BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2308.p (628 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 1117 0.0 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 102 8e-23 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 90 5e-19 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 86 8e-18 gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 80 4e-16 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 1117 bits (2889), Expect = 0.0, Method: Compositional matrix adjust. Identities = 583/632 (92%), Positives = 604/632 (95%), Gaps = 4/632 (0%) Query: 1 VSILTPASHSAGLPGQDASAAPFDPALLARLANELFAAPAAPVWSPGQVPGAQPPANFTP 60 +SILTPAS GLPGQDASAAPFDPALLARLANELFAAP AP W+PGQ PGAQPPANFTP Sbjct: 1 MSILTPASQGGGLPGQDASAAPFDPALLARLANELFAAPTAPAWTPGQAPGAQPPANFTP 60 Query: 61 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD 120 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD Sbjct: 61 PASPLSSPAGTSQGVPGTAIPQGLAPGLNLIPSSPHQVLTLGNRAPALAPAAPAGNGTPD 120 Query: 121 KVFAALPAYEPRVGGAVTGAPQAVAAAPAAAPSTAGYYFLDDGHGHPRGGASTAPTAAPA 180 KVFAALPAYEPR+GGAVTGAPQA A + AAPSTAGYYFLD GHGHPRG A+ A + Sbjct: 121 KVFAALPAYEPRLGGAVTGAPQAAAPSTPAAPSTAGYYFLDGGHGHPRGSAAAPAAAPLS 180 Query: 181 VPASTS----QYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQTAAFDVHAVRRDFSILQ 236 ++ + QYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQ AAFDVH VRRDF ILQ Sbjct: 181 SSSAGAASSPQYYFLHAVELPSGYVTPAKPAPHAVHQPAGAQQAAAFDVHTVRRDFPILQ 240 Query: 237 ERVNGRPLVWFDNAATTHKPQAVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV 296 ERVNGRPL+WFDNAATTHKPQ+VIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV Sbjct: 241 ERVNGRPLIWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERV 300 Query: 297 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE 356 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE Sbjct: 301 RAFLNAPDVNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAE 360 Query: 357 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTVTPTKQIVELARLAGAR 416 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGT+TPTKQIVELARLAGAR Sbjct: 361 KGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQIVELARLAGAR 420 Query: 417 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRREVLEDMPPWQGGGNMI 476 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRR+VLEDMPPWQGGGNMI Sbjct: 421 TLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRDVLEDMPPWQGGGNMI 480 Query: 477 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRIGIDNIASYEHALLEYGMHKL 536 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNR+GIDNIA+YEHALLEYGMH+L Sbjct: 481 ADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIAAYEHALLEYGMHQL 540 Query: 537 GEVDGLRLIGTAAHKASVMSFVLDGYSTEEVGKALNQEGIAVRTGHHCAQPILRRFGVET 596 GE++GLRLIGTAAHKASVMSFVL+GYSTE+VGKALNQEGIAVRTGHHCAQPILRRFGVET Sbjct: 541 GEIEGLRLIGTAAHKASVMSFVLEGYSTEDVGKALNQEGIAVRTGHHCAQPILRRFGVET 600 Query: 597 TVRPSLAFYNTFEEIDCLLTVVRRLSAQRHRR 628 TVRPSLAFYNTFEEIDCLLTVVRRL+AQRHRR Sbjct: 601 TVRPSLAFYNTFEEIDCLLTVVRRLAAQRHRR 632 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 102 bits (254), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 