BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2399.p (202 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310137|ref|YP_003774229.1| type III secretion HrpE protein... 146 9e-37 >gi|300310137|ref|YP_003774229.1| type III secretion HrpE protein [Herbaspirillum seropedicae SmR1] Length = 233 Score = 146 bits (369), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 89/188 (47%), Positives = 123/188 (65%), Gaps = 3/188 (1%) Query: 2 SDFAVQRVTLPDHLRPANGVLRLTGLTVTSDAEQLAAQMLAQAREEAAQVRKQAEEEAGH 61 S F + LP LRP +GV+ VT DA AAQ++ QA+ EAA +R+QA +A Sbjct: 6 SSFLAAELCLPPSLRPRHGVVSSVDFRVTEDARLAAAQLVQQAQAEAAGIREQARADALA 65 Query: 62 VVMRQQQEVAQQGAVLLEGLRQAQDDMLERIEEVVVDLAQEVMERLLLELTPRERITAML 121 + +++ +A + LL LR+ + MLE + + VDLA + +RLL++ T RER+ A Sbjct: 66 ALHDEERRIAHEAGQLLARLREREASMLEGVAGLAVDLAHSIFDRLLVDTTARERVMAAC 125 Query: 122 RRIRQEAPPKLHEAVLWVHPDDQPLLP---ASSWEMQTDSALAPGSCRLEAASGEWRSDF 178 RR+R+EAPPKL EAV W+HP+D L A WE++TD+ LA GSCRLEAASGEWR+DF Sbjct: 126 RRVREEAPPKLTEAVAWLHPEDAASLAQEDALPWELRTDARLAQGSCRLEAASGEWRADF 185 Query: 179 ALAVQALR 186 +LA +ALR Sbjct: 186 SLAAEALR 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 550,411 Number of extensions: 18268 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 1 Length of query: 202 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 121 Effective length of database: 1,236,335 Effective search space: 149596535 Effective search space used: 149596535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)