BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2402.p (133 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310134|ref|YP_003774226.1| type III secretion HrpB protein... 141 2e-35 >gi|300310134|ref|YP_003774226.1| type III secretion HrpB protein [Herbaspirillum seropedicae SmR1] Length = 134 Score = 141 bits (356), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 69/115 (60%), Positives = 91/115 (79%), Gaps = 1/115 (0%) Query: 19 KNNNVTLDLQPANAADSGEFRAALERHLKNDPVHLKTDNASLGSVIVGRTTHLAAEIKQD 78 ++ + +++QP A++ F++ E++L D + LK + SLGS + RT+ LAAE+KQD Sbjct: 21 RDAGIHMEIQPG-GAEAEHFKSMFEQYLNKDALRLKPNTHSLGSALAQRTSSLAAEVKQD 79 Query: 79 QQYVSKLLEQATRTGDSMQLMKAMMALNDFQLRVQTVSKVVSKAVTSVDSLTKLQ 133 Q YVS+LLEQATRTGDSMQLMKAMMAL+D+Q+RVQT+SK VSKA TSVDSLTKLQ Sbjct: 80 QLYVSRLLEQATRTGDSMQLMKAMMALSDYQIRVQTISKCVSKASTSVDSLTKLQ 134 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.123 0.315 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 293,334 Number of extensions: 10160 Number of successful extensions: 24 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 24 Number of HSP's successfully gapped: 1 Length of query: 133 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 58 Effective length of database: 1,264,745 Effective search space: 73355210 Effective search space used: 73355210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 125 (52.8 bits)