BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2354.p (124 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312172|ref|YP_003776264.1| hypothetical protein Hsero_2864... 258 1e-70 >gi|300312172|ref|YP_003776264.1| hypothetical protein Hsero_2864 [Herbaspirillum seropedicae SmR1] Length = 124 Score = 258 bits (658), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 123/124 (99%), Positives = 124/124 (100%) Query: 1 MNQIIAQANGMSTAGGIRSWQPNELDLRRIERMLQKRARYLYVVPRVEMTASGYLIQSPC 60 MNQIIAQANGMSTAGGIRSWQPNELDLRRIERMLQKRARYLYVVPRVEMTASGYLIQSPC Sbjct: 1 MNQIIAQANGMSTAGGIRSWQPNELDLRRIERMLQKRARYLYVVPRVEMTASGYLIQSPC 60 Query: 61 CSRNIDAEGGMVDIALLEYDAGLGVWKLFRKDHTQGGWEFFMLERSLQVLMDCINRDTAR 120 CSRNIDAEGGMVDIALLEYDAGLGVWKLFRKDHT+GGWEFFMLERSLQVLMDCINRDTAR Sbjct: 61 CSRNIDAEGGMVDIALLEYDAGLGVWKLFRKDHTEGGWEFFMLERSLQVLMDCINRDTAR 120 Query: 121 LFWQ 124 LFWQ Sbjct: 121 LFWQ 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 381,245 Number of extensions: 14031 Number of successful extensions: 49 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 49 Number of HSP's successfully gapped: 1 Length of query: 124 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 50 Effective length of database: 1,269,480 Effective search space: 63474000 Effective search space used: 63474000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)