100/388 (25%), Positives = 169/388 (43%), Gaps = 33/388 (8%) Query: 245 VWFDNAATTHKPQAVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 304 ++ D +ATT V D++ + R + N +H A A E ARE V +NA D Sbjct: 20 IYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEVAKLVNA-D 78 Query: 305 VNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLRVI 364 E+I+ G TE+ NL K + G II EH A + +++L G + + Sbjct: 79 SREIIWTSGATESNNLAIKGAAQFYKSKGKHIITVKTEHKAVLDTFRELE-RIGFEATYL 137 Query: 365 PVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTVTPTKQIVELARLAGARTLVDGAQS 424 D+G V +++ A + T +V+V V+N +G + P +I E R G D AQ+ Sbjct: 138 QPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGIIFHSDAAQA 197 Query: 425 VSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRE-VLEDMPPWQGGGNMIADVTFEK 483 ++ D++ D FS HK +GP GIGAL+ RR+ + GGG+ Sbjct: 198 TGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGH--------- 248 Query: 484 TIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRIGIDNIASYEHALLEYGMHKLGEVDGLR 543 +GT VG+G A + R +D+ + AL + L E++ Sbjct: 249 ------ERGLRSGTLATHQIVGMGEAF-RLAREEMDSELARIRALRDRLAKGLQEIEETY 301 Query: 544 LIGTAAHKA-SVMSFVLDGYSTEEVGKALNQEGIAVRTGHHCAQP------ILRRFG--- 593 + G H+ ++ + E + A+ IAV +G C +LR G Sbjct: 302 INGDMEHRVPHNLNVSFNYVEGESLIMAIKD--IAVSSGSACTSASLEPSYVLRALGRSD 359 Query: 594 --VETTVRPSLAFYNTFEEIDCLLTVVR 619 +++R ++ + T E+ID + +++ Sbjct: 360 ELAHSSIRFTIGRFTTEEDIDFTIALIK 387 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 90.1 bits (222), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 104/403 (25%), Positives = 164/403 (40%), Gaps = 63/403 (15%) Query: 245 VWFDNAATTHKPQAVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 304 ++ D +ATT V ++ + N +H A A E ARE+V A +NA D Sbjct: 9 IYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVAALVNA-D 67 Query: 305 VNEVIFVRGTTEAINLVAKSWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLRVI 364 V E+I+ G TE+ NL K G +I EH A + ++L +G ++ + Sbjct: 68 VREIIWTSGATESNNLAIKGAAEFLRSKGRHLITVKTEHKAVLDTMRELE-RRGFEVTYL 126 Query: 365 PVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTVTPTKQIVELARLAGARTLVDGAQS 424 V +G + L + L T + +V V+N +G + + ++ R G VD AQ+ Sbjct: 127 EVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLLHVDAAQA 186 Query: 425 VSHMRTDVQALGADFFVFSGHKVFGPTGIGALW-GRREVLEDMPPWQGGGNMIADVTFEK 483 + D+ L D FS HK +GP GIGAL+ GR+ + GGG+ Sbjct: 187 TGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGH--------- 237 Query: 484 TIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRIGIDNIASYEHALLEYGMHKLGEVDGLR 543 +GT VG+G A +E A L G E + +R Sbjct: 238 ------ERGLRSGTLATHQIVGMGEA--------------FELARLAMGT----ENERIR 273 Query: 544 LIGTAAHK--ASVMSFVLDGYSTEEVGKALN-----QEG---------IAVRTGHHCAQP 587 ++ H A + ++G V LN EG +AV +G C Sbjct: 274 MLRDRLHAGFADIEECFVNGDLERRVPHNLNVSFNYVEGESLLMAIKDVAVSSGSACTSA 333 Query: 588 ------ILRRFGVE-----TTVRPSLAFYNTFEEIDCLLTVVR 619 +LR G+ +++R S+ + T EEID + V+R Sbjct: 334 SLEPSYVLRALGMSDELAHSSIRFSIGRFTTVEEIDFTVNVIR 376 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 85.9 bits (211), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 