BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2353.p (379 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 678 0.0 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 259 2e-70 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 249 4e-67 gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 238 5e-64 gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 99 5e-22 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/379 (95%), Positives = 368/379 (97%) Query: 1 MDTIIYLDNNATTRPTPEVLAAMQACQHEAWGNPSSKHRVGDAARQRMNSARAEVAALLA 60 MDT IYLDNNATTRPTPEVLAAMQACQ EAWGNPSSKHRVGDAARQRMNSARAEVAALLA Sbjct: 1 MDTTIYLDNNATTRPTPEVLAAMQACQDEAWGNPSSKHRVGDAARQRMNSARAEVAALLA 60 Query: 61 ASAAELVFTSGATEANHMAILGALALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWI 120 ASAAELVFTSGATEANHMAILGALALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWI Sbjct: 61 ASAAELVFTSGATEANHMAILGALALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWI 120 Query: 121 GVDEHGQLQLDELAAAITSDTALVSLMWANNETGVVFPIVQAAELARQRGVLFHTDAVQA 180 VDEHGQLQLDELAAAIT DTALVSLMWANNETGVVFPI QAAELARQRGVLFHTDAVQA Sbjct: 121 AVDEHGQLQLDELAAAITPDTALVSLMWANNETGVVFPIAQAAELARQRGVLFHTDAVQA 180 Query: 181 MGRVPCDVAGMGVDLLSLSAHKLHGPKGIGALYVRKGIKLPPLLFGHQERGRRGGTEDVA 240 +GRVPCDVAG+GVDLLSLSAHKLHGPKGIGALYVRKGIKLPPLLFGHQERGRRGGTEDVA Sbjct: 181 VGRVPCDVAGLGVDLLSLSAHKLHGPKGIGALYVRKGIKLPPLLFGHQERGRRGGTEDVA 240 Query: 241 GIVGLGVAADQARAALPRMLAVSALRQRFERGLACLPQVQINGAGAARLPNTVSVRFGQV 300 GIVGLGVAA QARAALPRM AVSALRQRFERGLA LPQV+INGAGAARLPNTVSVRFGQV Sbjct: 241 GIVGLGVAAAQARAALPRMQAVSALRQRFERGLARLPQVKINGAGAARLPNTVSVRFGQV 300 Query: 301 HAERVLDRLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAAATIRFSLSRDTREDEI 360 HAE VL+RLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQA ATIRFSLSRDTREDEI Sbjct: 301 HAELVLERLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAVATIRFSLSRDTREDEI 360 Query: 361 DAVLAALSEIVQPLQARAA 379 DAVLAAL +IVQPLQARAA Sbjct: 361 DAVLAALLQIVQPLQARAA 379 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 259 bits (663), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 150/377 (39%), Positives = 224/377 (59%), Gaps = 8/377 (2%) Query: 5 IYLDNNATTRPTPEVLAAMQACQHEAWGNPSSK-HRVGDAARQRMNSARAEVAALLAASA 63 IY+D +ATT P V M E +GNP+S+ H G A + + AR EVA L+ A + Sbjct: 20 IYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEVAKLVNADS 79 Query: 64 AELVFTSGATEANHMAILGALALQPQR-RHIVSSLVEHPSTLLLLRHLQAQGVRISWIGV 122 E+++TSGATE+N++AI GA + +HI++ EH + L R L+ G +++ Sbjct: 80 REIIWTSGATESNNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTFRELERIGFEATYLQP 139 Query: 123 DEHGQLQLDELAAAITSDTALVSLMWANNETGVVFPIVQAAELARQRGVLFHTDAVQAMG 182 ++G + ++++AAA+ DT LVS+M NNE GV+ P+ + E R +G++FH+DA QA G Sbjct: 140 QDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGIIFHSDAAQATG 199 Query: 183 RVPCDVAGMGVDLLSLSAHKLHGPKGIGALYVRKGIKL---PPLLFGHQERGRRGGTEDV 