BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.2254.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE prot... 893 0.0 gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspi... 326 2e-90 gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD prot... 71 2e-13 gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspi... 61 2e-10 >gi|300312124|ref|YP_003776216.1| Zn-dependent protease TLDE protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 893 bits (2307), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/457 (96%), Positives = 450/457 (98%) Query: 1 MRSLRAIRNLNTMKDCQQYFETIAQQTLEVARRHGATAAAVQIEEVESLSASVQGGRPNE 60 M+SLRA+RNLNTMKDCQQ+FETIAQQTLEVAR+HGATAAAVQIEEVESLSASVQGG+PNE Sbjct: 1 MKSLRALRNLNTMKDCQQHFETIAQQTLEVARQHGATAAAVQIEEVESLSASVQGGKPNE 60 Query: 61 RALRQSTQLRLSLFLGQREASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAY 120 RALRQS QLRLSLFLGQREASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAY Sbjct: 61 RALRQSAQLRLSLFLGQREASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAY 120 Query: 121 PIDDVDLELDHAWQLSLRDAVALAARIERAALAVSSAVPQIERAVLFSSRTRRILANSEG 180 P DDVDLELDHAWQL LRDAVAL ARIERAALAVSSAVPQIE+AVLFSSRTRR+LANSEG Sbjct: 121 PSDDVDLELDHAWQLGLRDAVALVARIERAALAVSSAVPQIEQAVLFSSRTRRVLANSEG 180 Query: 181 FLRSSASTRHSLSCTAVAIRDGAKEIESWWDARRDPAQLPSPESIGACAGRRALARLGAR 240 FLRSSASTRHSLSCTAVAIRDGAKEIESWWDARRDPAQLPSPES+GACAGRRALARLGAR Sbjct: 181 FLRSSASTRHSLSCTAVAIRDGAKEIESWWDARRDPAQLPSPESLGACAGRRALARLGAR 240 Query: 241 QVATQTCPVLFEPQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLL 300 QVATQTCPVLFEPQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLL Sbjct: 241 QVATQTCPVLFEPQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLL 300 Query: 301 KGGMGSRSYDGNGAALRPRTLIAGGRLQGYYLDAYGARRIGLPLVEHGAGPSNVMVSSTT 360 KGGMGSRSYDGNGAALRPRTLIAGGRLQGYYL AYGARR+GLPL EHGAGPSNVMVSSTT Sbjct: 301 KGGMGSRSYDGNGAALRPRTLIAGGRLQGYYLGAYGARRLGLPLAEHGAGPSNVMVSSTT 360 Query: 361 SEASGDLEAMIRRIGTGLVVSGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVAS 420 S ASGDLEAMIRR+GTGLV+SGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVAS Sbjct: 361 SAASGDLEAMIRRMGTGLVISGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVAS 420 Query: 421 NLREMFAGIVAVGNDRLTQNGTTTGSWLIDKMRVGGI 457 NLREMFAGIVAVGNDRLTQNGTTTGSWLIDKMRVGGI Sbjct: 421 NLREMFAGIVAVGNDRLTQNGTTTGSWLIDKMRVGGI 457 >gi|300311183|ref|YP_003775275.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 454 Score = 326 bits (836), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 176/444 (39%), Positives = 258/444 (58%), Gaps = 4/444 (0%) Query: 17 QQYFETIAQQTLEVARRHGATAAAVQIEEVESLSASVQGGRPNERALRQSTQLRLSLFLG 76 Q + +AQ L AR GA+ AAV I E LS +V+ G+ + + +++FLG Sbjct: 10 QDQLKQLAQDVLRYARDKGASDAAVDISEGSGLSVAVRKGKIETIEQNKDKGIGVTVFLG 69 Query: 77 Q----REASVSSSDLSQQGVAALVARGMDSAAIAAEDQHAGLPGLSAYPIDDVDLELDHA 132 + R + S+SD SQQ + V + A + D AGLP + DL+L Sbjct: 70 EGRQVRRGNASTSDFSQQALKETVEAAYNIARFTSIDDCAGLPDAEMLEMAPRDLKLFSP 129 Query: 133 WQLSLRDAVALAARIERAALAVSSAVPQIERAVLFSSRTRRILANSEGFLRSSASTRHSL 192 W +S +AVALA R E AAL + E A ++ + I ANS GF+ +RH++ Sbjct: 130 WNISAEEAVALAQRCETAALETDPRISNSEGAGVYVQHSHFIAANSRGFMGGYPFSRHTI 189 Query: 193 SCTAVAIRDGAKEIESWWDARRDPAQLPSPESIGACAGRRALARLGARQVATQTCPVLFE 252 S +A + + + + W+ ++RDP +L PE+IG A RALARL AR+++T+ CPVLFE Sbjct: 190 SVAPIAGKGASMQRDDWYSSKRDPKKLAQPEAIGRYAAERALARLNARKLSTRKCPVLFE 249 Query: 253 PQAAASLLNEFVQAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGN 312 AA LL FVQA+S LY S+FL D+LGQ+VF H+ I +DP + GG+GS +D Sbjct: 250 APLAAGLLGAFVQAVSGGALYRKSTFLLDSLGQQVFPEHIQILEDPHVIGGVGSAPFDEE 