84/293 (28%), Positives = 129/293 (44%), Gaps = 34/293 (11%) Query: 336 IIVSHLEHHANIVPWQQLAAEKGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSN 395 I+ S +EH + ++ + L A+ G ++ I VD+ GQ+ LDE A + T +V++ +N Sbjct: 93 IVSSLVEHPSTLLLLRHLQAQ-GVRISWIAVDEHGQLQLDELAAAITPDTALVSLMWANN 151 Query: 396 ALGTVTPTKQIVELARLAGARTLVDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGA 455 G V P Q ELAR G D Q+V + DV LG D S HK+ GP GIGA Sbjct: 152 ETGVVFPIAQAAELARQRGVLFHTDAVQAVGRVPCDVAGLGVDLLSLSAHKLHGPKGIGA 211 Query: 456 LWGRREVLEDMPPWQGGGNMIADVTFEKTIFQPLPNTFEAGTGNIADAVGLGA-AIDYVN 514 L+ R+ + +PP +F GT ++A VGLG A Sbjct: 212 LYVRKGI--KLPPL---------------LFGHQERGRRGGTEDVAGIVGLGVAAAQARA 254 Query: 515 RIGIDNIASYEHALLEYGMHKLGEVDGLRLIGT-AAHKASVMSFVLDGYSTEEVGKALNQ 573 + S E G+ +L +V ++ G AA + +S E V + L++ Sbjct: 255 ALPRMQAVSALRQRFERGLARLPQV---KINGAGAARLPNTVSVRFGQVHAELVLERLDR 311 Query: 574 EGIAVRTGHHCAQP------ILRRFGVET-----TVRPSLAFYNTFEEIDCLL 615 GI +G C +L G+ + T+R SL+ +EID +L Sbjct: 312 LGICASSGSACTASGDMPSHVLMAMGMSSEQAVATIRFSLSRDTREDEIDAVL 364 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 80.5 bits (197), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 103/397 (25%), Positives = 163/397 (41%), Gaps = 39/397 (9%) Query: 245 VWFDNAATTHKPQAVIDRISEFYRRENSNIHRAAHELAARATDAYEGARERVRAFLNAPD 304 ++ D ATT V + +F + N ++H A A E AR +V + A Sbjct: 17 IYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPFGWEARRAVEQARAQVAGLVGAQP 76 Query: 305 VNEVIFVRGTTEAINLVAK--SWGGKHVGAGDEIIVSHLEHHANIVPWQQLAAEKGAKLR 362 E+++ G TE+ NL K ++ + G G+ +I EH A + L + G ++ Sbjct: 77 -GEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVPTEHKAVLDTMHTLE-KAGFEVT 134 Query: 363 VIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTVTPTKQIVELARLAGARTLVDGA 422 + G + + L T + +V V+N +G + P ++ E+ VD Sbjct: 135 YLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQPIAELGEICAAHDVVFHVDAV 194 Query: 423 QSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRE-VLEDMPPWQGGGNMIADVTF 481 Q+ + D+ L D FS HKV+GP GIGAL+ R L+ GGG+ Sbjct: 195 QAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHNPQLKLEAQIDGGGH------- 247 Query: 482 EKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRIGIDNIASYEHALLEYGMHKLGEVDG 541 N +GT VG+G A R+ ++ + AL + L ++ G Sbjct: 248 --------ENGMRSGTLATHQIVGMGEAFALAGRL-LEEESRRIGALRDRLWAGLADLPG 298 Query: 542 LRLIGTAAHKAS---VMSFVLDGYSTEEVGKALNQEGIAVRTGHHC----AQP--ILRRF 592 ++L G A + +SF + T +G L GIAV G C A P +L Sbjct: 299 VQLNGCARQRVPHNLNVSFPPTRHGT--LGSQL--LGIAVSAGSACNSAAAAPSFVLLAI 354 Query: 593 G-----VETTVRPSLAFYNTFEEIDCLLTVVRRLSAQ 624 G VR SL T EID ++ VR++ A Sbjct: 355 GRSPDVARNAVRFSLGRGTTEAEIDYVVATVRQVLAH 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,035,693 Number of extensions: 91606 Number of successful extensions: 456 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 450 Number of HSP's successfully gapped: 5 Length of query: 628 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 537 Effective length of database: 1,188,985 Effective search space: 638484945 Effective search space used: 638484945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)