BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2380.p (536 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 457 e-129 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 422 e-119 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 419 e-118 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 415 e-117 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 356 2e-99 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 457 bits (1175), Expect = e-129, Method: Compositional matrix adjust. Identities = 257/509 (50%), Positives = 363/509 (71%), Gaps = 3/509 (0%) Query: 4 HLKIAKRLGLGLGLVLVLSCMTTGVALWNLQTLSDASSAMMKKPLMKERLSSDWYRAIDS 63 ++ I +RL LG L+L S + G+ LW L ++ A+ MM +PL ERL +DWY + + Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVA 65 Query: 64 GIMRTTAIAKSSDPSLTAFLATE---GPKKSGDLQEHIGSLLDSDEERALFDKIGVQRKR 120 GI RT AIAKSSDP+L + A E K SG+ Q+ + +LL + EE+ALF KIG RK Sbjct: 66 GIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGELRKL 125 Query: 121 YLAARDQVNQLKAAGETAQALKLLEEQYVPVAKGFQQLMQEFLNVQRHQIDSINLQIERT 180 YL++RD++N+ KAAG T +A+++L++ +VP A +Q M++ ++ QR ++D +I+ Sbjct: 126 YLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAREIDAI 185 Query: 181 AGQSRRLLLILEGLSVLLAAIVVHLLARSIIRPLTEAVSVAQRVARGDLTAELQVGARDE 240 A +SR L+L+LEGL +LL + L I +PL AV V++ VA GDL++E+ V +RDE Sbjct: 186 AAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAVTSRDE 245 Query: 241 VGQLMQSLRSMTLNLRELIGKLRRSIHGIAIASAEIATGNQDLSVRTEQQASSVEETAAA 300 GQL+QSL+ M +LR ++ +R I+ AS+EIA GN DLS RTEQQASS+ ETA+A Sbjct: 246 TGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAETASA 305 Query: 301 MEQIATTVKRNAEHARRADETTAVASSAASAGGDAVARMIQTMETINRSSQKISSIIGVI 360 ME++ +TV++NA++AR+A + AS+ A GG V++++ TM IN SS+KI II VI Sbjct: 306 MEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVDIISVI 365 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELITDSVDKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIKELI DSV KV Sbjct: 366 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELINDSVGKV 425 Query: 421 NAGTALVQEAGKTIHGIVESVESVAAIIGDISSSGQDQANGISEVSHAINLIDDVTQKNA 480 + G+ LV++AG T+ +V SV V I+ +IS++ +Q +GI +++ AI +D VTQ+NA Sbjct: 426 DDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQVTQQNA 485 Query: 481 ALVEQAMAAACAMKDEARNLADLISIFKL 509 ALVE+A AAA +M+ +A L ++S+F+L Sbjct: 486 ALVEEAAAAAASMQTQAGRLMQMVSVFRL 514 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 422 bits (1085), Expect = e-119, Method: Compositional matrix adjust. Identities = 270/536 (50%), Positives = 370/536 (69%), Gaps = 17/536 (3%) Query: 2 FTHLKIAKRLGLGLGLVLVLSCMTTGVALWNLQTLSDASSAMMKKPLMKERLSSDWYRAI 61 F ++KI +RL LG ++L + + +++W LQ ++ A+ MM +PL KERL SDWYR I Sbjct: 4 FANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTI 63 Query: 62 DSGIMRTTAIAKSSDPSLTAFLATEG---PKKSGDLQEHIGSLLDSDEERALFDKIGVQR 118 + + RTTAIAKSSDPSL AF A + + S + Q+ I +L++SD+E+A+F K+G R Sbjct: 64 HTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEVR 123 Query: 119 KRYLAARDQVNQLKAAGETAQALKLLEEQYVPVAKGFQQLMQEFLNVQRHQIDSINLQIE 178 K Y+ RD +++ KA G T +A ++L + KG+ L+Q+ L +QR ID + I+ Sbjct: 124 KDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHIQ 183 Query: 179 RTAGQSRRLLLILEGLSVLLAAIVVHLLARSIIRPLTEAVSVAQRVARGDLTAEL--QVG 236 +SR L+L L L V+L + LA I RPL +AV VA+ VA GDLT + ++ Sbjct: 184 TVYRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDERLA 243 Query: 237 AR-DEVGQLMQSLRSMTLNLRELIGKLRRSIHGIAIASAEIATGNQDLSVRTEQQASSVE 295 AR DE G+LM +L +MT NL ++G++R IA AS EIATGN DLS RTE+QA ++E Sbjct: 244 ARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAGALE 303 Query: 296 ETAAAMEQIATTVKRNAEHARRADETTAVASSAASAGGDAVARMIQTMETINRSSQKISS 355 