BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2272.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309898|ref|YP_003773990.1| major facilitator superfamily p... 125 6e-30 gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum serope... 97 2e-21 gi|300309392|ref|YP_003773484.1| major facilitator superfamily (... 65 7e-12 >gi|300309898|ref|YP_003773990.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 392 Score = 125 bits (314), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 115/374 (30%), Positives = 178/374 (47%), Gaps = 14/374 (3%) Query: 11 LSGRALAVLSVAAGSAIANNYAMQPALSLIAADFGVPVAPMTALASAAIIGYLLGLVLLV 70 LS L + ++A+G ++AN Y QP L ++ DF + A + + +A +G L L+LLV Sbjct: 8 LSQGLLLLFAIASGLSVANVYYAQPLLDALSDDFKISQAAVGGVVTATQLGCALALLLLV 67 Query: 71 PLTDKLSPRVLIPGQLFALACALLLAAWAASPEVLIGCFVLIGATTTVAAQS-SAVVGKY 129 PL DK+ ++L+ QL AL CAL+ A S VL+ + +G T Q A Sbjct: 68 PLGDKVPRKMLMGIQLLALVCALVTVGLARSSYVLLAGMLAVGLLGTAMTQGLIAYAASA 127 Query: 130 AHAQHRALSMATISAGISAGILLSRFVGGVLAQWWGWRGALLAFAAFAAVSALCV----- 184 A R + +G+ G+LLSR G ++ GWRG L AA+ LC+ Sbjct: 128 AGEDERGRVVGAAQSGVFIGLLLSRVFSGAVSDVTGWRGVYLC----AAIMMLCLAWPLW 183 Query: 185 --LPLLPTQGPDAQSGYFSNLRSMPILMKTSRELRLRIYAGMLWFFAFNLIWVGLVVRLA 242 LP +P Q + Y + ++SM L++ + L++R ML F AFN+ W LV+ L+ Sbjct: 184 KRLPAIPVQADGLR--YLTLIKSMWCLLRDDKVLQVRGVLAMLMFAAFNVFWSALVLPLS 241 Query: 243 APPFNLDAAHIGLYSLAGLMGLLMTRVAGRLADRFGHRRVVVSGLTSAFFAAIGLALVLG 302 A P++ IG + L G++G L AGR +D+ +R L A L+L+ Sbjct: 242 ASPYHFSHTVIGAFGLVGVIGALAASKAGRWSDQGHAQRTSAVSLAVLLLAWWPLSLMES 301 Query: 303 HPAAIAVALAVFDAGCFTAQVANQAGVVAIEPSRAGVLNSAYLFFYYVAGTIGAALAGVI 362 A+ + + D G V NQ+ + P L Y+ FY V +GA I Sbjct: 302 SLWALVAGIILLDLGGQALHVTNQSLIFRSSPQAHSRLVGVYMLFYAVGSGLGAISTTAI 361 Query: 363 VGSAGWEALTLLAA 376 + GW+ + L A Sbjct: 362 YAARGWQGVCFLGA 375 >gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum seropedicae SmR1] Length = 412 Score = 97.4 bits (241), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 115/351 (32%), Positives = 164/351 (46%), Gaps = 5/351 (1%) Query: 23 AGSAIANNYAMQPALSLIAADFGVPVAPMTALASAAIIGYLLGLVLLVPLTDKLSPRVLI 82 AG A+A+ Y QP L ++A D G + + + +GY LG++LL PL D+ R +I Sbjct: 35 AGLAVASLYYSQPMLGVLADDIGASERAVGMVPMSTQLGYALGILLLAPLGDRYDRRRII 94 Query: 83 PGQLFALACALLLAAWAASPEVLIGCFVLIGATTTVAAQSSAVVGKYAHAQHRALSMATI 142 + L ALLLA A+ L+ + +G T T+A A Q R + T+ Sbjct: 95 LIKAAILTAALLLAGAASGVGALLAASLAVGLTATLAQDIVPAAASLAPEQSRGRVVGTV 