BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2405.p (87 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310130|ref|YP_003774222.1| type III secretion HrcS protein... 156 2e-40 >gi|300310130|ref|YP_003774222.1| type III secretion HrcS protein [Herbaspirillum seropedicae SmR1] Length = 87 Score = 156 bits (395), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 76/87 (87%), Positives = 84/87 (96%) Query: 1 MADTLNFFQQGLWMAVMMSAPPLVIATLCGVVVSLIQAVTQIQDQTLPYVVKLMAVAVTL 60 MADT+NFFQQ LWMA+MMSAPPL+IATLCG+ VSLIQAVTQIQDQTLPYVVKL+AVAVTL Sbjct: 1 MADTINFFQQSLWMAIMMSAPPLLIATLCGLGVSLIQAVTQIQDQTLPYVVKLVAVAVTL 60 Query: 61 ATMGRWIGVELLRLADMAMTMVPDIGR 87 A+MGRWIGVE+LRLAD+A TMVPDIGR Sbjct: 61 ASMGRWIGVEMLRLADLAFTMVPDIGR 87 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 225,980 Number of extensions: 5919 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 2 Length of query: 87 Length of database: 1,619,870 Length adjustment: 57 Effective length of query: 30 Effective length of database: 1,349,975 Effective search space: 40499250 Effective search space used: 40499250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 123 (52.0 bits)