ETA+AME++ +TV +NA++AR+A++ A ASS A GG+ V+++I TM +IN SS+KI Sbjct: 304 ETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRKIVD 363 Query: 356 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELITD 415 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAKEIK LI D Sbjct: 364 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKALIDD 423 Query: 416 SVDKVNAGTALVQEAGKTIHGIVESVESVAAIIGDISSSGQDQANGISEVSHAINLIDDV 475 SV KV +G+ LV AG T+ +V SV VA I+ +IS++ +Q++GIS+V+ AI +D Sbjct: 424 SVAKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDMDQT 483 Query: 476 TQKNAALVEQAMAAACAMKDEARNLADLISIFKLEG------APE-----TPALTA 520 TQ+NAALVE+A AAA AM+++A L++++S FKL G AP TP L A Sbjct: 484 TQQNAALVEEAAAAAEAMQEQAARLSEVVSQFKLLGQDQIAYAPRSAVDITPPLPA 539 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 249/515 (48%), Positives = 350/515 (67%), Gaps = 3/515 (0%) Query: 1 MFTHLKIAKRLGLGLGLVLVLSCMTTGVALWNLQTLSDASSAMMKKPLMKERLSSDWYRA 60 M ++I RLG G +VLV S + V +W L+T++ + MM PL ERL S W Sbjct: 1 MIKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTL 60 Query: 61 IDSGIMRTTAIAKSSDPSLTAFLATEG---PKKSGDLQEHIGSLLDSDEERALFDKIGVQ 117 + I RTT + KS DP L AFLA E K+S + + LL S+EE+ +F I V Sbjct: 61 LLIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVA 120 Query: 118 RKRYLAARDQVNQLKAAGETAQALKLLEEQYVPVAKGFQQLMQEFLNVQRHQIDSINLQI 177 RK++L RDQ+ K G+ AQ +L ++QYV +AK Q M+ L+ +R +ID I+ QI Sbjct: 121 RKQFLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQI 180 Query: 178 ERTAGQSRRLLLILEGLSVLLAAIVVHLLARSIIRPLTEAVSVAQRVARGDLTAELQVGA 237 ++ A S+ +++LE + +L LL RSI RP+ A++++QRVA GDLT+ Q Sbjct: 181 QQDASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSRAQAMG 240 Query: 238 RDEVGQLMQSLRSMTLNLRELIGKLRRSIHGIAIASAEIATGNQDLSVRTEQQASSVEET 297 RDE+GQL+ SL+ M+ L ++ +R IA+AS EIA GN DLS RTE+QA ++E+T Sbjct: 241 RDELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGALEQT 300 Query: 298 AAAMEQIATTVKRNAEHARRADETTAVASSAASAGGDAVARMIQTMETINRSSQKISSII 357 AA++E++ ++V+ N+++A +A+E + A+S A GG + ++I TM TIN SS+KI II Sbjct: 301 AASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRKIVDII 360 Query: 358 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELITDSV 417 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSAAAAKEIK+LI DSV Sbjct: 361 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRALAQRSAAAAKEIKQLIDDSV 420 Query: 418 DKVNAGTALVQEAGKTIHGIVESVESVAAIIGDISSSGQDQANGISEVSHAINLIDDVTQ 477 KV+AG+ LV++AGKT+ +V SV+ ++G+IS++ + QA GI +V+ A+ +D VTQ Sbjct: 421 GKVSAGSTLVEQAGKTMQEVVGSVQGAHTLVGEISTASRQQAEGIHQVNQAVGQMDGVTQ 480 Query: 478 KNAALVEQAMAAACAMKDEARNLADLISIFKLEGA 512 +NAALVEQA AAA +++D+A L ++ F L+ A Sbjct: 481 QNAALVEQAAAAAKSLEDQAAQLKRAVAFFSLQAA 515 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 415 bits (1066), Expect = e-117, Method: Compositional matrix adjust. Identities = 243/528 (46%), Positives = 350/528 (66%), Gaps = 4/528 (0%) Query: 4 HLKIAKRLGLGLGLVLVLSCMTTGVALWNLQTLSDASSAMMKKPLMKERLSSDWYRAIDS 63 +LKI RL +G +L+L + + +W L ++ DA+ M + L KER + W+ AI Sbjct: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 Query: 64 GIMRTTAIAKSSDPSLTAFLATEGPKKSG---DLQEHIGSLLDSDEERALFDKIGVQRKR 120 +RT A+ KS D + A+ + +S LQ+ + + + EE+ LFD++G R Sbjct: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAA 122 Query: 121 YLAARDQVNQLKAAGETAQALKLLEEQYVPVAKGFQQLMQEFLNVQRHQIDSINLQIERT 180 Y A R + +K AG+ A+A ++ + V + + + + + Q+ ID I R Sbjct: 123 YRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRD 182 Query: 181 AGQSRRLLLILEGLSVLLAAIVVHLLARSIIRPLTEAVSVAQRVARGDLTAELQVGARDE 240 R +LL L + ++L A + LL RSI RPL +AV++A+ VA GDLT+ + +RDE Sbjct: 183 YRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDE 242 Query: 