BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2325.p (376 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspir... 759 0.0 gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Her... 76 5e-15 gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Her... 74 2e-14 >gi|300312143|ref|YP_003776235.1| homocitrate synthase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 759 bits (1959), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/376 (98%), Positives = 375/376 (99%) Query: 1 MVTINDTTLRDGEQTAGVAFTAEEKIAIARALDEIGVPEMEIGIPIMGEAEIEVIQAIAA 60 MVTINDTTLRDGEQTAGVAFTAEEKI IARALDEIGVPEMEIGIPIMG+AEIEVIQAIAA Sbjct: 1 MVTINDTTLRDGEQTAGVAFTAEEKIDIARALDEIGVPEMEIGIPIMGQAEIEVIQAIAA 60 Query: 61 LPLRSKTMVWGRMCEADLAAAARCNADIVNLSMPVSDIHIQHKLQRDRDWVLAQIRYFLP 120 LPLRSKTMVWGRMCEADLAAAARCNADIVNLSMPVSDIHIQHKLQRDRDWVLAQIR+FLP Sbjct: 61 LPLRSKTMVWGRMCEADLAAAARCNADIVNLSMPVSDIHIQHKLQRDRDWVLAQIRHFLP 120 Query: 121 KALDLGMEVCLGGEDSSRADLDFMLRVIETAQAAGARRFRFADTLGLLDPFATYEVIRQL 180 KALDLGMEVCLGGEDSSRADLDFMLRVIETAQAAGARRFRFADTLGLLDPFATYEVIRQL Sbjct: 121 KALDLGMEVCLGGEDSSRADLDFMLRVIETAQAAGARRFRFADTLGLLDPFATYEVIRQL 180 Query: 181 RQEVDIELEMHAHNDLGLATANTLAAINAGASHVNTTVNGLGERAGNAPLEEVVMGLRHL 240 RQEVDIELEMHAHNDLGLATANTLAAINAGASHVNTTVNGLGERAGNAPLEEVVMGLRHL Sbjct: 181 RQEVDIELEMHAHNDLGLATANTLAAINAGASHVNTTVNGLGERAGNAPLEEVVMGLRHL 240 Query: 241 HGIESQVDTRSLPAISRLVAKASGKAVAPNKSIVGEAVFTHESGIHVDGLLKNPANYEHF 300 HGIE+QVDTRSLPAISRLVAKASGKAVAPNKSIVGEAVFTHESGIHVDGLLKNPANYEHF Sbjct: 241 HGIETQVDTRSLPAISRLVAKASGKAVAPNKSIVGEAVFTHESGIHVDGLLKNPANYEHF 300 Query: 301 SPAELGRSHHIVLGKHSGSRGVKDAFAKMGILLSDSQAQAMLLRIRDHTMVAKAAPSEDE 360 SPAELGRSHHIVLGKHSGSRGVKDAFAKMGILLSDSQAQAML+RIRDHTMVAKAAPSEDE Sbjct: 301 SPAELGRSHHIVLGKHSGSRGVKDAFAKMGILLSDSQAQAMLMRIRDHTMVAKAAPSEDE 360 Query: 361 LTRFFLESGACAASQM 376 LTRFFLESGACAASQM Sbjct: 361 LTRFFLESGACAASQM 376 >gi|300312728|ref|YP_003776820.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 567 Score = 75.9 bits (185), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 92/349 (26%), Positives = 141/349 (40%), Gaps = 39/349 (11%) Query: 7 TTLRDGEQTAGVAFTAEEKIAIARALDEIGVPEMEIGIPIMGEAEIEVIQAIAA---LP- 62 T LRDG Q AE K+ L + G+ E+E+G P + + + ++ + +P Sbjct: 37 TDLRDGNQALIEPMNAERKLRFFELLLKTGLKEIEVGFPSASQTDFDFVRKLIVENRIPD 96 Query: 63 -------LRSKTMVWGRMCEADLAAAARCNADIVNLSMPVSDIHIQHKLQRDRDWVLAQI 115 +S+ + R E+ L A + + N P I + R+ +A Sbjct: 97 DVTIIVLTQSREELIRRTIES-LEGAKKAIVHLYNSVAPAFR-KIVFNMSREEIKNIAVT 154 Query: 116 RYFLPKALD---LGMEVCL--GGEDSSRADLDFMLR----VIETAQAAGARR--FRFADT 