239 +V D+ VDL+S SAHK +GPKGIGALYVR+ ++ + G ERG R GT Sbjct: 200 KVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGHERGLRSGTLAT 259 Query: 240 AGIVGLGVAADQARAALPRMLA-VSALRQRFERGLACLPQVQINGAGAARLPNTVSVRFG 298 IVG+G A AR + LA + ALR R +GL + + ING R+P+ ++V F Sbjct: 260 HQIVGMGEAFRLAREEMDSELARIRALRDRLAKGLQEIEETYINGDMEHRVPHNLNVSFN 319 Query: 299 QVHAERVLDRLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAAATIRFSLSRDTRED 358 V E ++ + I SSGSACT++ PS+VL A+G S E A ++IRF++ R T E+ Sbjct: 320 YVEGESLIMAIK--DIAVSSGSACTSASLEPSYVLRALGRSDELAHSSIRFTIGRFTTEE 377 Query: 359 EIDAVLAALSEIVQPLQ 375 +ID +A + V L+ Sbjct: 378 DIDFTIALIKSKVGKLR 394 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 249 bits (635), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 155/378 (41%), Positives = 223/378 (58%), Gaps = 8/378 (2%) Query: 5 IYLDNNATTRPTPEVLAAMQACQHEAWGNPSSK-HRVGDAARQRMNSARAEVAALLAASA 63 IYLD +ATT P V M + GNP+S+ H G AA + AR +VAAL+ A Sbjct: 9 IYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNADV 68 Query: 64 AELVFTSGATEANHMAILGALA-LQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWIGV 122 E+++TSGATE+N++AI GA L+ + RH+++ EH + L +R L+ +G ++++ V Sbjct: 69 REIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFEVTYLEV 128 Query: 123 DEHGQLQLDELAAAITSDTALVSLMWANNETGVVFPIVQAAELARQRGVLFHTDAVQAMG 182 +G L L AA+ DT L S+M+ NNE GV+ I ++ R RGVL H DA QA G Sbjct: 129 GSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQATG 188 Query: 183 RVPCDVAGMGVDLLSLSAHKLHGPKGIGALYV--RKGIKLPPLLF-GHQERGRRGGTEDV 239 +V D+A + VDL+S SAHK +GPKGIGALYV + ++L + G ERG R GT Sbjct: 189 KVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLRSGTLAT 248 Query: 240 AGIVGLGVAADQARAAL-PRMLAVSALRQRFERGLACLPQVQINGAGAARLPNTVSVRFG 298 IVG+G A + AR A+ + LR R G A + + +NG R+P+ ++V F Sbjct: 249 HQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHNLNVSFN 308 Query: 299 QVHAERVLDRLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAAATIRFSLSRDTRED 358 V E +L + + + SSGSACT++ PS+VL A+GMS E A ++IRFS+ R T + Sbjct: 309 YVEGESLLMAIKDVAV--SSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRFTTVE 366 Query: 359 EIDAVLAALSEIVQPLQA 376 EID + + E V L+A Sbjct: 367 EIDFTVNVIREKVGKLRA 384 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 238 bits (608), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 154/379 (40%), Positives = 219/379 (57%), Gaps = 18/379 (4%) Query: 5 IYLDNNATTRPTPEVLAAMQACQHEAWGNP-SSKHRVGDAARQRMNSARAEVAALLAASA 63 IYLD ATT P+V M + +GN SS H G AR+ + ARA+VA L+ A Sbjct: 17 IYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRAVEQARAQVAGLVGAQP 76 Query: 64 AELVFTSGATEANHMAILGA-LALQPQRR--HIVSSLVEHPSTLLLLRHLQAQGVRISWI 120 E+V+TSGATE+N++A+ GA AL+ Q R H+++ EH + L + L+ G ++++ Sbjct: 77 GEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVLDTMHTLEKAGFEVTYL 136 Query: 121 