241 VGQLMQSLRSMTLNLRELIGKLRRSIHGIAIASAEIATGNQDLSVRTEQQASSVEETAAA 300 G+L+ +L++M NL +++G++R I AS EIATGN DLS RTE QA S+EETA+A Sbjct: 243 TGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETASA 302 Query: 301 MEQIATTVKRNAEHARRADETTAVASSAASAGGDAVARMIQTMETINRSSQKISSIIGVI 360 MEQ+ +TVK+NA++AR+A+E A AS A+ GG V +++ TM I+ SSQKI+ IIGVI Sbjct: 303 MEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVI 362 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELITDSVDKV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAKEIK+LI DSV+KV Sbjct: 363 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKV 422 Query: 421 NAGTALVQEAGKTIHGIVESVESVAAIIGDISSSGQDQANGISEVSHAINLIDDVTQKNA 480 + G+ LV AG T+ +V+SV V A++ +I+ + Q+Q++GI +V+ AI ID+ TQ+NA Sbjct: 423 DDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNA 482 Query: 481 ALVEQAMAAACAMKDEARNLADLISIFKLEGAPETPALTAT-GSHPTT 527 ALVEQA AAA +M+D+A +L D +S+F+LE + A+ G+ P+T Sbjct: 483 ALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPST 530 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 356 bits (914), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 236/549 (42%), Positives = 351/549 (63%), Gaps = 43/549 (7%) Query: 2 FTHLKIAKRLGLGLG-LVLVLSCMTTGVALWNLQTLSDASSAMMKKPLMKERLSSDWYRA 60 F +L IA RL + L+LV++ G+ L L+D + A+ + + +D Y+ Sbjct: 3 FNNLSIATRLAIAFSILILVIA----GIVALGLARLADINKAL-------DLVVNDRYKK 51 Query: 61 ID-----SGIMRTTAIAKS-----SDPSLTA-------FLATEGPKKSGDLQEHIGSLLD 103 I SG + AI+ +DP+ A L+ E K L + + L+ Sbjct: 52 IAIINTISGKVDDVAISLRNQLLMTDPAQIAAEQKTIESLSAENTK----LYDELTRLVA 107 Query: 104 SDEERALFDKIGVQRKRYLAARDQVNQLKAAGETAQALKLLEEQYVPVAKGFQQLMQEFL 163 + E +A DK+ RK Y A R Q+++L+++G+ A+KL+ + VP+ K + + E Sbjct: 108 NPEAKAKLDKVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELS 167 Query: 164 NVQRHQIDSINLQIERTAGQSRRLLLILEG-LSVLLAAIVVHLLARSIIRPLTEAVSVAQ 222 + Q+ +D Q E A ++ R+++++ G L+ AA + ++RSI RPL+ AV VA+ Sbjct: 168 DTQQKLMDQSVDQAE-AAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAE 226 Query: 223 RVARGDLTAELQVGARDEVGQLMQSLRSMTLNLRELIGKLRRSIHGIAIASAEIATGNQD 282 VA GDLTA + + DE G+L+Q+L +M L+ ++ ++RR + AS +IATGN D Sbjct: 227 TVAAGDLTAVIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLD 286 Query: 283 LSVRTEQQASSVEETAAAMEQIATTVKRNAEHARRADETTAVASSAASAGGDAVARMIQT 342 LS RTE+QAS++EETA+++E++ ++VK+NA+H R++ E A AS A+ GG AV +++QT Sbjct: 287 LSSRTEEQASALEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQT 346 Query: 343 METINRSSQKISSIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRS 402 M IN SS KI II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR LAQRS Sbjct: 347 MGAINDSSHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRS 406 Query: 403 AAAAKEIKELITDSVDKVNAGTALVQEAGKTIHGIVESVESVAAIIGDISSSGQDQANGI 462 A AAKEIKELI DSV++V +GT LV +AG T+ +V S+E V+ I+G+IS++ ++Q++GI Sbjct: 407 ATAAKEIKELINDSVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGI 466 Query: 463 SEVSHAINLIDDVTQKNAALVEQAMAAACAMKDEARNLADLISIFKLEGAPETPALTATG 522 +V+ AI +D+ TQ+NAALVE+A AAA A++D+A+ L DL+ +F+L+ G Sbjct: 467 EQVNQAIMQMDNTTQQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLD--------EVGG 518 Query: 523 SHPTTHALA 531 S P A+A Sbjct: 519 SGPAPSAVA 527 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,144,620 Number of extensions: 36565 Number of successful extensions: 581 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 297 Number of HSP's successfully gapped: 71 Length of query: 536 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 446 Effective length of database: 1,193,720 Effective search space: 532399120 Effective search space used: 532399120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)