjct: 60 AASTT-RWWGNDFNPR--QAAFAQELAAASGSGAQLEDSSFSRFCRRDDLPDFDFIALHG 116 Query: 122 VYSWVDAGNRKHIVDLIARYLKPGGIVYLNYNAMPGWSPSLPLQRLILERTQTDRHATHE 181 ++SWV NR I D I R LKPGG++Y++YN PGWS +PL+ L+++ TQ + Sbjct: 117 IWSWVSQENRDIIFDFIDRKLKPGGVLYISYNTQPGWSAFMPLRELLVQHTQQSGNEEQG 176 Query: 182 QLVQAR-----EMVQRLQALGATFFDGNPNLQYRLDSLREDKGGYLAHEYMSDGWMPLYH 236 AR RL + NP ++ RL L++ YLAHEY + W Sbjct: 177 LPTAARIDAALAFAARLFEADPVYAQANPFMRDRLKVLQQQSRSYLAHEYFNRDWHVESF 236 Query: 237 ADVARAMAGAKMDYIGSGELAQAFPRLYLTPQQQELLDTVDDPMLRETVKDYMTNTSFRE 296 AD+ R +GA +++ LTP Q++ L + DP+L+++V+D+M N FR Sbjct: 237 ADMHRRFSGAGLEFACPAHYVDHLDMANLTPAQRQQLYEITDPVLQQSVRDFMVNQQFRR 296 Query: 297 DIYVRGARRMSATRREHWLRQIGLALTSLPDMLTLELDLSIGKVKADPRYYQPVLDALVR 356 D +VRGA+ + +R L + L + PD + L + ++ + Y+P+L AL Sbjct: 297 DYWVRGAQALDDRQRVQALALQSVILLTEPDKVPLTIQNVASEITLNRLIYEPILQALSD 356 Query: 357 -RPHSLVELAEIPALFPLSLEDIGQMAALLTASGQACAYFLSAAELPVAP--AQR----- 408 +PH+LV+LA A L+L + +L +G AA LP P A R Sbjct: 357 YQPHTLVDLAASLAHRNLNLAQVLDSVLMLIGTGH-------AAPLPAEPDAATRAQIER 409 Query: 409 ----LNAALAEASRF---------KDHYQVLASPLLGNGLAAGLIQRLLFRQLRRPSDRL 455 LNA L + + + L+SP++G G+ +++L L R S Sbjct: 410 RTGDLNAWLLQRAALASPAGEQGEEGEITHLSSPVIGGGVRVDRVEQLFL--LARQSCGD 467 Query: 456 DLQQCVQQMHDVLQAQQQPLFREGRVLQTQEEVLGELQHLTEAIVTRRVPIWRTLNML 513 D+ Q+ + AQ + L REG L+ + E L EL R+P+ + L ++ Sbjct: 468 DVDAWPAQVWRYIAAQGKRLLREGVRLEGEAENLAELATQARLFARTRLPLLKALGVV 525 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,677,233 Number of extensions: 74364 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 242 Number of HSP's successfully gapped: 2 Length of query: 513 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 424 Effective length of database: 1,198,455 Effective search space: 508144920 Effective search space used: 508144920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)