BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2388.p (165 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310148|ref|YP_003774240.1| hypothetical protein Hsero_0813... 172 2e-44 >gi|300310148|ref|YP_003774240.1| hypothetical protein Hsero_0813 [Herbaspirillum seropedicae SmR1] Length = 165 Score = 172 bits (435), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 85/165 (51%), Positives = 113/165 (68%) Query: 1 MWGLIVALVMIAAGEFYSSTSSQALAVVQSNSGREVAAEMALYRQAVIDYFSAHDLRGTS 60 MW +V VMIAA Y++ + QA+ AAE+ +YR AVIDYFS HD+ TS Sbjct: 1 MWNAVVLAVMIAAAGVYTTRNLQAVDQAHQELASADAAEVGIYRNAVIDYFSDHDVVNTS 60 Query: 61 VSIEALKSAKALPSWSVLYQQSDRLLWDNYRDQDGIIYIYSTSLPSTNITADLNQISGNS 120 VS +LKS LPSWS LYQQS L W NYRD DG+IY+Y+TSLP N+ A+L +++ NS Sbjct: 61 VSTASLKSGAYLPSWSRLYQQSGALSWTNYRDADGVIYVYATSLPPRNLAAELARLARNS 120 Query: 121 LLVGVYRRGKSTLQSGLFGDTGIPVAALAGRGIADGAPLWIAMTQ 165 +L+GVY +TLQS ++G+T IPV AL+ I +GAP+W+AMT+ Sbjct: 121 VLLGVYDSAHATLQSPVYGETHIPVTALSSLSIPNGAPVWLAMTK 165 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 508,270 Number of extensions: 19423 Number of successful extensions: 63 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 63 Number of HSP's successfully gapped: 1 Length of query: 165 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 87 Effective length of database: 1,250,540 Effective search space: 108796980 Effective search space used: 108796980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)