309 Query: 313 GAALRPRTLIAGGRLQGYYLDAYGARRIGLPLVEHGAGPSNVMVSSTTSEASGDLEAMIR 372 G R ++ G +QGY+L Y AR++G+ + G N+ + S + + + M++ Sbjct: 310 GVRTLKREVVRNGVVQGYFLSTYSARKLGMQTTGNSGGSHNLTLRSELTTRADTFKGMLK 369 Query: 373 RIGTGLVVSGLNGQGVNLMTGDFSRACSGFWVENGQIAYPVAGITVASNLREMFAGIVAV 432 ++GTGL+V+ L GQGVN +TGD+SR SG+WVENG I YPV IT+A N+++M IVAV Sbjct: 370 KMGTGLLVTELMGQGVNYVTGDYSRGASGYWVENGVIQYPVEEITIAGNMKDMLRQIVAV 429 Query: 433 GNDRLTQNGTTTGSWLIDKMRVGG 456 GND L + TGS L++ M + G Sbjct: 430 GNDTLIRGTKETGSILLESMTIAG 453 >gi|300312123|ref|YP_003776215.1| Zn-dependent protease TldD protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 117/424 (27%), Positives = 182/424 (42%), Gaps = 29/424 (6%) Query: 31 ARRHGATAAAVQIEEVESLSASVQGGRPNERALRQSTQLRLSLFLGQREASVSSS--DLS 88 A R G A + ++ + S ++ G+ QS L L G++ S+ D + Sbjct: 51 ALRKGGDYADLYLKSSVAESWHLERGKVTRSNYNQSQGFGLRLVEGEQSVLTSADRFDAT 110 Query: 89 QQGVAA-LVARGMDSAAIAAEDQHAGLP--GLSAYPIDDVDLELDHAWQLSLRDAVALAA 145 G AA L A + A ++ LP G + Y +DD LD A +++L ++V Sbjct: 111 TIGKAADLAADAVGHAPVSPAGLVLKLPKRGPTYYTLDDPLPSLDAAHKIALLESV---- 166 Query: 146 RIERAALAVSSAVPQIERAVLFSSRTRRILANSEGFLRSSASTRHSLSCTAVAIRDGAKE 205 +RA A S V ++ A L S LA ++G+ L +R+G +E Sbjct: 167 --DRACRARSPLVVEV-MASLSISLDLVFLARNDGYCGGDVRPLVRLDVAVQVVRNGRRE 223 Query: 206 IESWWDARR------DPAQLPSPESIGACAGRRALARLGARQVATQTCPVLFEPQAAASL 259 S R D A + ES+ A A AL +L AR T V+ P + Sbjct: 224 GASRTLGGRVGLATFDHAAI---ESVAALAVDAALIKLEARAAPAGTMTVVIGPGWNGVM 280 Query: 260 LNEFV-QAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAALRP 318 L+E V + SS +GQ V A + I DD + G GS + D G + Sbjct: 281 LHEAVGHGLEGDFNRRGSSAFAGRIGQRVAAPGVTIVDDGTVPGSRGSLNIDDEGTPGQC 340 Query: 319 RTLIAGGRLQGYYLDAYGARRIGLPLVEHGAGPS-NVM----VSSTTSEASG-DLEAMIR 372 TLI G L Y D+ AR +G+ +G S +V+ +++T A G D +I Sbjct: 341 TTLIEDGILVSYMQDSLNARLMGVANTGNGRRESYDVLPMPRMTNTYMRAGGVDPAEIIA 400 Query: 373 RIGTGLVVSGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGIVA 431 + G+ ++ L G V++ +G F A S F +ENG++ PV G TV N + + Sbjct: 401 SVKQGIYIAHLEGGQVDISSGRFMFAASEAFLIENGRLGAPVKGATVLGNGPQSLKNVKL 460 Query: 432 VGND 435 +GND Sbjct: 461 IGND 464 >gi|300310755|ref|YP_003774847.1| Zn-dependent protease [Herbaspirillum seropedicae SmR1] Length = 486 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 77/313 (24%), Positives = 125/313 (39%), Gaps = 12/313 (3%) Query: 141 VALAARIERAALAVSSAVPQIERAVLFSSRTRRILANSEGFLRSSASTRHSLSCTAVAIR 200 V L R+ER A A V Q+ A L ++A ++G L + +S T +A + Sbjct: 145 VQLLERVERIARAKDPRVKQV-MASLSGEYDVVLVARADGVLAADIRPLVRVSVTVIAEQ 203 Query: 201 DGAKEIESWWDARRDPAQLPSP---ESIGACAGRRALARLGARQVATQTCPVLFEPQAAA 257 +G +E+ S R S E + A AL L +R V+ P Sbjct: 204 NGRREMGSSGGGGRYDYGYFSDVLLEQYASEAVATALVNLESRPAPAGPMTVVLGPGWPG 263 Query: 258 SLLNEFV-QAISARPLYMHSSFLRDALGQEVFAAHMNISDDPLLKGGMGSRSYDGNGAAL 316 LL+E + + SS +G+ V A + + DD + GS + D G Sbjct: 264 ILLHEAIGHGLEGDFNRKGSSTFSGRIGERVAAKGVTVVDDGTIADRRGSLNIDDEGNPT 323 Query: 317 RPRTLIAGGRLQGYYLDAYGARRIGLPLV------EHGAGPSNVMVSSTTSEASGDLEAM 370 + TLI G L+GY D AR + + + P M ++ D + Sbjct: 324 QCTTLIEDGILKGYIQDTMNARLMKMDVTGNARRESFAHLPMPRMTNTYMLGGDRDPAEI 383 Query: 371 IRRIGTGLVVSGLNGQGVNLMTGDFSRACS-GFWVENGQIAYPVAGITVASNLREMFAGI 429 + + GL G V++ G F + S + +E+G+I YPV G T+ N ++ + Sbjct: 384 LASVKNGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLIGNGPDVLNRV 443 Query: 430 VAVGNDRLTQNGT 442 +GND +G Sbjct: 444 SMIGNDMRLDSGV 456 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,345,671 Number of extensions: 55815 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 4 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)