164 L K L G E E S +LDF V ET A+ R+ T Sbjct: 155 GTRLVKELTDARPGTEWRFEYSPESFSTTELDFSKEICDAVCETWGASPERKVILNLPST 214 Query: 165 LGLLDPFATYEVI----RQLRQEVDIELEMHAHNDLGLATANTLAAINAGASHVNTTVNG 220 + P + I R L+ + +H HND G A A+ A+ AGA V + G Sbjct: 215 VECATPNVYADQIEWMCRNLKDRASTIISVHPHNDRGTAVASAELAVMAGADRVEGCLFG 274 Query: 221 LGERAGNAPLEEVVMGLRHLHGIESQVDTRSLPAISRLVAKASGKAVAPNKSIVGEAVFT 280 GER GN L + + L + G+ +D + + ++V + + V P VG+ VFT Sbjct: 275 NGERTGNVDLVTLALNL-YTQGVNPGLDFSDIDVVRQVVEECNQIPVHPRHPYVGDLVFT 333 Query: 281 HESGIHVDGLLKNPAN----------YEHFSPAELGRSHHIVLGKHSGS 319 SG H D + K A Y PA+LGRS+ V+ +S S Sbjct: 334 AFSGSHQDAIKKGFAKQQPDAIWEVPYLPIDPADLGRSYDAVIRVNSQS 382 >gi|300310272|ref|YP_003774364.1| 2-isopropylmalate synthase [Herbaspirillum seropedicae SmR1] Length = 575 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 90/361 (24%), Positives = 141/361 (39%), Gaps = 55/361 (15%) Query: 7 TTLRDGEQTAGVAFTAEEKIAIARALDEIGVPEMEIGIPIMGEAEIEVIQAIA---ALPL 63 T LRDG Q E K+ + L IG E+E+G P + E ++ + +P Sbjct: 36 TDLRDGNQALFEPMNRERKLRLFHELVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPD 95 Query: 64 RSKTMVWGRMCEADL--------AAAARCNADIVN----------LSMPVSDI------H 99 MV ++ ADL A A R N M V I H Sbjct: 96 DVTPMVMTQL-RADLITETVKSVAGARRVIVHFYNAIAPAWREIVFGMSVPQIITLVEDH 154 Query: 100 IQ-----HKLQRDRDWVLA-QIRYFLPKALDLGMEVCLGGEDSSRADLDFMLRVIETAQA 153 I + + +W+L F L++ +EVC + A + + Sbjct: 155 IALFRRLTAVHPETEWILQYSPETFCMAELEVSLEVCNAAIRAWDAGPRRRMIINLPTTV 214 Query: 154 AGARRFRFADTLGLLDPFATYEVIRQLRQEVDIELEMHAHNDLGLATANTLAAINAGASH 213 + FAD + +D R+L + + L +H HND G A A A+ AGA Sbjct: 215 EVSTPNVFADQIEWMD--------RRLERREHLILSVHPHNDRGTAVACAEQAMLAGAQR 266 Query: 214 VNTTVNGLGERAGNAPLEEVVMGLRHLHGIESQVDTRSLPAISRLVAKASGKAVAPNKSI 273 V + G GER+GN + + + L + GI +D + A++R+ + + + P Sbjct: 267 VEGCLFGNGERSGNVDVVTLALNL-YTQGIAPGLDFSDIAALARVAEECTALPIHPRHPY 325 Query: 274 VGEAVFTHESGIHVDGLLKNPA----------NYEHFSPAELGRSHHIV--LGKHSGSRG 321 VG+ VFT SG H D + K A Y P +LGR++ + + SG G Sbjct: 326 VGDLVFTAFSGSHQDAIAKGFAAQRPDAPWRVPYLPIDPTDLGRTYDSIVRVNSQSGKGG 385 Query: 322 V 322 + Sbjct: 386 I 386 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 984,654 Number of extensions: 35857 Number of successful extensions: 133 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 3 Length of query: 376 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 289 Effective length of database: 1,207,925 Effective search space: 349090325 Effective search space used: 349090325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)