154 Query: 143 SAGISAGILLSRFVGGVLAQWWGWRGALLAFAAFAAVSALCVLPLLPTQGPDAQSGYFSN 202 G+ GILLSR V G +A+ +GWR +A A A+ L LP P Y + Sbjct: 155 MTGLLLGILLSRVVSGFVAEHFGWRAMFVAAAVSIALIGLAAWRGLPRFHPTTHLSYGAL 214 Query: 203 LRSMPILMKTSRELRLRIYAGMLWFFAFNLIWVGLVVRLAAPPFNLDAAHIGLYSLAGLM 262 + S+ L K LR A L F+ W L V L PF+L +A G + LAG Sbjct: 215 MASLATLFKKHGALRRAALAQGLLSVGFSAFWSTLAVMLHGAPFHLGSAAAGAFGLAGAA 274 Query: 263 GLLMTRVAGRLADRFGHRRVVVSGLTSAF--FAAIGLA---LVLGHPAAIAVALAVFDAG 317 G L +AGR++D+ G V G+ A FAA+ L+ G +AVA FD G Sbjct: 275 GALAAPLAGRISDKKGPELVTRLGVGLAIVSFAAMALSPWFSTQGQLVLLAVAAVGFDLG 334 Query: 318 CFTAQVANQAGVVAIEPSRAGVLNSAYLFFYYVAGTIGAALAGVIVGSAGW 368 VA+Q V +IEP LN+ ++ GAAL +++ GW Sbjct: 335 VQATLVAHQTIVYSIEPGARSRLNAILFVGMFIGMAAGAALGSLVLAQWGW 385 >gi|300309392|ref|YP_003773484.1| major facilitator superfamily (MFS) transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 423 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 93/351 (26%), Positives = 146/351 (41%), Gaps = 19/351 (5%) Query: 31 YAMQPALSLIAADFGVPVAPMTALASAAIIGYLLGLVLLVPLTDKLSPRVLIPGQLFALA 90 Y +QP L + A +FG+ A + S + + +VL L+++ R L+ L A Sbjct: 58 YCVQPLLPIFAQEFGIGAASASLALSLSTGLLAVSIVLAGMLSERYGRRELMFASLATAA 117 Query: 91 CALLLAAWAASPEVLIGCFVLIG-ATTTVAAQSSAVVGKYAHAQHRALSMATISAGISAG 149 C L A A +L+ L G A V A + A + + A +M G + G Sbjct: 118 CCNLAAGVAPDWHLLLLARTLEGIALGGVPAVAMAYLAEEIDAAGLGFAMGLYVGGNAFG 177 Query: 150 ILLSRFVGGVLAQWWGWRGALLAFAAFAAVSALCVLPLLPTQGPDAQSGYFSNLRSMPIL 209 + R VLA+ GWR +L A A+ + +LP ++ + Sbjct: 178 GMAGRVAMSVLAEHLGWRAGMLIIGALDLALAVAFVMMLPASRHFVRNSSMRLADHFALW 237 Query: 210 MKTSRELRLRIYAGMLWFFAFNLIWVGLVV--------RLAAPPFNLDAAHIGLYSLAGL 261 R RL + FA + +G+ V RL +PPF+L A GL A L Sbjct: 238 RGHLRHARLPLC------FAIAALNMGVFVTSYNYAGFRLMSPPFSLSATVTGLIFSAYL 291 Query: 262 MGLLMTRVAGRLADRFGHRRVVVSGLTSAFFAAIGLALVLGHPAAIAV-ALAVFDAGCFT 320 G+L + AG LADR+G +V +G+ + +GL L + + + + G FT Sbjct: 292 FGMLSSSWAGALADRYGRAPLVAAGIG---VSCLGLLLTCTSSLVLVIGGIVLLTIGFFT 348 Query: 321 AQVANQAGVVAIEPSRAGVLNSAYLFFYYVAGTIGAALAGVIVGSAGWEAL 371 A + V + G S YL YY+ G+I ++ G AGW A+ Sbjct: 349 THSAASSWVGRMAREGKGHAASLYLLAYYLGGSILGSVGGWFWEHAGWNAV 399 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,211 Number of extensions: 34794 Number of successful extensions: 353 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 348 Number of HSP's successfully gapped: 6 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)