GVDEHGQLQLDELAAAITSDTALVSLMWANNETGVVFPIVQAAELARQRGVLFHTDAVQA 180 G + +L AA+ DT L S+M+ NNE GV+ PI + E+ V+FH DAVQA Sbjct: 137 QPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGEICAAHDVVFHVDAVQA 196 Query: 181 MGRVPCDVAGMGVDLLSLSAHKLHGPKGIGALYVRKGIKLP---PLLFGHQERGRRGGTE 237 G++ D+ + VDL+S SAHK++GPKGIGALYVR +L + G E G R GT Sbjct: 197 AGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGHENGMRSGTL 256 Query: 238 DVAGIVGLGVAADQARAALPRMLA-----VSALRQRFERGLACLPQVQINGAGAARLPNT 292 IVG+G +A A R+L + ALR R GLA LP VQ+NG R+P+ Sbjct: 257 ATHQIVGMG----EAFALAGRLLEEESRRIGALRDRLWAGLADLPGVQLNGCARQRVPHN 312 Query: 293 VSVRFGQVHAERVLDRLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAAATIRFSLS 352 ++V F + +L LGI S+GSAC ++ PS VL+A+G S + A +RFSL Sbjct: 313 LNVSFPPTRHGTLGSQL--LGIAVSAGSACNSAAAAPSFVLLAIGRSPDVARNAVRFSLG 370 Query: 353 RDTREDEIDAVLAALSEIV 371 R T E EID V+A + +++ Sbjct: 371 RGTTEAEIDYVVATVRQVL 389 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 99.0 bits (245), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 107/391 (27%), Positives = 168/391 (42%), Gaps = 37/391 (9%) Query: 4 IIYLDNNATT-RPTPEVLAAMQACQHEAWGNPSSKHRVGDAARQRMNSARAEVAALL-AA 61 +I+ DN ATT +P + + + E + H + A AR V A L A Sbjct: 248 LIWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAP 307 Query: 62 SAAELVFTSGATEANHMAI--LGALALQPQRRHIVSSLVEHPSTLLLLRHLQAQ-GVRIS 118 E++F G TEA ++ G + IVS L EH + ++ + L A+ G ++ Sbjct: 308 DVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHL-EHHANIVPWQQLAAEKGAKLR 366 Query: 119 WIGVDEHGQLQLDELAAAITSDTALVSLMWANNETGVVFPIVQAAELARQRGVLFHTDAV 178 I VD+ GQ+ LDE A + T +V++ +N G + P Q ELAR G D Sbjct: 367 VIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLVDGA 426 Query: 179 QAMGRVPCDVAGMGVDLLSLSAHKLHGPKGIGALYVRKGI--KLPPL------------- 223 Q++ + DV +G D S HK+ GP GIGAL+ R+ + +PP Sbjct: 427 QSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNMIADVTFE 486 Query: 224 --LFGHQERGRRGGTEDVAGIVGLGVAADQA-RAALPRMLAVS-ALRQRFERGLACLPQV 279 +F GT ++A VGLG A D R + + A AL + L + + Sbjct: 487 KTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQLGEIEGL 546 Query: 280 QINGAGAARLPNTVSVRFGQVHAERVLDRLDRLGICASSGSACTASGDMPSHVLMAMGMS 339 ++ G AA + +S E V L++ GI +G C +L G+ Sbjct: 547 RLIGT-AAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQP------ILRRFGVE 599 Query: 340 SEQAAATIRFSLSRDTREDEIDAVLAALSEI 370 + T+R SL+ +EID +L + + Sbjct: 600 T-----TVRPSLAFYNTFEEIDCLLTVVRRL 625 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,710 Number of extensions: 45339 Number of successful extensions: 182 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 166 Number of HSP's successfully gapped: 5 Length of query: 379 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 292 Effective length of database: 1,207,925 Effective search space: 352714100 Effective search space used: 352714100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)