BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2319.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 820 0.0 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 652 0.0 gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 470 e-134 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 228 9e-61 gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 96 6e-21 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 820 bits (2117), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/404 (98%), Positives = 402/404 (99%) Query: 4 SSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAA 63 SSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAA Sbjct: 3 SSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAA 62 Query: 64 LVNADVREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFE 123 LVNADVREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFE Sbjct: 63 LVNADVREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFE 122 Query: 124 VTYLEVGSDGLLDLVAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVD 183 VTYLEVGS+GLLDL AFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVD Sbjct: 123 VTYLEVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVD 182 Query: 184 AAQATGKVAIDLASLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLR 243 AAQATGKVAIDLA LKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLR Sbjct: 183 AAQATGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLR 242 Query: 244 SGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHN 303 SGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHN Sbjct: 243 SGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHN 302 Query: 304 LNVSFNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRF 363 LNVSFNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRF Sbjct: 303 LNVSFNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRF 362 Query: 364 TTIEEIDFTVNVIREKVGKLRALSPLWDMYKDGIDLSTVQWAAH 407 TT+EEIDFTVNVIREKVGKLRALSPLWDMYKDG+DLSTVQWAAH Sbjct: 363 TTVEEIDFTVNVIREKVGKLRALSPLWDMYKDGVDLSTVQWAAH 406 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 652 bits (1682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/400 (77%), Positives = 347/400 (86%) Query: 8 LPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNA 67 PIY+DYSATTPVDPRVA KMIPYL + GNPASRSH +GW AE AVE ARE+VA LVNA Sbjct: 18 FPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEVAKLVNA 77 Query: 68 DVREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFEVTYL 127 D REIIWTSGATESNNLAIKGAA+F +SKG+H+ITVKTEHKAVLDT RELER GFE TYL Sbjct: 78 DSREIIWTSGATESNNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTFRELERIGFEATYL 137 Query: 128 EVGSDGLLDLVAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQA 187 + +GL+ + AA+RPDTIL SVM VNNEIGVIQ + +G+ CR++G++ H DAAQA Sbjct: 138 QPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGIIFHSDAAQA 197 Query: 188 TGKVAIDLASLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLRSGTL 247 TGKV IDL + KVDLMSFSAHK+YGPKGIGALYV RKPRVR+EAQMHGGGHERGLRSGTL Sbjct: 198 TGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGHERGLRSGTL 257 Query: 248 ATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHNLNVS 307 ATHQIVGMGEAF LAR M +E RIR LRDRL G +IEE ++NGD+E RVPHNLNVS Sbjct: 258 ATHQIVGMGEAFRLAREEMDSELARIRALRDRLAKGLQEIEETYINGDMEHRVPHNLNVS 317 Query: 308 FNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRFTTIE 367 FNYVEGESL+MAIKD+AVSSGSACTSASLEPSYVLRALG SDELAHSSIRF+IGRFTT E Sbjct: 318 FNYVEGESLIMAIKDIAVSSGSACTSASLEPSYVLRALGRSDELAHSSIRFTIGRFTTEE 377 Query: 368 EIDFTVNVIREKVGKLRALSPLWDMYKDGIDLSTVQWAAH 407 +IDFT+ +I+ KVGKLR LSPLWDMYKDG+DLST+QWAAH Sbjct: 378 DIDFTIALIKSKVGKLRELSPLWDMYKDGVDLSTIQWAAH 417 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 470 bits (1210), Expect = e-134, Method: Compositional matrix adjust. Identities = 241/379 (63%), Positives = 283/379 (74%), Gaps = 2/379 (0%) Query: 2 PSSSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQV 61 P + L PIYLDY ATTPVDP+VA+ M +LTD GN AS SH FGW A AVE AR QV Sbjct: 9 PPADLRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRAVEQARAQV 68 Query: 62 AALVNADVREIIWTSGATESNNLAIKGAAEFLRSKGR--HLITVKTEHKAVLDTMRELER 119 A LV A EI+WTSGATESNNLA+KGAA LR++GR HLITV TEHKAVLDTM LE+ Sbjct: 69 AGLVGAQPGEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVLDTMHTLEK 128 Query: 120 RGFEVTYLEVGSDGLLDLVAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVL 179 GFEVTYL+ G+DGL+ AALRPDT+LASVMYVNNEIGVIQ IA LG+IC A V+ Sbjct: 129 AGFEVTYLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGEICAAHDVV 188 Query: 180 LHVDAAQATGKVAIDLASLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHE 239 HVDA QA GK+AIDL LKVDLMSFSAHK YGPKGIGALYV P+++LEAQ+ GGGHE Sbjct: 189 FHVDAVQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGHE 248 Query: 240 RGLRSGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERR 299 G+RSGTLATHQIVGMGEAF LA + E+ RI LRDRL AG AD+ +NG +R Sbjct: 249 NGMRSGTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGLADLPGVQLNGCARQR 308 Query: 300 VPHNLNVSFNYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGMSDELAHSSIRFS 359 VPHNLNVSF +L + +AVS+GSAC SA+ PS+VL A+G S ++A +++RFS Sbjct: 309 VPHNLNVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLAIGRSPDVARNAVRFS 368 Query: 360 IGRFTTIEEIDFTVNVIRE 378 +GR TT EID+ V +R+ Sbjct: 369 LGRGTTEAEIDYVVATVRQ 387 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 228 bits (580), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 157/378 (41%), Positives = 224/378 (59%), Gaps = 8/378 (2%) Query: 10 IYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNADV 69 IYLD +ATT P V M + GNP+S+ H G AA + AR +VAAL+ A Sbjct: 5 IYLDNNATTRPTPEVLAAMQACQDEAWGNPSSK-HRVGDAARQRMNSARAEVAALLAASA 63 Query: 70 REIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELERRGFEVTYLEV 129 E+++TSGATE+N++AI GA L+ + RH+++ EH + L +R L+ +G ++++ V Sbjct: 64 AELVFTSGATEANHMAILGALA-LQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWIAV 122 Query: 130 GSDGLLDLVAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQATG 189 G L L AA+ PDT L S+M+ NNE GV+ IA ++ R RGVL H DA QA G Sbjct: 123 DEHGQLQLDELAAAITPDTALVSLMWANNETGVVFPIAQAAELARQRGVLFHTDAVQAVG 182 Query: 190 KVAIDLASLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHERGLRSGTLAT 249 +V D+A L VDL+S SAHK +GPKGIGALYV + ++L + G ERG R GT Sbjct: 183 RVPCDVAGLGVDLLSLSAHKLHGPKGIGALYV--RKGIKLPPLLF-GHQERGRRGGTEDV 239 Query: 250 HQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRVPHNLNVSFN 309 IVG+G A AR A+ + + LR R G A + + +NG R+P+ ++V F Sbjct: 240 AGIVGLGVAAAQARAAL-PRMQAVSALRQRFERGLARLPQVKINGAGAARLPNTVSVRFG 298 Query: 310 YVEGESLLMAIKDVAV--SSGSACTSASLEPSYVLRALGMSDELAHSSIRFSIGRFTTIE 367 V E +L + + + SSGSACT++ PS+VL A+GMS E A ++IRFS+ R T + Sbjct: 299 QVHAELVLERLDRLGICASSGSACTASGDMPSHVLMAMGMSSEQAVATIRFSLSRDTRED 358 Query: 368 EIDFTVNVIREKVGKLRA 385 EID + + + V L+A Sbjct: 359 EIDAVLAALLQIVQPLQA 376 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 95.9 bits (237), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 104/393 (26%), Positives = 165/393 (41%), Gaps = 43/393 (10%) Query: 10 IYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNA-D 68 I+ D +ATT V ++ + N +H A A E ARE+V A +NA D Sbjct: 249 IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 308 Query: 69 VREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELE-RRGFEVTYL 127 V E+I+ G TE+ NL K G +I EH A + ++L +G ++ + Sbjct: 309 VNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLRVI 368 Query: 128 EVGSDGLLDLVAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQA 187 V G + L + L T + +V V+N +G I + ++ R G VD AQ+ Sbjct: 369 PVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGARTLVDGAQS 428 Query: 188 TGKVAIDLASLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGH--------- 238 + D+ +L D FS HK +GP GIGAL+ GR+ + GGG+ Sbjct: 429 VSHMRTDVQALGADFFVFSGHKVFGPTGIGALW-GRRDVLEDMPPWQGGGNMIADVTFEK 487 Query: 239 ------ERGLRSGTLATHQIVGMGEAFELARLAMGTEN---ERIRMLRDRLHAGFADIEE 289 +GT VG+G A + +G +N +L +H +IE Sbjct: 488 TIFQPLPNTFEAGTGNIADAVGLGAAIDYVN-RVGIDNIAAYEHALLEYGMHQ-LGEIEG 545 Query: 290 CFVNGDLERRVPHNLNVSFNYVEGESL-----LMAIKDVAVSSGSACTSASLEPSYVLRA 344 + G H +V +EG S + + +AV +G C +LR Sbjct: 546 LRLIGTAA----HKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQP------ILRR 595 Query: 345 LGMSDELAHSSIRFSIGRFTTIEEIDFTVNVIR 377 G+ +++R S+ + T EEID + V+R Sbjct: 596 FGVE-----TTVRPSLAFYNTFEEIDCLLTVVR 623 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,245,155 Number of extensions: 51825 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 5 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)