BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2255.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 961 0.0 gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 502 e-143 gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 70 3e-13 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 59 1e-09 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 961 bits (2483), Expect = 0.0, Method: Compositional matrix adjust. Identities = 487/501 (97%), Positives = 491/501 (98%) Query: 1 MKTINQLAAVPETNDGGADSGMSTAYCRARWGLLTASGLDETQLLAGVSRALRKGGDYAD 60 MKTINQLAAVPE NDGGADSG STAYCRARWGLL ASGLDE+QLLAGVSRALRKGGDYAD Sbjct: 1 MKTINQLAAVPEPNDGGADSGTSTAYCRARWGLLAASGLDESQLLAGVSRALRKGGDYAD 60 Query: 61 LYLKSSIAESWHLERGKVARSNYNQSQGFGLRLVEGEQSVLTSADRFDATAIGKAADLAA 120 LYLKSS+AESWHLERGKV RSNYNQSQGFGLRLVEGEQSVLTSADRFDAT IGKAADLAA Sbjct: 61 LYLKSSVAESWHLERGKVTRSNYNQSQGFGLRLVEGEQSVLTSADRFDATTIGKAADLAA 120 Query: 121 DAVGHVPVSPAGLVLRLPKHGPTYYTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEV 180 DAVGH PVSPAGLVL+LPK GPTYYTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEV Sbjct: 121 DAVGHAPVSPAGLVLKLPKRGPTYYTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEV 180 Query: 181 MASLSISLDLVFLARNDGYCGGDVRPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDR 240 MASLSISLDLVFLARNDGYCGGDVRPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFD Sbjct: 181 MASLSISLDLVFLARNDGYCGGDVRPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDH 240 Query: 241 TAIESVAALAVDAALIKLEARAAPAGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSA 300 AIESVAALAVDAALIKLEARAAPAGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSA Sbjct: 241 AAIESVAALAVDAALIKLEARAAPAGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSA 300 Query: 301 FAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNAR 360 FAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNAR Sbjct: 301 FAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNAR 360 Query: 361 LMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISS 420 LMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISS Sbjct: 361 LMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISS 420 Query: 421 GRFMFAASEAFLIEDGRLSAPVKGATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQS 480 GRFMFAASEAFLIE+GRL APVKGATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQS Sbjct: 421 GRFMFAASEAFLIENGRLGAPVKGATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQS 480 Query: 481 VPVGVGQPTLRIDGMTVGGTA 501 VPVGVGQPTLRIDGMTVGGTA Sbjct: 481 VPVGVGQPTLRIDGMTVGGTA 501 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 502 bits (1293), Expect = e-143, Method: Compositional matrix adjust. Identities = 261/477 (54%), Positives = 331/477 (69%), Gaps = 8/477 (1%) Query: 29 ARWGLLTASGLDETQLLAGVSRALRKGGDYADLYLKSSIAESWHLERGKVARSNYNQSQG 88 AR LLT GLDE++LL + DYADLY + + +E W LE G V +++ QG Sbjct: 14 ARDVLLTPFGLDESKLLKALGSMFTHKVDYADLYFQFTKSEGWSLEEGIVKTGSFSIDQG 73 Query: 89 FGLRLVEGEQSVLTSADRFDATAI----GKAADLAADAVGHVPVSPAGLVLRLPKHGPTY 144 G+R V G+++ + +D +A+ +A G + V+ A +P G Sbjct: 74 VGVRAVSGDKTAFSYSDEISESALMDAAAATRTIARQGSGKIKVAGA----MIPGGGRAL 129 Query: 145 YTLDDPLPSLDAAHKIALLESVDRACRARSPLVVEVMASLSISLDLVFLARNDGYCGGDV 204 Y DPL SLDA K+ LLE V+R RA+ P V +VMASLS D+V +AR DG D+ Sbjct: 130 YLPHDPLTSLDATAKVQLLERVERIARAKDPRVKQVMASLSGEYDVVLVARADGVLAADI 189 Query: 205 RPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDRTAIESVAALAVDAALIKLEARAAP 264 RPLVR+ V V +NGRRE S GGR F +E A+ AV AL+ LE+R AP Sbjct: 190 RPLVRVSVTVIAEQNGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRPAP 249 Query: 265 AGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPGVTIVDDGTVPG 324 AG MTVV+GPGW G++LHEA+GHGLEGDFNR+GSS F+GRIG+RVAA GVT+VDDGT+ Sbjct: 250 AGPMTVVLGPGWPGILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIAD 309 Query: 325 SRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNARLMGVANTGNGRRESYDVLPMPRMT 384 RGSLNIDDEG P QCTTLIEDGIL Y+QD++NARLM + TGN RRES+ LPMPRMT Sbjct: 310 RRGSLNIDDEGNPTQCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMT 369 Query: 385 NTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISSGRFMFAASEAFLIEDGRLSAPVKG 444 NTYM G DPAEI+ASVK G+Y + GGQVDI++G+F+F+ASEA++IEDG+++ PVKG Sbjct: 370 NTYMLGGDRDPAEILASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKG 429 Query: 445 ATVLGNGPQSLKNVKLIGNDLEINTASGVCGKAGQSVPVGVGQPTLRIDGMTVGGTA 501 AT++GNGP L V +IGND+ +++ GVCGK GQSVPVGVGQPTLR+DG+TVGGTA Sbjct: 430 ATLIGNGPDVLNRVSMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRLDGVTVGGTA 486 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 70.5 bits (171), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 94/333 (28%), Positives = 140/333 (42%), Gaps = 22/333 (6%) Query: 141 GPTYYTLDDPLPSLDAAHK------IALLESVDRACRARSPLVVEV-MASLSISLDLVFL 193 G + Y DD LD A + +AL+ ++RA A S V ++ A L S L Sbjct: 116 GLSAYPSDDVDLELDHAWQLGLRDAVALVARIERAALAVSSAVPQIEQAVLFSSRTRRVL 175 Query: 194 ARNDGYCGGDVRPLVRLDVAVQVVRNGRREGASRTLGGRVGLATFDRTAIESVAALAVDA 253 A ++G+ L +R+G +E S R A + ES+ A A Sbjct: 176 ANSEGFLRSSASTRHSLSCTAVAIRDGAKEIES-WWDARRDPAQL--PSPESLGACAGRR 232 Query: 254 ALIKLEARAAPAGTMTVVIGPGWNGVMLHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPG 313 AL +L AR T V+ P +L+E V + SS +GQ V A Sbjct: 233 ALARLGARQVATQTCPVLFEPQAAASLLNEFV-QAISARPLYMHSSFLRDALGQEVFAAH 291 Query: 314 VTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNARLMG--VANTGNGR 371 + I DD + G GS + D G + TLI G L Y + AR +G +A G G Sbjct: 292 MNISDDPLLKGGMGSRSYDGNGAALRPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAG- 350 Query: 372 RESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDISSGRFMFAASEAF 431 P M ++ A D +I + G+ I+ L G V++ +G F A S F Sbjct: 351 -------PSNVMVSSTTSAASGDLEAMIRRMGTGLVISGLNGQGVNLMTGDFSRACS-GF 402 Query: 432 LIEDGRLSAPVKGATVLGNGPQSLKNVKLIGND 464 +E+G+++ PV G TV N + + +GND Sbjct: 403 WVENGQIAYPVAGITVASNLREMFAGIVAVGND 435 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 58.9 bits (141), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 56/228 (24%), Positives = 100/228 (43%), Gaps = 16/228 (7%) Query: 244 ESVAALAVDAALIKLEARAAPAGTMTVV----IGPGWNGVMLHEAVGHGLEGDFNRRGSS 299 E++ A + AL +L AR V+ + G G + G L R S+ Sbjct: 220 EAIGRYAAERALARLNARKLSTRKCPVLFEAPLAAGLLGAFVQAVSGGAL-----YRKST 274 Query: 300 AFAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQCTTLIEDGILVSYMQDSLNA 359 +GQ+V + I++D V G GS D+EG ++ +G++ Y + +A Sbjct: 275 FLLDSLGQQVFPEHIQILEDPHVIGGVGSAPFDEEGVRTLKREVVRNGVVQGYFLSTYSA 334 Query: 360 RLMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIASVKQGIYIAHLEGGQVDIS 419 R +G+ TGN S+++ +T G ++ + G+ + L G V+ Sbjct: 335 RKLGMQTTGNS-GGSHNLTLRSELTTRADTFKG-----MLKKMGTGLLVTELMGQGVNYV 388 Query: 420 SGRFMFAASEAFLIEDGRLSAPVKGATVLGNGPQSLKNVKLIGNDLEI 467 +G + AS + +E+G + PV+ T+ GN L+ + +GND I Sbjct: 389 TGDYSRGAS-GYWVENGVIQYPVEEITIAGNMKDMLRQIVAVGNDTLI 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,672,416 Number of extensions: 75511 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 4 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)